Expression Analysis of Dihydroflavonol 4-Reductase Genes Involved in Anthocyanin Biosynthesis in Purple Grains of Wheat
Mao-Sen LIU
Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University , Taian, Shandong 271018, China
Search for more papers by this authorFang WANG
Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University , Taian, Shandong 271018, China
Search for more papers by this authorYu-Xiu DONG
Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University , Taian, Shandong 271018, China
Search for more papers by this authorCorresponding Author
Xian-Sheng ZHANG
Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University , Taian, Shandong 271018, China
*Author for correspondence. Tel: +86 (0)538 824 9418; Fax: +86 (0)538 822 6399; E-mail: 〈[email protected]〉.Search for more papers by this authorMao-Sen LIU
Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University , Taian, Shandong 271018, China
Search for more papers by this authorFang WANG
Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University , Taian, Shandong 271018, China
Search for more papers by this authorYu-Xiu DONG
Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University , Taian, Shandong 271018, China
Search for more papers by this authorCorresponding Author
Xian-Sheng ZHANG
Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University , Taian, Shandong 271018, China
*Author for correspondence. Tel: +86 (0)538 824 9418; Fax: +86 (0)538 822 6399; E-mail: 〈[email protected]〉.Search for more papers by this authorSupported by the National Special Program for Research and Industrialization of Transgenic Plants (JY-03-A-02); the State Key Basic Research and Development Plan of China (G19990116) and the Hi-Tech Research and Development (863) Program of China (2004AA212131).
Abstract
Abstract: The grain color of wheat (Triticum aestivum L.) is an important characteristic in crop production. Dihydroflavonol 4-reductase genes (DFR) encode the key enzyme dihydroflavonol 4-reductase, which is involved in the pigmentation of plant tissues. To investigate the molecular mechanism of anthocyanin deposition in grains of wheat, we determined the expression of the wheat DFR gene in purple grains of cultivar Heimai 76. The results showed that DFR transcripts were localized in the seed coat of purple grains rather than in the pericarp, whereas anthocyanins were accumulated in both tissues of purple grains, suggesting that anthocyanin deposition was mainly regulated at the transcriptional level. Overexpression of the TaDFR-A gene in Arabidopsis showed that TaDFR-A was responsible for the pigmentation of Arabidopsis plant tissues, indicating TaDFR-A gene has the same role in Arabidopsis.
(Managing editor: Li-Hui ZHAO)
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