Downregulation of STEAP4, a highly-expressed TNF-α-inducible gene in adipose tissue, is associated with obesity in humans¹

Chun-mei ZHANG

Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210004, China

Institute of Pediatrics, Nanjing Medical University, Nanjing 210029, China

These authors contributed equally to this work.

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Xia CHI,

Xia CHI

Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210004, China

Institute of Pediatrics, Nanjing Medical University, Nanjing 210029, China

These authors contributed equally to this work.

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Bin WANG,

Bin WANG

Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210004, China

Institute of Pediatrics, Nanjing Medical University, Nanjing 210029, China

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Min ZHANG,

Min ZHANG

Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210004, China

Institute of Pediatrics, Nanjing Medical University, Nanjing 210029, China

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Yu-hui NI,

Yu-hui NI

Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210004, China

Institute of Pediatrics, Nanjing Medical University, Nanjing 210029, China

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Rong-hua CHEN,

Rong-hua CHEN

Institute of Pediatrics, Nanjing Medical University, Nanjing 210029, China

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Xiao-nan LI,

Corresponding Author

Xiao-nan LI

Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210004, China

Institute of Pediatrics, Nanjing Medical University, Nanjing 210029, China

Correspondence to Dr Xi-rong GUO and Dr Xiao-nan LI. Phn 86-25-8686-2996. E-mail [email protected] (Xi-rong GUO) Phn 86-25-8686-2996. E-mail [email protected] (Xiao-nan LI)Search for more papers by this author

Xi-rong GUO,

Corresponding Author

Xi-rong GUO

Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210004, China

Institute of Pediatrics, Nanjing Medical University, Nanjing 210029, China

Chun-mei ZHANG,

Chun-mei ZHANG

Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210004, China

Institute of Pediatrics, Nanjing Medical University, Nanjing 210029, China

These authors contributed equally to this work.

Search for more papers by this author

Xia CHI,

Xia CHI

Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210004, China

Institute of Pediatrics, Nanjing Medical University, Nanjing 210029, China

These authors contributed equally to this work.

Search for more papers by this author

Bin WANG,

Bin WANG

Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210004, China

Institute of Pediatrics, Nanjing Medical University, Nanjing 210029, China

Search for more papers by this author

Min ZHANG,

Min ZHANG

Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210004, China

Institute of Pediatrics, Nanjing Medical University, Nanjing 210029, China

Search for more papers by this author

Yu-hui NI,

Yu-hui NI

Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210004, China

Institute of Pediatrics, Nanjing Medical University, Nanjing 210029, China

Search for more papers by this author

Rong-hua CHEN,

Rong-hua CHEN

Institute of Pediatrics, Nanjing Medical University, Nanjing 210029, China

Search for more papers by this author

Xiao-nan LI,

Corresponding Author

Xiao-nan LI

Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210004, China

Institute of Pediatrics, Nanjing Medical University, Nanjing 210029, China

Xi-rong GUO,

Corresponding Author

Xi-rong GUO

Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210004, China

Institute of Pediatrics, Nanjing Medical University, Nanjing 210029, China

First published: 27 April 2008

https://doi.org/10.1111/j.1745-7254.2008.00793.x

Citations: 4

Project supported by grants from the National Natural Science Foundation of China (No 30772364), the Natural Science Foundation of Jiangsu Province, China (No BK2007230), the Foundation of Ministry of Education, China (No 20070312001), and Nanjing Medical University (No 07NMUZ024).

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Abstract

Aim: To determine the relationship between six-transmembrane epithelial antigen of the prostate 4 (STEAP4) expression and obesity. Methods: RT–PCR and immunoblot analyses were performed to determine the differential expressions of STEAP4 mRNA and protein, respectively, in human omental adipose tissue from obese patients and normal weight controls. The expression pattern of STEAP4 mRNA in various human tissues was determined by RT–PCR. The subcellular localization of the STEAP4 protein in human adipose tissue was confirmed by immunohistochemistry. Finally, we confirmed that cultured human omental adipose tissue undergoes TNF-α-mediated regulation of the STEAP4 expression. Results: STEAP4 mRNA and protein levels were downregulated in omental adipose tissue from obese patients relative to normal controls. The STEAP4 expression was most abundant in human adipose tissue. An immunohistochemical analysis confirmed that STEAP4 was associated with the plasma membrane of adipocytes. The STEAP4 expression was induced by TNF-α in a dose-dependent manner in human adipose tissue. Conclusion: STEAP4 was abundantly expressed in human adipose tissue, and the STEAP4 expression was significantly downregulated in obese patients. STEAP4 localized to the plasma membrane of adipocytes, and the STEAP4 expression was induced by TNF-α in adipose tissue. These data suggest that STEAP4 may play a significant role in the development of human obesity.

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Volume29, Issue5

May 2008

Pages 587-592

Downregulation of STEAP4, a highly-expressed TNF-α-inducible gene in adipose tissue, is associated with obesity in humans¹

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Downregulation of STEAP4, a highly-expressed TNF-α-inducible gene in adipose tissue, is associated with obesity in humans1

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Downregulation of STEAP4, a highly-expressed TNF-α-inducible gene in adipose tissue, is associated with obesity in humans¹