Effects of Exogenous Spermidine on Photosystem II of Wheat Seedlings Under Water Stress
Hui-Guo Duan
Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University , Chengdu 610064, China
Department of Chemistry and Life Sciences, Neijiang Teachers' College , Neijiang 641112, China
Search for more papers by this authorShu Yuan
Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University , Chengdu 610064, China
Search for more papers by this authorWen-Juan Liu
Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University , Chengdu 610064, China
Search for more papers by this authorDe-Hui Xi
Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University , Chengdu 610064, China
Search for more papers by this authorDong-Hong Qing
Department of Chemistry and Life Sciences, Neijiang Teachers' College , Neijiang 641112, China
Search for more papers by this authorHou-Guo Liang
Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University , Chengdu 610064, China
Search for more papers by this authorCorresponding Author
Hong-Hui Lin
Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University , Chengdu 610064, China
*Author for correspondence. Tel: +86 (0)28 8541 1175; E-mail: <[email protected]>.Search for more papers by this authorHui-Guo Duan
Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University , Chengdu 610064, China
Department of Chemistry and Life Sciences, Neijiang Teachers' College , Neijiang 641112, China
Search for more papers by this authorShu Yuan
Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University , Chengdu 610064, China
Search for more papers by this authorWen-Juan Liu
Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University , Chengdu 610064, China
Search for more papers by this authorDe-Hui Xi
Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University , Chengdu 610064, China
Search for more papers by this authorDong-Hong Qing
Department of Chemistry and Life Sciences, Neijiang Teachers' College , Neijiang 641112, China
Search for more papers by this authorHou-Guo Liang
Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University , Chengdu 610064, China
Search for more papers by this authorCorresponding Author
Hong-Hui Lin
Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University , Chengdu 610064, China
*Author for correspondence. Tel: +86 (0)28 8541 1175; E-mail: <[email protected]>.Search for more papers by this authorSupported by the National Natural Science Foundation of China (30571119), the Sichuan Science and Technology Foundation (04ZQ026-036 and 05JY029-017), the Education Ministry Doctoral Foundation of China (20040610015), and the Chengdu Science and Technology Foundation (05HJSW117).
Abstract
The effects of exogenous spermidine (Spd) on lipid peroxidation, relative plasma membrane permeability, photosystem II (PSII) gene expression and PSII photochemical activity in water-stressed wheat seedlings were investigated. The decrease in relative water content (RWC), Chl content, and 2,6-dichlorophenol indophenol (DCIP) photoreduction of PSII, and increases in electrolyte leakage of plasma membranes and malonyldialdehyde (MDA) in water-stressed leaves was alleviated by Spd pretreatment. Furthermore, Western and Northern blot analysis showed that decreases in the PSII major proteins D1, D2 and LHCII and the transcripts of corresponding genes psbA, psbD and cab were also alleviated by Spd pretreatment under water stress. These results suggest that the application of exogenous Spd protects PSII against water stress at both the transcriptional level and the translational level, and allows PSII to retain a higher activity level during water stress. The protective role of Spd in the photosynthetic apparatus also is discussed.
(Managing editor: Ping He)
References
- Baker NR (1991). A possible role for photosystem II in environmental perturbation of photosynthesis. Physiol Plant 81, 563–570.
- Beigbeder A, Vavadakis M, Navakoudis E, Kotzabasis K (1995). Influence of polyamine inhibitors on light-independent and light-dependent chlorophyll biosynthesis and on the photosynthetic rate. J Photochem Photobiol B: Biol 28, 235–242.
- Besford RT, Richardson CM, Campos JL, Tiburcio AF (1993). Effect of polyamines on stabilization of molecular complexes in thylakoid membranes of osmotically stressed oat leaves. Planta 189, 201–206.
- Della Mea, M, Di Sandro, A, Dondini L, Del Duca, S, Vantini F, Bergamini C et al. (2004). A Zea mays 39-kDa thylakoid transglutaminase catalyses the modification by polyamines of light-harvesting complex II in a light-dependent way. Planta 219, 754–764.
