Identification of a Positive Cis-Element Upstream of Human NKX3.1 Gene
This work was supported by the grants from the National Natural Science Foundation of China (No. 30470952) and the Natural Science Foundation of Shandong Province (No. Y2004C26)
Abstract
Abstract NKX3.1 is a prostate-specific homeobox gene related to prostate development and prostate cancer. In this work, we aimed to identify precisely the functional cis-element in the 197 bp region (from −1032 to −836 bp) of the NKX3.1 promoter (from −1032 to +8 bp), which was previously identified to present positive regulatory activity on NKX3.1 expression, by deletion mutagenesis analysis and electrophoretic mobility shift assay (EMSA). A 16 bp positive cis-element located between −920 and −905 bp upstream of the NKX3.1 gene was identified by deletion mutation analysis and proved to be a functional positive cis-element by EMSA. It will be important to further study the functions and regulatory mechanisms of this positive cis-element in NKX3.1 gene expression.
Edited by Ding-Gan LIU
