Volume 9, Issue 2 pp. 189-198

Expression and localization of CKLFSF2 in human spermatogenesis

Gang Liu

Gang Liu

Andrology Center of Peking University First Hospital, Beijing 100009, China

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Zhong-Cheng Xin

Zhong-Cheng Xin

Andrology Center of Peking University First Hospital, Beijing 100009, China

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Liang Chen

Liang Chen

Andrology Center of Peking University First Hospital, Beijing 100009, China

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Long Tian

Long Tian

Andrology Center of Peking University First Hospital, Beijing 100009, China

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Yi-Ming Yuan

Yi-Ming Yuan

Andrology Center of Peking University First Hospital, Beijing 100009, China

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Wei-Dong Song

Wei-Dong Song

Andrology Center of Peking University First Hospital, Beijing 100009, China

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Xue-Jun Jiang

Xue-Jun Jiang

Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China

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Ying-Lu Guo

Ying-Lu Guo

Andrology Center of Peking University First Hospital, Beijing 100009, China

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Aleksander Giwercman

Aleksander Giwercman

Andrology Center of Peking University First Hospital, Beijing 100009, China

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First published: 15 March 2007
Citations: 3
Dr Zhong-Cheng Xin, Andrology Center of Peking University First Hospital, Beijing 100009, China. Tel/Fax: +86-10-6618-2822 E-mail: [email protected]

Abstract

Aim: To investigate the expression and subcellular localization of chemokine-like factor superfamily 2 (CKLFSF2) in human testis and its potential role in spermatogenesis. Methods: A specific polyclonal antibody against CKLFSF2 was raised. The expression and cellular localization of CKLFSF2 in the seminiferous tubules was checked by immunohistochemistry method. Also, in situ hybridization was applied to localize the mRNA distribution. The EGFP-CKLFSF2 fusion protein was expressed in COS-7 cells to localize its subcellular location in vitro. In addition, the abnormal expression of CKLFSF2 in testes of patients with male infertility was assayed by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry methods. Results: Having a close correlation with spermatogenesis defects, CKLFSF2 was specifically expressed in meiotic and post-meiotic germ cells, which were localized to the endoplasmic reticulum (ER) near the Golgi apparatus. Conclusion: CKLFSF2 could play important roles in the process of meiosis and spermiogenesis, and might be involved in the vesicular transport or membrane apposition events in the endoplasmic reticulum.

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