- Dornemann D, Navakoudis E, Kotzabasis K (1996). Changes in the polyamine content of plastidal membranes in light- and dark-grown wild type and pigment mutants of the unicellular green alga Scenedesmus obliquus and their possible role in chloroplast photodevelopment. J Photochem Photobiol B: Biol 36, 293–299.
- Duan HG (1999). The effects of spermidine on nucleic acid and nuclease of the wheat in vitro blades in declining phase. J Sichuan Teachers Coll 20, 230–233 (in Chinese with an English abstract).
- Duan HG (2000). Effect of spermidine on protein contents and protease during senescence of excised wheat leaves. J Sichuan Teachers Coll 21, 44–47 (in Chinese with an English abstract).
- Hao LM, Wang HL, Liang HG (1999). Effects of rewatering on light-harvesting chlorophyll a/b protein complex of photosystem II in Zea mays. Acta Bot Sin 41, 613–616 (in Chinese with an English abstract).
- Havaux M (1992). Stress tolerance of photosystem II in vivo: Antagonistic effects of water, heat, and photoinhibition stresses. Plant Physiol 100, 424–432.
- He JX, An LZ, Lin HH, Liang HG (1999). Evidence for transcriptional and post-transcriptional control of protein synthesis in water-stressed wheat leaves: A quantitative analysis of messenger and ribosomal RNA. J Plant Physiol 155, 63–69.
- Hsiao TC (1973). Plant responses to water stress. Annu Rev Plant Physiol 24, 519–570.
- Igarashi K, Kashiwagi K (2000). Polyamines: Mysterious modulators of cellular functions. Biochem Biophys Res Commun 271, 559–564.
- Kasukabe Y, He L, Nada K, Misawa S, Ihara I, Tachibana S (2004). Overexpression of spermidine synthase enhances tolerance to multiple environmental stresses and up-regulates the expression of various stress-regulated genes in transgenic Arabidopsis thaliana. Plant Cell Physiol 45, 712–722.
- Konigshofer H, Lechner S (2002). Are polyamines involved in the synthesis of heat-shock proteins in cell suspension cultures of tobacco and alfalfa in response to high temperature stress Plant Physiol Biochem 40, 51–59.
- Kotzabasis K (1996). A role for chloroplast-associated polyamines Bot Acta 109, 5–7.
- Kotzabasis K, Senger H (1994). Free, conjugated and bound polyamines during the cell cycle in synchronized cultures of Scenedesmus obliquus. Z Naturforsch 49C, 181–189.
- Kotzabasis K, Fotinou C, Roubelakis-Angelakis KA, Ghanotakis D (1993). Polyamines in the photosynthetic apparatus. Photosystem II highly resolved subcomplexes are enriched in spermine. Photosynth Res 38, 83–88.
- Kotzabasis K, Navakoudis E, Tsolakis G, Senger H, Dornemann D (1999). Characterization of the photoreceptor(s) responsible for the regulation of the intracellular polyamine level and the putative participation of heterotrimeric G-proteins in the signal transduction chain. J Photochem Photobiol B: Biol 50, 38–44.
- Kumer A, Altabella T, Taylor MA, Tiburcio AF (1997). Recent advances in polyamine research. Trends Plant Sci 2, 124–130.
- Kuwabara T, Murata N (1982). Inactivation of photosynthetic oxygen evolution and concomitant release of three polypeptide in the photosystem II particles of spinach chloroplasts. Plant Cell Physiol 23, 533–539.
- Le Lay, P, Eullaffroy P, Juneau P, Popovic R (2000). Evidence of chlorophyll synthesis pathway alteration in desiccated barley leaves. Plant Cell Physiol 41, 565–570.
- Lichtenthaler HK, Wellburn AR (1983). Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Trans 11, 591–592.
- Liu HP, Dong BH, Zhang YY, Liu ZP, Liu YL (2004). Relationship between osmotic stress and the levels of free, conjugated and bound polyamines in leaves of wheat seedlings. Plant Sci 166, 1261–1267.
