Plasma Separation by Centrifugation and Subsequent Plasma Filtration: Impact on Survival in a Pig Model of Sepsis

Corresponding Author

Martin Sauer

Department of Anesthesiology and Intensive Care Medicine

Mr Martin Sauer, Department of Anesthesiology and Intensive Care Medicine, University of Rostock, Schillingallee 35, 18057 Rostock, Germany. Email: [email protected]Search for more papers by this author

Jens Altrichter,

Jens Altrichter

Department of Medicine, Division of Nephrology

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Thomas Mencke,

Thomas Mencke

Department of Anesthesiology and Intensive Care Medicine

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Sven Klöhr,

Sven Klöhr

Department of Anesthesiology, Hospital Bamberg, Bamberg, Germany

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Maren Thomsen,

Maren Thomsen

Department of Anesthesiology and Intensive Care Medicine

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Hans J. Kreutzer,

Hans J. Kreutzer

Department of Pathology, Medical Faculty of the University of Rostock, Rostock

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Gabriele Nöldge-Schomburg,

Gabriele Nöldge-Schomburg

Department of Anesthesiology and Intensive Care Medicine

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Steffen R. Mitzner,

Steffen R. Mitzner

Department of Medicine, Division of Nephrology

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Martin Sauer,

Corresponding Author

Martin Sauer

Department of Anesthesiology and Intensive Care Medicine

Jens Altrichter,

Jens Altrichter

Department of Medicine, Division of Nephrology

Search for more papers by this author

Thomas Mencke,

Thomas Mencke

Department of Anesthesiology and Intensive Care Medicine

Search for more papers by this author

Sven Klöhr,

Sven Klöhr

Department of Anesthesiology, Hospital Bamberg, Bamberg, Germany

Search for more papers by this author

Maren Thomsen,

Maren Thomsen

Department of Anesthesiology and Intensive Care Medicine

Search for more papers by this author

Hans J. Kreutzer,

Hans J. Kreutzer

Department of Pathology, Medical Faculty of the University of Rostock, Rostock

Search for more papers by this author

Gabriele Nöldge-Schomburg,

Gabriele Nöldge-Schomburg

Department of Anesthesiology and Intensive Care Medicine

Search for more papers by this author

Steffen R. Mitzner,

Steffen R. Mitzner

Department of Medicine, Division of Nephrology

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First published: 05 March 2012

https://doi.org/10.1111/j.1744-9987.2011.01055.x

Citations: 5

Financial support: This study relied solely on financial resources of the University of Rostock.

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Abstract

The impact on survival of a combination of plasma separation by centrifugation and subsequent plasma filtration was tested in a bacterial sepsis model in pigs. In this animal study 19 pigs were included. Groups II and III received an intravenous lethal dose of live Staphylococcus aureus over 1 h; group I received saline (non-septic control—NC). Groups I and II were treated by an extracorporeal circuit consisting of online centrifugation and subsequent plasma filtration (group II: treated group—TG) for 4 h; group III had no specific treatment (septic control, SC). The observation time was 7 days. All animals of group I (NC) and group II (TG) survived, while all animals of group III (SC) died during the observation time. Extracorporeal therapy with online centrifugation and plasma filtration significantly improved survival in a pig model of sepsis. Further studies with this approach are encouraged.

