Enhanced Invasive and Metastatic Potential Induced by Transforming Growth Factor-β1 Might be Correlated with Glutathione-S-transferase-π, Cofilin and Heat Shock Protein 27 in SGC-7901 Gastric Cancer Cells
Kuansong WANG
Department of Pathology, Basic School of Medicine, Central South University, Changsha 410013, China
These authors contributed equally to this work
Search for more papers by this authorJinghe LI
Department of Pathology, Basic School of Medicine, Central South University, Changsha 410013, China
These authors contributed equally to this work
Search for more papers by this authorChangli ZHEN
Department of Pathology, Basic School of Medicine, Central South University, Changsha 410013, China
Search for more papers by this authorJianhua ZHOU
Department of Pathology, Basic School of Medicine, Central South University, Changsha 410013, China
Search for more papers by this authorDesheng XIAO
Department of Pathology, Basic School of Medicine, Central South University, Changsha 410013, China
Search for more papers by this authorJie LIU
Department of Pathology, Basic School of Medicine, Central South University, Changsha 410013, China
Search for more papers by this authorYing LIU
Department of Pathology, Basic School of Medicine, Central South University, Changsha 410013, China
Search for more papers by this authorHaiying JIANG
Department of Pathology, Basic School of Medicine, Central South University, Changsha 410013, China
Search for more papers by this authorChen CHEN
Department of Pathology, Basic School of Medicine, Central South University, Changsha 410013, China
Search for more papers by this authorCorresponding Author
Jifang WEN
Department of Pathology, Basic School of Medicine, Central South University, Changsha 410013, China
*Corresponding author: Tel, 86-731-2355098; Fax, 86-731-2650400; E-mail, [email protected]Search for more papers by this authorKuansong WANG
Department of Pathology, Basic School of Medicine, Central South University, Changsha 410013, China
These authors contributed equally to this work
Search for more papers by this authorJinghe LI
Department of Pathology, Basic School of Medicine, Central South University, Changsha 410013, China
These authors contributed equally to this work
Search for more papers by this authorChangli ZHEN
Department of Pathology, Basic School of Medicine, Central South University, Changsha 410013, China
Search for more papers by this authorJianhua ZHOU
Department of Pathology, Basic School of Medicine, Central South University, Changsha 410013, China
Search for more papers by this authorDesheng XIAO
Department of Pathology, Basic School of Medicine, Central South University, Changsha 410013, China
Search for more papers by this authorJie LIU
Department of Pathology, Basic School of Medicine, Central South University, Changsha 410013, China
Search for more papers by this authorYing LIU
Department of Pathology, Basic School of Medicine, Central South University, Changsha 410013, China
Search for more papers by this authorHaiying JIANG
Department of Pathology, Basic School of Medicine, Central South University, Changsha 410013, China
Search for more papers by this authorChen CHEN
Department of Pathology, Basic School of Medicine, Central South University, Changsha 410013, China
Search for more papers by this authorCorresponding Author
Jifang WEN
Department of Pathology, Basic School of Medicine, Central South University, Changsha 410013, China
*Corresponding author: Tel, 86-731-2355098; Fax, 86-731-2650400; E-mail, [email protected]Search for more papers by this authorThis work was supported partly by a grant from the Innovation Project of Central South University (No. 2340-75237)
Abstract
In our previous study, we found that the transforming growth factor (TGF)-β1 enhanced the metastatic and invasive potential of gastric cancer cells. Proteomics was employed in this study to further illustrate the underlying molecular mechanisms. After two-dimensional electrophoresis, image analysis, spot identification, protein identification and database analysis, three proteins, namely, glutathione-S-transferase-π (GST-π), cofilin and heat shock protein 27 (HSP27), were found to be up-regulated in TGF-β1 treated SGC-7901 cells. The findings were further confirmed by Western blot analysis. These results suggested that GST-π, cofilin and HSP27 might participate in enhanced invasive potential induced by TGF-β1.
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