DIRECT DETERMINATION OF TRANS-OCTADECENOIC ACID IN DIET COMPOSITES USING A COMBINATION OF SILVER ION-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND CAPILLARY GAS CHROMATOGRAPHY
Corresponding Author
K.M. PHILLIPS
Department of Biochemistry Virginia Polytechnic Institute and State University Blacksburg, VA 24061–0308
1Corresponding author. (TEL) 540–231–9960, (FAX) 540–231–9070, [email protected]Search for more papers by this authorR.D. LUGOGO
Department of Biochemistry Virginia Polytechnic Institute and State University Blacksburg, VA 24061–0308
Search for more papers by this authorR.F. HARRIS
Department of Biochemistry Virginia Polytechnic Institute and State University Blacksburg, VA 24061–0308
Search for more papers by this authorM.-T. TARRAGÓ-TRANI
Department of Biochemistry Virginia Polytechnic Institute and State University Blacksburg, VA 24061–0308
Search for more papers by this authorJ.A. BAILEY
Department of Biochemistry Virginia Polytechnic Institute and State University Blacksburg, VA 24061–0308
Search for more papers by this authorK.K. STEWART
Department of Biochemistry Virginia Polytechnic Institute and State University Blacksburg, VA 24061–0308
Search for more papers by this authorCorresponding Author
K.M. PHILLIPS
Department of Biochemistry Virginia Polytechnic Institute and State University Blacksburg, VA 24061–0308
1Corresponding author. (TEL) 540–231–9960, (FAX) 540–231–9070, [email protected]Search for more papers by this authorR.D. LUGOGO
Department of Biochemistry Virginia Polytechnic Institute and State University Blacksburg, VA 24061–0308
Search for more papers by this authorR.F. HARRIS
Department of Biochemistry Virginia Polytechnic Institute and State University Blacksburg, VA 24061–0308
Search for more papers by this authorM.-T. TARRAGÓ-TRANI
Department of Biochemistry Virginia Polytechnic Institute and State University Blacksburg, VA 24061–0308
Search for more papers by this authorJ.A. BAILEY
Department of Biochemistry Virginia Polytechnic Institute and State University Blacksburg, VA 24061–0308
Search for more papers by this authorK.K. STEWART
Department of Biochemistry Virginia Polytechnic Institute and State University Blacksburg, VA 24061–0308
Search for more papers by this authorABSTRACT
Silver ion-high performance liquid chromatography (Ag-HPLC) and capillary gas chromatography (GC) were combined to quantify total trans-octadecenoic acid (18:1t) in diet composites and oils. The 18:1 cis and trans fatty acid methyl esters (FAME) of saponified and methylated (BF3/methanol) oils and food composite total lipid extracts were separated by Ag-HPLC (ChromSpher5 Lipids column); total 18:1t in the trans fraction was quantified by GC (105m × 0.25mm SP-2560 capillary column). Positional isomers 18:1c and 18:1t which coeluted in direct GC were clearly separated by preparatory Ag-HPLC. Quantitative recovery of 18:1t from a standard mixture of FAME, an oil, and a diet composite was obtained for additions of 4–57 mg. The analytical range for total 18:1t was 1.6–260 mg/mL per aliquot of total lipid extract or oil. Relative standard deviations were < 6% for food composites and < 3% for oils and pure standards.
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