Dihydrotanshinone I inhibits angiogenesis both in vitro and in vivo
Weipeng Bian
School of Life Science, East China Normal University, Shanghai 200062, China
National Laboratory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai Jiaotong University, Shanghai 200240, China
Search for more papers by this authorCorresponding Author
Fei Chen
National Laboratory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai Jiaotong University, Shanghai 200240, China
*Corresponding author: Tel /Fax, 86-21-34206022; E-mail, [email protected]Search for more papers by this authorLing Bai
Institute of Mechanobiology and Medical Engineering, Shanghai Jiaotong University, Shanghai 200240, China
Search for more papers by this authorPing Zhang
National Laboratory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai Jiaotong University, Shanghai 200240, China
Search for more papers by this authorWenxin Qin
National Laboratory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai Jiaotong University, Shanghai 200240, China
Search for more papers by this authorWeipeng Bian
School of Life Science, East China Normal University, Shanghai 200062, China
National Laboratory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai Jiaotong University, Shanghai 200240, China
Search for more papers by this authorCorresponding Author
Fei Chen
National Laboratory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai Jiaotong University, Shanghai 200240, China
*Corresponding author: Tel /Fax, 86-21-34206022; E-mail, [email protected]Search for more papers by this authorLing Bai
Institute of Mechanobiology and Medical Engineering, Shanghai Jiaotong University, Shanghai 200240, China
Search for more papers by this authorPing Zhang
National Laboratory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai Jiaotong University, Shanghai 200240, China
Search for more papers by this authorWenxin Qin
National Laboratory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai Jiaotong University, Shanghai 200240, China
Search for more papers by this authorThis work was supported by the grants from the Foundation of Shanghai Municipal Health Bureau (No. 287), and the National High Technology Research and Development Program of China (No. 2006AA020501)
Abstract
Dihydrotanshinone I (DI), a naturally occurring compound extracted from Salvia miltiorrhiza Bunge, has been reported to have cytotoxicity to a variety of tumor cells. In this study, we investigated its anti-angiogenic capacity in human umbilical vein endothelial cells. DI induced a potent cytotoxicity to human umbilical vein endothelial cells, with an IC50 value of approximately 1.28 μg/ml. At 0.25-1 μg/ml, DI dose-dependently suppressed human umbilical vein endothelial cell migration, invasion, and tube formation detected by wound healing, Transwell invasion and Matrigel tube formation assays, respectively. Moreover, DI showed significant in vivo anti-angiogenic activity in chick embryo chorioallantoic membrane assay. DI induced a 61.1% inhibitory rate of microvessel density at 0.2 μg/egg. Taken together, our results showed that DI could inhibit angio-genesis through suppressing endothelial cell proliferation, migration, invasion and tube formation, indicating that DI has a potential to be developed as a novel anti-angiogenic agent.
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