- Navakoudis E, Lutz C, Langebartelsc C, Lutz-Meindld U, Kotzabasis K (2003). Ozone impact on the photosynthetic apparatus and the protective role of polyamines. Biochim Biophys Acta 1621, 160–169.
- Paschalidis KA, Roubelakis-Angelakis KA (2005). Sites and regulation of polyamine catabolism in the tobacco plant. Correlations with cell division/expansion, cell cycle progression, and vascular development. Plant Physiol 138, 2174–2184.
- Pollard KJ, Samuels ML, Crowley KA, Hansen JC, Peterson CL (1999). Functional interaction between GCN5 and polyamines: A new role for core histone acetylation. EMBO J 18, 5622–5633.
- Roberts DR, Dumdroff EB, Thompson JE (1986). Exogenous polyamines alter membrane fluidity in bean leaves: A basis for potential misintepretation of their true physiological role. Planta 167, 395–401.
- Roy P, Niyogi K, SenGupta DN, Ghosh B (2005). Spermidine treatment to rice seedlings recovers salinity stress-induced damage of plasma membrane and PM-bound H+—ATPase in salt-tolerant and salt-sensitive rice cultivars. Plant Sci 168, 583–591.
- Sakurai I, Hagio M, Combos Z, Tyystjarvi T, Paakkarinen V, Aro E-M, Wada H (2003). Requirement of phosphatidylglycerol for maintenance of photosynthetic machinery. Plant Physiol 133, 1376–1384.
- Szalai G, Paldi E, Szigeti Z (1996). Role of light in the development of post-chilling symptoms in maize. J Plant Physiol 148, 378–383.
- Tang XS, Satoh K (1985). The oxygen-evolving photosystem II core complex. FEBS Lett 179, 60–64.
- Tang CF, Liu YG, Zeng GM, Li X, Xu WH, Li CF et al. (2005). Spermidine on antioxidant system responses of Typha latifolia L. under Cd2+ stress. J Integr Plant Biol 47, 428–434.
- Tiburcio AF, Campos JL, Figueras X, Besford RT (1993). Recent advances in the understanding of polyamines functions during plant development. Plant Growth Regul 12, 331–340.
- Tkachenko AG, Nesterova LY (2003). Polyamines as modulators of gene expression under oxidative stress in Escherichia coli. Biochemistry (Moscow) 68, 850–856.
- Tripathy BC, Mohanty P (1980). Zinc-inhibited electron transport of photosynthesis in isolated barley chloroplasts. Plant Physiol 66, 1174–1178.
- Velikova V, Yordanov I, Edreva A (2000). Oxidative stress and some antioxidant systems in acid rain-treated bean plants protective role of exogenous polyamines. Plant Sci 151, 59–66.
- Watanabe S, Kusama-Eguchi K, Kobayashi H, Igarashi K (1991). Estimation of polyamine binding to macromolecules and ATP in bovine lymphocytes and rat liver. J Biol Chem 266, 20803–20809.
- Yoshida M, Meksuriyen D, Kashiwagi K, Kawai G, Igarashi K (1999). Polyamine stimulation of the synthesis of oligopeptide-binding protein (OppA). Involvement of a structure change of the Shine-Dalgarno sequence and the initiation codon AUG in OppA mRNA. J Biol Chem 274, 22723–22728.
- Yuan S, Lin HH (2004). Transcription, translation, degradation and circadian clock. Biochem Biophys Res Commun 321, 1–6.
- Yuan S, Liu WJ, Zhang NH, Wang MB, Liang HG, Lin HH (2005). Effects of water stress on major photosystem II gene expression and protein metabolism in barley leaves. Physiol Plant 125, 464–473.
- Zhang NH, Wei ZQ, He JX, Du LF, Ling HG (2004). An efficient and economic method for preparation of high quality plant RNA. Prog Biochem Biophys 31, 947–950.
- Zhao FG, Sun C, Liu YL, Zhang WH (2003). Relationship between polyamine metabolism in roots and salt tolerance of barley seedlings. Acta Bot Sin 45, 295–300.
