NGF and its receptors TrkA and p75NTR in the epithelium of oral lichen
Katsuhiko Hayashi
Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway
Department of Dentistry, Jikei University School of Medicine, Tokyo, Japan
Search for more papers by this authorAndreas Karatsaidis
Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway
Department of Oral Surgery and Oral Medicine, University of Bergen, Bergen, Norway
Search for more papers by this authorOlav Schreurs
Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway
Search for more papers by this authorTore Bjørnland
Department of Oral Surgery and Oral Medicine, Faculty of Dentistry, University of Oslo, Oslo, Norway
Search for more papers by this authorMasashi Sugisaki
Department of Dentistry, Jikei University School of Medicine, Tokyo, Japan
Search for more papers by this authorKarl Schenck
Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway
Search for more papers by this authorKatsuhiko Hayashi
Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway
Department of Dentistry, Jikei University School of Medicine, Tokyo, Japan
Search for more papers by this authorAndreas Karatsaidis
Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway
Department of Oral Surgery and Oral Medicine, University of Bergen, Bergen, Norway
Search for more papers by this authorOlav Schreurs
Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway
Search for more papers by this authorTore Bjørnland
Department of Oral Surgery and Oral Medicine, Faculty of Dentistry, University of Oslo, Oslo, Norway
Search for more papers by this authorMasashi Sugisaki
Department of Dentistry, Jikei University School of Medicine, Tokyo, Japan
Search for more papers by this authorKarl Schenck
Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway
Search for more papers by this authorAbstract
Background: Nerve growth factor (NGF) can through its receptors TrkA and p75NTR convey signals for cell survival, differentiation and death. The aim of this study was to examine whether NGF can play a role in the pathology of oral lichen (OL).
Methods: Sections from biopsies taken from patients with erythematous (ERY) OL and from volunteers with normal oral mucosa (NOM) were immunostained with antibodies against NGF, proNGF, TrkA, phosphorylated Trk, p75NTR and phosphorylated Akt (pAkt) and expression of RNA coding for proNGF/NGF was investigated by in situ hybridization.
Results: Both in ERY OL and NOM, cytoplasmic staining for NGF was seen in granular and upper spinous cell layers of the epithelium, whereas proNGF staining was seen in all epithelial cell layers. In situ hybridization showed that the proNGF protein was produced in the same cell layers. In OL, strong cytoplasmic stainings for TrkA and activated Trk (pTrk) were observed in all epithelial cell layers while these stainings were only weak in NOM. Basal keratinocytes in OL showed no or only weak cytoplasmic staining for p75NTR, but in NOM there was a clear cell membrane staining. In OL, strong cytoplasmic and intermittent nuclear staining for pAkt was observed in spinous, granular and superficial layers, while basal and parabasal keratinocytes were negative. This staining was weak or absent in the entire epithelium of NOM.
Conclusions: TrkA upregulation and activation in OL is one of the pathways that can activate pAkt and thereby rescue epithelial cells from untimely cell death.
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