Triple swabbing allows sensitive MRSA detection in dermatologic patients of a university tertiary care hospital
Corresponding Author
Georg Daeschlein
Department of Dermatology of the Ernst Moritz Arndt University, Greifswald, Germany
Conflicts of interest None.
Correspondence to
Priv.-Doz. Dr. med. habil. Georg Daeschlein
Department of Dermatology of the Ernst Moritz Arndt University Greifswald
Sauerbruchstrafle
17489 Greifswald, Germany
E-mail: [email protected]
Search for more papers by this authorTina Bloom
Department of Dermatology of the Ernst Moritz Arndt University, Greifswald, Germany
Conflicts of interest None.
Search for more papers by this authorSebastian von Podewils
Department of Dermatology of the Ernst Moritz Arndt University, Greifswald, Germany
Conflicts of interest None.
Search for more papers by this authorOjan Assadian
Clinical Institute for Hospital Hygiene, Medical University of Vienna, Vienna, Austria
Search for more papers by this authorJohann HT Wagenvoort
Department of Medical Microbiology, Atrium Medical Centre Parkstad, Heerlen, The Netherlands
Search for more papers by this authorHelene Riebe
Department of Dermatology of the Ernst Moritz Arndt University, Greifswald, Germany
Search for more papers by this authorSebastian Fochler
Department of Dermatology of the Ernst Moritz Arndt University, Greifswald, Germany
Search for more papers by this authorAbdulkareem Al-Jebori
Department of Dermatology of the Ernst Moritz Arndt University, Greifswald, Germany
Search for more papers by this authorSyrus Karsai
Department of Dermatology of the Ernst Moritz Arndt University, Greifswald, Germany
Department of Dermatology, Darmstadt Hospital, Darmstadt, Germany
Search for more papers by this authorEllen Kaisermayer
Department of Dermatology of the Ernst Moritz Arndt University, Greifswald, Germany
Search for more papers by this authorHermann Haase
Department of Dermatology of the Ernst Moritz Arndt University, Greifswald, Germany
Search for more papers by this authorSebastian Scholz
Department of Dermatology of the Ernst Moritz Arndt University, Greifswald, Germany
Search for more papers by this authorMichael Jünger
Department of Dermatology of the Ernst Moritz Arndt University, Greifswald, Germany
Search for more papers by this authorCorresponding Author
Georg Daeschlein
Department of Dermatology of the Ernst Moritz Arndt University, Greifswald, Germany
Conflicts of interest None.
Correspondence to
Priv.-Doz. Dr. med. habil. Georg Daeschlein
Department of Dermatology of the Ernst Moritz Arndt University Greifswald
Sauerbruchstrafle
17489 Greifswald, Germany
E-mail: [email protected]
Search for more papers by this authorTina Bloom
Department of Dermatology of the Ernst Moritz Arndt University, Greifswald, Germany
Conflicts of interest None.
Search for more papers by this authorSebastian von Podewils
Department of Dermatology of the Ernst Moritz Arndt University, Greifswald, Germany
Conflicts of interest None.
Search for more papers by this authorOjan Assadian
Clinical Institute for Hospital Hygiene, Medical University of Vienna, Vienna, Austria
Search for more papers by this authorJohann HT Wagenvoort
Department of Medical Microbiology, Atrium Medical Centre Parkstad, Heerlen, The Netherlands
Search for more papers by this authorHelene Riebe
Department of Dermatology of the Ernst Moritz Arndt University, Greifswald, Germany
Search for more papers by this authorSebastian Fochler
Department of Dermatology of the Ernst Moritz Arndt University, Greifswald, Germany
Search for more papers by this authorAbdulkareem Al-Jebori
Department of Dermatology of the Ernst Moritz Arndt University, Greifswald, Germany
Search for more papers by this authorSyrus Karsai
Department of Dermatology of the Ernst Moritz Arndt University, Greifswald, Germany
Department of Dermatology, Darmstadt Hospital, Darmstadt, Germany
Search for more papers by this authorEllen Kaisermayer
Department of Dermatology of the Ernst Moritz Arndt University, Greifswald, Germany
Search for more papers by this authorHermann Haase
Department of Dermatology of the Ernst Moritz Arndt University, Greifswald, Germany
Search for more papers by this authorSebastian Scholz
Department of Dermatology of the Ernst Moritz Arndt University, Greifswald, Germany
Search for more papers by this authorMichael Jünger
Department of Dermatology of the Ernst Moritz Arndt University, Greifswald, Germany
Search for more papers by this authorequal participation.
Summary
Background
To optimize preventive measures to control MRSA, we investigated retrospectively the suitability of a multiple site screening model and the optimal sampling technique to detect MRSA in a university-based phlebology and skin cancer center in Germany.
Patients and Methods
During 4.5 years samples of 3 712 inpatients in a dermatologic department were analyzed for MRSA by conventional microbiologic cultures and in parallel by PCR. Samples were taken from nares, wounds and skin lesions.
Results
MRSA was detected in 60 inpatients (1.6%). 268 of 7 269 (3.7%) samples at admission and during hospital stay were found positive ñ 96 (35.8%) of these were swabs of nares, 59 (22.0%) surveillance swabs, 53 (19.8%) wound swabs and 42 (15.7%) from other dermatologic lesions. Twenty-five of 60 patients (41.7%) were found positive only in the nares, 10 (16.7%) patients only in wounds and 4 (6.7%) patients only in lesions. 166 (61.9%) of all positive culture samples became positive 24 hours after cultivation, 86 (32.1%) after 48 hours, and 16 (6.0%) after 72 hours.
Conclusions
Highest sensitivity to detect MRSA can be reached by combining three swabs: nares, wounds and skin lesions (ìtriple-testî). Culture of screening specimens for 72 hours is recommended.
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