Decrease of Photosystem II Photochemistry in Arabidopsis ppt1 Mutant Is Dependent on Leaf Age
Jin-Fang Ma
Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, Lanzhou 730000, China
Search for more papers by this authorJin-Kui Guo
Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Photosynthesis Research Center, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China
Search for more papers by this authorLian-Wei Peng
Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, Lanzhou 730000, China
Search for more papers by this authorCui-Yun Chen
Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, Lanzhou 730000, China
Search for more papers by this authorCorresponding Author
Li-Xin Zhang
Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, Lanzhou 730000, China
Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Photosynthesis Research Center, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China
*Author for correspondence. Tel: +86 (0)10 6283 6256; Fax: +86 (0)10 8259 9384; E-mail: <[email protected]>.Search for more papers by this authorJin-Fang Ma
Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, Lanzhou 730000, China
Search for more papers by this authorJin-Kui Guo
Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Photosynthesis Research Center, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China
Search for more papers by this authorLian-Wei Peng
Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, Lanzhou 730000, China
Search for more papers by this authorCui-Yun Chen
Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, Lanzhou 730000, China
Search for more papers by this authorCorresponding Author
Li-Xin Zhang
Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, Lanzhou 730000, China
Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Photosynthesis Research Center, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China
*Author for correspondence. Tel: +86 (0)10 6283 6256; Fax: +86 (0)10 8259 9384; E-mail: <[email protected]>.Search for more papers by this authorSupported by Trans-Century Training Programme Foundation for the Talents, The Project-sponsored by SRF for ROCS and the Key Project of Chinese Ministry of Education.
Abstract
In the present study, we compared the effect of leaf age at one development stage on the photosynthetic behavior of a T-DNA-tagged allele of the ppt1 mutant. Chlorophyll fluorescence (Fv/Fm, where Fm is the maximum fluorescence yield and Fv is variable fluorescence) and fluorescence decay kinetics of the ppt1 mutant were different in leaves of different ages. The steady state levels of thylakoid membrane proteins in 40-day-old leaves were decreased compared with those in 20-day-old leaves and changes in photosystem (PS) II proteins were correlated with those of the Fv/Fm ratio in the ppt1 mutant. Increased accumulation of leaf sugars was accompanied by decreased photosynthetic gene transcripts and protein content in 40-day-old leaves of the mutant. Thus, the results of the present study provide evidence for the phosphate translocator in maintaining normal photosynthesis at a late leaf age.
(Managing editor: Ping He)
References
- Crafts-Brandner SJ, Below FE, Wittenbach VA, Harper JE, Hageman RH (1984). Differential senescence of maize hybrids following ear removal. II. Selected leaf. Plant Physiol 74, 368–373.
- Crofts AR, Wraight CA (1983). The electrochemical domain of photosynthesis. Biochim Biophys Acta 726, 149–185.
- Diner BA (1998). Photosynthesis: Molecular biology of energy capture. Methods Enzymol 297, 337–360.
- Fischer K, Kammerer B, Gutensohn M, Arbinger B, Weber A, Häusler RE et al. (1997). A new class of plastidic phosphate translocators, a putative link between primary and secondary metabolism by the phosphoenolpyruvate/phosphate antiporter. Plant Cell 9, 453–462.
- Flügge UI (1995). Phosphate translocation in the regulation of photosynthesis. J Exp Bot 46, 1317–1323.
- Gray GR, Hope BJ, Qin X, Taylor BG, Whitehead CL (2003). The characterization of photoinhibition and recovery during cold acclimation in Arabidopsis thaliana using chlorophyll fluorescence imaging. Physiol Plant 119, 365–375.
- Herrmann KM (1995). The shikimate pathway: Early steps in the biosynthesis of aromatic compounds. Plant Cell 7, 907–919.
