PROBLEM: Thymectomy of mice on day 3 after birth (3d-Tx) gives rise to the appearance of a particular type of ovarian lesion known as experimental autoimmune oophoritis (AIO).

METHOD: In the present study, the spleen cells from BALB/c mice having undergone 3 day-Tx AIO were fused with X63-Ag8-6.5.3 myeloma cells, and a number of hybridoma clones producing autoantibodies against ovarian tissue were established.

RESULTS: On the basis of immunohistochemical and protein-chemical analysis, two independent clones, designated as T2.2 and S1.6, were found to react with interstitial tissues surrounding the follicles. These monoclonal antibodies showed broad cross-species reactivity, in that they recognized similar antigenic macromolecules in the rat, pig, human, and mouse. The antigenic determinants were strongly resistant to heat and acid, especially to the treatment of periodic acid, indicating that the antigenic determinants had no relation with carbohydrate components.

CONCLUSIONS: By antibody affinity chromotography, two kinds of autoantigens were identified. SDS-PAGE, under reduced or nonreduced conditions, revealed an 80/85 kDa protein for T2.2, and an 82 kDa for S1.6, respectively.

REFERENCES

1 Shoenfeld Y, Isenberg D. The mosaic of autoimmunity (the factors associated with autoimmune disease): Autoimmune diseases—clinical features and animal models. Res Monogr Immunol. 1989; 12: 13–74.
Google Scholar
2 Nishizuka Y, Sakakura T. Thymus and reproduction: Sex-linked dysgenesis of the gonad after neonatal thymectomy in mice. Science. 1969; 166: 753–755.
10.1126/science.166.3906.753
CAS PubMed Web of Science® Google Scholar
3 Tung KSK, Smith S, Feuchter F, Goldstein G, Cook C, Anderson RE. Murine autoimmune oophoritis, epididymoorchitis, and gastritis induced by day 3 thymectomy. Am J Pathol. 1987; 126: 293–302.
CAS PubMed Web of Science® Google Scholar
4 Zamvil SS, Steinman L. The T lymphocyte in experimental allergic encephalomyelitis. Ann Rev Immunol. 1990; 8: 579–621.
10.1146/annurev.iy.08.040190.003051
CAS PubMed Web of Science® Google Scholar
5 Mori Y, Fukuma K, Adachi Y, Shigeta K, Kannagi R, Tanaka H, Sakai M, Kuribayashi K, Uchino H, Masuda T. Parietal cell autoantigens involved in neonatal thymectomy-induced murine autoimmune gastritis. Studies using monoclonal autoantibodies. Gastroenterology. 1989; 97: 364–375.
10.1016/0016-5085(89)90072-3
CAS PubMed Web of Science® Google Scholar
6 Sakaguchi S, Fukuma K, Kuribayashi K, Masuda T. Organ-specific autoimmune diseases induced in mice by elimination of T cell subset. I. Evidence for the active participation of T cells in natural self-tolerance; deficit of a T cell subset as a possible cause of autoimmune disease. J Exp Med. 1985; 161: 72–87.
10.1084/jem.161.1.72
CAS PubMed Web of Science® Google Scholar
7 Fukuma M, Sakaguchi S, Kuribayashi K, Chen WL, Morishita R, Sekita K, Uchino H, Masuda T. Immunologic and clinical studies on murine autoimmune gastritis induced by neonatal thymectomy. Gastroenterology. 1988; 94: 274–283.
10.1016/0016-5085(88)90413-1
CAS PubMed Web of Science® Google Scholar
8 Toh B-H, Gleeson PA, Simpson RJ, Moritz RL, Callaghan JM, Goldkom I, Jones CM, Martinelli TM, Mu F-T, Humphris DC, Pettitt JM, Mori Y, Masuda T, Soboeszczuk P, Weinstock J, Mantamadiotis T, Baldwin GS. The 60-to 90-kDa parietal cell autoantigen associated with autoimmune gastritis is a β subunit of the gastric H⁺/K⁺-ATPase (proton pump). Proc Natl Acad Sci USA. 1990; 87: 6418–6422.
