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Peroxidases, Iactoferrin and lysozyme in peripheral blood neutrophils, gingival crevicular fluid and whole saliva of patients with localized juvenile periodontitis

Corresponding Author

K Suomalainen

Department of Periodontology. Institute of Dentistry, University of Helsinki, Helsinki

*Institute of Dentistry, Department of Periodontology, PO Box 41, Mannerheimintie 172, FIN-00014 University of Helsinki, FinlandSearch for more papers by this author

L Saxén,

L Saxén

Department of Periodontology. Institute of Dentistry, University of Helsinki, Helsinki

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P Vilja,

P Vilja

Medical School, University of Tampere, Tampere

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J Tenovuo,

J Tenovuo

Turku Immunology Centre and Department of Cariology, Institute of Dentistry, University of Turkir, Turku. Finland

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K Suomalainen,

Corresponding Author

K Suomalainen

Department of Periodontology. Institute of Dentistry, University of Helsinki, Helsinki

*Institute of Dentistry, Department of Periodontology, PO Box 41, Mannerheimintie 172, FIN-00014 University of Helsinki, FinlandSearch for more papers by this author

L Saxén,

L Saxén

Department of Periodontology. Institute of Dentistry, University of Helsinki, Helsinki

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P Vilja,

P Vilja

Medical School, University of Tampere, Tampere

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J Tenovuo,

J Tenovuo

Turku Immunology Centre and Department of Cariology, Institute of Dentistry, University of Turkir, Turku. Finland

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First published: June 1996

https://doi.org/10.1111/j.1601-0825.1996.tb00213.x

Citations: 22

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Abstract

OBJECTIVE: The aim of this study was to examine the longitudinal association of selected non-immune antimicrobial host factors (peroxidases, lysozyme and lactoferrin) to the localized juvenile periodontitis (LJP) disease status.

MATERIALS AND METHODS: Peroxidases, lysozyme and lactoferrin were quantitated from seven patients with LJP before and after periodontal therapy. Analyses were performed from simultaneously collected samples of peripheral blood polymorphonuclear leukocytes (PMNs), gingival crevicular fluid (GCF from diseased sites) and paraffin-stimulated whole saliva. Similar assays were done also from seven periodontally healthy controls.

RESULTS During untreated phase of LIP myeloperoxid-ase, lysozyme and lactoferrin concentrations were remarkably elevated in peripheral blood PMNs, also reflected in their high concentrations in GCF. All these values normalised with respect to healthy controls during the periodontal therapy. No similar longitudinal changes were seen in whole saliva but during therapy salivary per-oxidase concentrations declined below the control values, in accordance with our previous observations in parotid saliva samples of LJP patients.

CONCLUSIONS: In LJP the concentrations of lysozyme, lactoferrin and myeloperoxidase are significantly elevated in peripheral blood PMNs, also reflected in GCF. During periodontal therapy these values decline and approach those observed in healthy controls. No similar changes are seen in stimulated whole saliva.

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Volume2, Issue2

June 1996

Pages 129-134

Peroxidases, Iactoferrin and lysozyme in peripheral blood neutrophils, gingival crevicular fluid and whole saliva of patients with localized juvenile periodontitis

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Peroxidases, Iactoferrin and lysozyme in peripheral blood neutrophils, gingival crevicular fluid and whole saliva of patients with localized juvenile periodontitis

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