REFERENCES

1 Angus DC, Linde-Zwirble WT, Lidicker J et al. Epidemiology of severe sepsis in the United States: analysis of incidence, outcome, and associated costs of care. Crit Care Med 2001; 29: 1303–10.
10.1097/00003246-200107000-00002
CAS PubMed Web of Science® Google Scholar
2 Dellinger RP, Levy MM, Carlet JM et al. Surviving Sepsis Campaign. International guidelines for management of severe sepsis and septic shock: 2008. Intensive Care Med 2008; 36: 296–327.
PubMed Web of Science® Google Scholar
3 Rivers E, Nguyen B, Havstad S et al. Early Goal-Directed Therapy Collaborative Group. Early goal-directed therapy in the treatment of severe sepsis and septic shock. N Engl J Med 2001; 345: 1368–77.
10.1056/NEJMoa010307
CAS PubMed Web of Science® Google Scholar
4 Ronco C, Tetta C, Mariano F et al. Interpreting the mechanisms of continuous renal replacement therapy in sepsis: the peak concentration hypothesis. Artif Organs 2003; 27: 792–801.
10.1046/j.1525-1594.2003.07289.x
CAS PubMed Web of Science® Google Scholar
5 Rimmelé T, Kellum JA. Clinical review: blood purification for sepsis. Crit Care 2011; 15: 205.
10.1186/cc9411
PubMed Web of Science® Google Scholar
6 Busund R, Koukline V, Utrobin U et al. Plasmapheresis in severe sepsis and septic shock: a prospective, randomised, controlled trial. Intensive Care Med 2002; 28: 1434–39.
10.1007/s00134-002-1410-7
PubMed Web of Science® Google Scholar
7 Iwai H, Nakaki M, Naito T et al. Removal of endotoxin and cytokines by plasma exchange in patients with acute hepatic failure. Crit Care Med 1998; 26: 873–6.
10.1097/00003246-199805000-00021
CAS PubMed Web of Science® Google Scholar
8 Van Deuren M, Frieling JT, van der Ven-Jongekrijg J et al. Plasma patterns of tumor necrosis factor-alpha (TNF) and TNF soluble receptors during acute meningococcal infections and the effects of plasma exchange. Clin Infect Dis 1998; 26: 918–23.
10.1086/513933
CAS PubMed Web of Science® Google Scholar
9 Lee PA, Weger GW, Pryor RW et al. Effects of filter pore-size on efficacy of continuous arteriovenous hemofiltration therapy for Staphylococcus aureus-induced septicaemia in immature swine. Crit Care Med 1998; 26: 730–7.
10.1097/00003246-199804000-00024
CAS PubMed Web of Science® Google Scholar
10 Lee PA, Matson JR, Pryor RW, Hinshaw LB. Continuous arteriovenous hemofiltration therapy for Staphylococcus aureus-induced septicemia in immature swine. Crit Care Med 1993; 21: 914–24.
10.1097/00003246-199306000-00022
PubMed Web of Science® Google Scholar
11 Hinshaw LB, Taylor FB, Chang ACK et al. Staphylococcus aureus-induced Shock: a pathophysiologic study. Circ Shock 1988; 26: 257–65.
CAS PubMed Web of Science® Google Scholar
12 Ronco C, Inguaggiato P, D'Intini V et al. The role of extracorporeal therapies in sepsis. J Nephrol 2003; 16: 34–41.
CAS PubMed Web of Science® Google Scholar
13 Bellomo R, Honoré PM, Matson J et al. Extracorporeal blood treatment (EBT) methods in SIRS/Sepsis. Int J Artif Organs 2005; 28: 450–8.
10.1177/039139880502800505
CAS PubMed Web of Science® Google Scholar
14 Cole L, Bellomo R, Hart G et al. A phase II randomized, controlled trial of continuous hemofiltration in sepsis. Crit Care Med 2002; 30: 100–6.
10.1097/00003246-200201000-00016
CAS PubMed Web of Science® Google Scholar
15 Payen D, Mateo J, Cavaillon JM et al. Impact of continuous venovenous hemofiltration on organ faiure during the early phase of severe sepsis: a randomized controlled trial. Crit Care Med 2009; 37: 803–10.
10.1097/CCM.0b013e3181962316
PubMed Web of Science® Google Scholar
16 Sykora R, Chvojka J, Krouzecky A et al. Coupled plasma filtration adsorption in experimental peritonitis-induced septic shock. Shock 2009; 31: 473–80.
10.1097/SHK.0b013e318188dec5
CAS PubMed Web of Science® Google Scholar
17 Eguchi Y. Plasma dia–filtration for severe sepsis. Contrib Nephrol 2010; 166: 142–9.
10.1159/000314864
PubMed Web of Science® Google Scholar
18 Reeves JH, Butt WW, Shann F et al. Continuous plasmafiltration in sepsis syndrome. Plasmafiltration in Sepsis Study Group. Crit Care Med 1999; 27: 2096–104.
10.1097/00003246-199910000-00003
CAS PubMed Web of Science® Google Scholar
19 Levy H, Ash SR, Knab W et al. Systemic inflammatory response syndrome treatment by powdered sorbent pheresis: the BioLogic-Detoxification Plasma Filtration System. ASAIO J 1998; 44: M659–65.
10.1097/00002480-199809000-00072
CAS PubMed Web of Science® Google Scholar
20 Ronco C, Brendolan A, Lonneman G et al. A pilot study of coupled plasma filtration with adsorption in septic shock. Crit Care Med 2002; 30: 1250–5.
10.1097/00003246-200206000-00015
PubMed Web of Science® Google Scholar

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Volume16, Issue3

June 2012

Pages 205-212

Plasma Separation by Centrifugation and Subsequent Plasma Filtration: Impact on Survival in a Pig Model of Sepsis

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Plasma Separation by Centrifugation and Subsequent Plasma Filtration: Impact on Survival in a Pig Model of Sepsis

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