10.1073/pnas.87.16.6418
CAS PubMed Web of Science® Google Scholar
9 Jones CM, Callaghan JM, Gleeson PA, Mori Y, Masuda T, Toh B-H. The parietal cell autoantibodies recognized in neonatal thymectomy-induced murine gastritis are the α and β subunits of the gastric proton pump. Gastroenterology. 1991; 101: 287–294.
10.1016/0016-5085(91)90002-3
CAS PubMed Web of Science® Google Scholar
10 Cohen I, Speroff L. Premature ovarian failure: Update. Obstet Gynecol Survey. 1991; 46: 156–162.
10.1097/00006254-199103000-00015
CAS PubMed Google Scholar
11 Irvine WJ, Bornes EW. Addison's disease, ovarian failure and hypoparathyroidism. Clin Endocrinol (Oxf). 1975; 4: 379–388.
10.1016/S0300-595X(75)80027-2
Web of Science® Google Scholar
12 Vasquez AM, Kenny FM. Ovarian failure and antiovarian antibodies in association with hypoparathyroidism, moniliasis and Addison's and Hashimoto's disease. Obstet Gynecol. 1973; 41: 414–418.
PubMed Google Scholar
13 Miyake T, Taguchi O, Ikeda H, Sato Y, Takeuchi S, Nishizuka Y. Acute oocyte loss in experimental autoimmune oophoritis as a possible model of premature ovarian failure. Am J Obstet Gynecol. 1988; 158: 186–192.
10.1016/0002-9378(88)90808-3
CAS PubMed Web of Science® Google Scholar
14 Littlefield JW. Selection of hybrids from matings of fibroblasts in vitro and their presumed recombinants. Science. 1964; 147: 709–710.
10.1126/science.145.3633.709
Web of Science® Google Scholar
15 Hawkes R, Nidey E, Gordon J. A dot-immunebinding assay for monoclonal and other antibodies. Anal Biochem. 1982; 119: 142–147.
10.1016/0003-2697(82)90677-7
PubMed Web of Science® Google Scholar
16 Cuatrecasas P, Wicheck M, Anfinsen CB. Selective enzyme purification by affinity chromatography. Proc Natl Acad Sci USA. 1968; 61: 636–643.
10.1073/pnas.61.2.636
CAS PubMed Web of Science® Google Scholar
17 Magoffin DA, Kurtz KM, Erickson GF Insulin-like growth factor-1 selectively stimulates cholesterol side-chain cleavage expression in ovarian theca-interstitial cells. Mol. Endocrinol. 1990; 4: 489–496.
10.1210/mend-4-3-489
CAS PubMed Web of Science® Google Scholar
18 Bleil JD, Wassarman PM. Mammalian sperm-egg interaction: Identification of a glycoprotein in mouse egg zonae pellucidae possessing receptor activity for sperm. Cell. 1980; 20: 873–882.
10.1016/0092-8674(80)90334-7
CAS PubMed Web of Science® Google Scholar
19 Wassarman PM. The biology and chemistry of fertilization. Science. 1987; 235: 553.
10.1126/science.3027891
CAS PubMed Web of Science® Google Scholar
20 Collen RJ, Lippe BM, Kaplan SA. Primary ovarian failure. Juvenile rheumatoid arthritis and vitiligo. Am J Dis Child. 1979; 133: 598–600.
CAS PubMed Web of Science® Google Scholar
21 Williamson HO, Phnsey SA, Mathur S, Mathur RS, Baker ER, Fudenberg HH. Myasthenia gravis, premature menopause and thyroid autoimmunity. Am J Obstet Gynecol. 1980; 137: 893–901.
CAS PubMed Web of Science® Google Scholar

Volume29, Issue4

May 1993

Pages 211-218

Interspecies-specific Ovarian Autoantigens Involved in Neonatal Thymectomy-induced Murine Autoimmune Oophoritis

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Interspecies-specific Ovarian Autoantigens Involved in Neonatal Thymectomy-induced Murine Autoimmune Oophoritis

Abstract

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References

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