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LOCALIZATION OF ANTIGENS IN THIN SECTIONS OF BACTERIA BY THE IMMUNO-PEROXIDASE TECHNIQUE
A. HAUGEN,
S. HELGELAND,
A. GROV,
Corresponding Author
A. HAUGEN
The University of Bergen, School of Medicine, The Gade Institute, Department of Microbiology, Bergen, Norway
University of Bergen, School of Medicine, The Gade Institute, Department of Microbiology, Bergen, Norway.Search for more papers by this authorS. HELGELAND
The University of Bergen, School of Medicine, The Gade Institute, Department of Microbiology, Bergen, Norway
Search for more papers by this authorA. GROV
The University of Bergen, School of Medicine, The Gade Institute, Department of Microbiology, Bergen, Norway
Search for more papers by this authorA. HAUGEN,
S. HELGELAND,
A. GROV,
Corresponding Author
A. HAUGEN
The University of Bergen, School of Medicine, The Gade Institute, Department of Microbiology, Bergen, Norway
University of Bergen, School of Medicine, The Gade Institute, Department of Microbiology, Bergen, Norway.Search for more papers by this authorS. HELGELAND
The University of Bergen, School of Medicine, The Gade Institute, Department of Microbiology, Bergen, Norway
Search for more papers by this authorA. GROV
The University of Bergen, School of Medicine, The Gade Institute, Department of Microbiology, Bergen, Norway
Search for more papers by this authorFirst published: September 1975
Abstract
The immunoperoxidase technique together with electron microscopy has been examined for the ultrastructural localization of staphylococcal protein A, the immunoferritin method being included for comparison. The results show that protein A is uniformly distributed in the whole cell wall. Both the direct and indirect methods, Fab- as well as Fc-reactions, showed identical results. The immunoperoxidase method was superior to the immunoferritin method, especially when applied to thin sections of the bacteria, and the clear specificity demonstrated indicates a useful method for localization of cellular antigens.
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