- Knappe S, Flügge UI, Fischer K (2003a). Analysis of the plastidic phosphate translocator gene family in Arabidopsis and identification of new phosphate translocator-homologous transporters, classified by their putative substrate binding site. Plant Physiol 131, 1178–1190.
- Knappe S, Löttgert T, Schneider A, Voll L, Flügge UI, Fischer K (2003b). Characterization of two functional phosphoenolpyruvate/phosphate translocator (PPT) genes in Arabidopsis-AtPPT1 may be involved in the provision of signals for correct mesophyll development. Plant J 36, 411–420.
- Krapp A, Stitt M (1994). Influence of high carbohydrate content on the activity of plastidic and cytosolic isoenzyme pairs in photo-synthetic tissues. Plant Cell Environ 17, 861–866.
- Lara MV, Casati P, Andreo CS (2002). CO2-concentrating mechanisms in Egeria densa, a submersed aquatic plant. Physiol Plant 115, 487–495.
- Lazár D (1999). Chlorophyll a fluorescence induction. Biochim Biophys Acta 1412, 1–28.
- Li H, Culligan K, Dixon RA, Chory J (1995). CUE1, a mesophyll cell-specific positive regulator of light-controlled gene expression in Arabidopsis. Plant Cell 7, 1599–1610.
-
Lichtenthaler HK,
Wellburn AR (1983). Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents.
Biochem Soc Trans
603, 591–592.
10.1042/bst0110591 Google Scholar
- López-Juez E, Jarvis RP, Takeuchi A, Page AM, Chory J (1998). New Arabidopsis CAB-underexpressed (cue) mutants suggest a close connection between plastid- and phytochrome-regulation of nuclear gene expression. Plant Physiol 118, 803–815.
- Matthew J, Paul XX, Till K (2003). Pellny carbon metabolite feedback regulation of leaf photosynthesis and development. J Exp Bot 54, 539–547.
- Meetam M, Keren N, Ohad I, Pakrasi HB (1999). The PsbY is not essential for oxygenic photosynthesis in the cyanobacterium Synechocystis sp. PCC 6803. Plant Physiol 121, 1267–1272.
- Peng LW, Ma JF, Chi W, Guo JK, Zhu SY, Lu QT et al. (2006). Low PSII accumulation is involved in efficient assembly of photo-system II in Arabidopsis thaliana. Plant Cell 18, 955–969.
- Scholes JD, Lee PJ, Horton P, Lewis DH (1994). Invertase: Understanding changes in the photosynthetic and carbohydrate metabolism of barley leaves infected with powdery mildew. New Phytol 126, 213–222.
- Sheen J (1990). Metabolic repression of transcription in higher plants. Plant Cell 2, 1027–1038.
- Skotnica J, Matoušková M, Nauš J, Lazár D, Dvorák L (2000). Thermoluminescence and fluorescence study of changes in photosystem II photochemistry in desiccating barley leaves. Photosyn Res 65, 29–40.
- Strasser RJ, Srivastava A, Tsimili-Michael M (2000). The fluorescence transient as a tool to characterize and screen photosynthetic samples In: M Yunus, U Pathre, P Mohanty, eds. Probing Photosynthesis: Mechanism, Regulation and Adaption. Taylor Francis, London pp. 445–483.
- Streatfield SJ, Weber A, Kinsman EA, Hausler RE, Li J, Post-Beittenmiller D et al. (1999). The phosphoenolpyruvate/phosphate translocator is required for phenolic metabolism, palisade cell development and plastid-dependent nuclear gene expression. Plant Cell 11, 1609–1621.
- Trìlek M, Kramer DM, Koblizek M, Nedbal L (1997). Dual modulation LED kinetic fluometer. J Lumin 72-74, 597–599.
- Voll L, Häusler RE, Hecker R, Weber A, Weissenböck G, Waffenschmidt S et al. (2003). The phenotype of the Arabidopsis cue1 mutant is not simply caused by a general restriction of the shikimate pathway. Plant J 36, 301–317.
