Gene Expression Profiling of the Rat Endometriosis Model
Ryo Konno
Department of Gynecology, Omiya Medical Center Jichi Medical University, Amanuma-cho, Omiya, Saitama, Japan;
Search for more papers by this authorHiroyuki Fujiwara
Department of Gynecology, Omiya Medical Center Jichi Medical University, Amanuma-cho, Omiya, Saitama, Japan;
Search for more papers by this authorSachiho Netsu
Department of Gynecology, Omiya Medical Center Jichi Medical University, Amanuma-cho, Omiya, Saitama, Japan;
Search for more papers by this authorKohei Odagiri
Department of Gynecology, Omiya Medical Center Jichi Medical University, Amanuma-cho, Omiya, Saitama, Japan;
Search for more papers by this authorMiyuki Shimane
Laboratory of System Biology and Medicine, Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo, Japan;
Search for more papers by this authorHitoshi Nomura
Laboratory of System Biology and Medicine, Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo, Japan;
Search for more papers by this authorMitsuaki Suzuki
Department of Obstetrics and Gynecology, Jichi Medical University, Shimotuske, Tochigi, Japan
Search for more papers by this authorRyo Konno
Department of Gynecology, Omiya Medical Center Jichi Medical University, Amanuma-cho, Omiya, Saitama, Japan;
Search for more papers by this authorHiroyuki Fujiwara
Department of Gynecology, Omiya Medical Center Jichi Medical University, Amanuma-cho, Omiya, Saitama, Japan;
Search for more papers by this authorSachiho Netsu
Department of Gynecology, Omiya Medical Center Jichi Medical University, Amanuma-cho, Omiya, Saitama, Japan;
Search for more papers by this authorKohei Odagiri
Department of Gynecology, Omiya Medical Center Jichi Medical University, Amanuma-cho, Omiya, Saitama, Japan;
Search for more papers by this authorMiyuki Shimane
Laboratory of System Biology and Medicine, Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo, Japan;
Search for more papers by this authorHitoshi Nomura
Laboratory of System Biology and Medicine, Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo, Japan;
Search for more papers by this authorMitsuaki Suzuki
Department of Obstetrics and Gynecology, Jichi Medical University, Shimotuske, Tochigi, Japan
Search for more papers by this authorAbstract
Problem
To investigate the molecular mechanism of endometriosis, gene expression profiling was analyzed in a rat endometriosis model.
Method of study
An endometriosis model was induced by uterine autotransplantation in the peritoneal cavity on a female-SD rat (8 weeks old). As control samples, the normal uterine tissues were used. The gene expression was compared between endometriotic lesions and normal uterine tissues by cDNA microarray analysis, quantitative real time RT-PCR and immunohistochemistry.
Results
The expression of 71 genes was upregulated and that of 45 genes was downregulated in the endometriotic lesions compared to normal uterine tissues. The upregulated genes included genes encoding cytokines, chemokines, growth factors and cell adhesion molecules. The levels of transcripts of osteopontin, Lyn, Vav1, Runx1, and l-selectin in the endometriotic lesions were 130, 10, 10, 12 and 46-fold higher than the respective levels in the eutopic endometrial samples.
Conclusion
The results suggest that osteopontin, Lyn, Vav1, Runx1, and l-selectin play important roles in the pathogenesis of endometriosis.
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