An altered keratinocyte phenotype in oral submucous fibrosis: correlation of keratin K17 expression with disease severity
Anand Lalli
Programme in Muco-Cutaneous Oncology, Centre for Clinical and Diagnostic Oral Sciences, Institute of Cell and Molecular Sciences, London, UK
Search for more papers by this authorWanninayake M. Tilakaratne
Programme in Muco-Cutaneous Oncology, Centre for Clinical and Diagnostic Oral Sciences, Institute of Cell and Molecular Sciences, London, UK
Department of Oral Pathology, University of Peradeniya, Peradeniya, Sri Lanka
Search for more papers by this authorAnura Ariyawardana
Department of Oral Medicine and Periodontology, Faculty of Dental Sciences, University of Peradeniya, Peradeniya, Sri Lanka
Search for more papers by this authorCaroline Fitchett
Programme in Muco-Cutaneous Oncology, Centre for Clinical and Diagnostic Oral Sciences, Institute of Cell and Molecular Sciences, London, UK
Search for more papers by this authorIrene M. Leigh
Centre for Cutaneous Research; Barts and The London, Queen Mary’s School of Medicine and Dentistry, Institute of Cell and Molecular Sciences, London, UK
Present address: College of Medicine, Dentistry and Nursing, University of Dundee, Level 10, Ninewells Hospital & Medical School, Dundee DD1 9SY, UK.
Search for more papers by this authorEleni Hagi-Pavli
Programme in Muco-Cutaneous Oncology, Centre for Clinical and Diagnostic Oral Sciences, Institute of Cell and Molecular Sciences, London, UK
Search for more papers by this authorAlan T. Cruchley
Programme in Muco-Cutaneous Oncology, Centre for Clinical and Diagnostic Oral Sciences, Institute of Cell and Molecular Sciences, London, UK
Search for more papers by this authorE. Kenneth Parkinson
Programme in Muco-Cutaneous Oncology, Centre for Clinical and Diagnostic Oral Sciences, Institute of Cell and Molecular Sciences, London, UK
Search for more papers by this authorMuy-Teck Teh
Programme in Muco-Cutaneous Oncology, Centre for Clinical and Diagnostic Oral Sciences, Institute of Cell and Molecular Sciences, London, UK
Search for more papers by this authorFarida Fortune
Programme in Muco-Cutaneous Oncology, Centre for Clinical and Diagnostic Oral Sciences, Institute of Cell and Molecular Sciences, London, UK
Search for more papers by this authorAhmad Waseem
Programme in Muco-Cutaneous Oncology, Centre for Clinical and Diagnostic Oral Sciences, Institute of Cell and Molecular Sciences, London, UK
Search for more papers by this authorAnand Lalli
Programme in Muco-Cutaneous Oncology, Centre for Clinical and Diagnostic Oral Sciences, Institute of Cell and Molecular Sciences, London, UK
Search for more papers by this authorWanninayake M. Tilakaratne
Programme in Muco-Cutaneous Oncology, Centre for Clinical and Diagnostic Oral Sciences, Institute of Cell and Molecular Sciences, London, UK
Department of Oral Pathology, University of Peradeniya, Peradeniya, Sri Lanka
Search for more papers by this authorAnura Ariyawardana
Department of Oral Medicine and Periodontology, Faculty of Dental Sciences, University of Peradeniya, Peradeniya, Sri Lanka
Search for more papers by this authorCaroline Fitchett
Programme in Muco-Cutaneous Oncology, Centre for Clinical and Diagnostic Oral Sciences, Institute of Cell and Molecular Sciences, London, UK
Search for more papers by this authorIrene M. Leigh
Centre for Cutaneous Research; Barts and The London, Queen Mary’s School of Medicine and Dentistry, Institute of Cell and Molecular Sciences, London, UK
Present address: College of Medicine, Dentistry and Nursing, University of Dundee, Level 10, Ninewells Hospital & Medical School, Dundee DD1 9SY, UK.
Search for more papers by this authorEleni Hagi-Pavli
Programme in Muco-Cutaneous Oncology, Centre for Clinical and Diagnostic Oral Sciences, Institute of Cell and Molecular Sciences, London, UK
Search for more papers by this authorAlan T. Cruchley
Programme in Muco-Cutaneous Oncology, Centre for Clinical and Diagnostic Oral Sciences, Institute of Cell and Molecular Sciences, London, UK
Search for more papers by this authorE. Kenneth Parkinson
Programme in Muco-Cutaneous Oncology, Centre for Clinical and Diagnostic Oral Sciences, Institute of Cell and Molecular Sciences, London, UK
Search for more papers by this authorMuy-Teck Teh
Programme in Muco-Cutaneous Oncology, Centre for Clinical and Diagnostic Oral Sciences, Institute of Cell and Molecular Sciences, London, UK
Search for more papers by this authorFarida Fortune
Programme in Muco-Cutaneous Oncology, Centre for Clinical and Diagnostic Oral Sciences, Institute of Cell and Molecular Sciences, London, UK
Search for more papers by this authorAhmad Waseem
Programme in Muco-Cutaneous Oncology, Centre for Clinical and Diagnostic Oral Sciences, Institute of Cell and Molecular Sciences, London, UK
Search for more papers by this authorAbstract
Oral submucous fibrosis (OSF) is characterized by abnormal collagen metabolism in the submucosal connective tissue. Its influence on the overlying epithelium is not known but about 14% of OSF cases undergo malignant transformation to squamous cell carcinoma indicating association with abnormality of the epithelium. Here, we have defined the keratin expression profile, by immunohistochemistry and quantitative image analysis, using a panel of 22 anti-keratin monoclonal antibodies on 28 OSF samples. We observed an increase of K1 and K10 in the suprabasal layers, induction of K6 in the basal layer and complete loss of K19 in the epithelium. Furthermore, there was increased K17 expression in the suprabasal layers, which correlated with disease severity. In a subset of the most severe OSF cases (14%), K17 expression was completely lost in the basal layer which might define them to be at most risk to undergo malignant transformation. There was no detectable expression of K8, K18, K7 and K9 and the expression of K4, K13, K14, K15 and K16 did not change in OSF. We propose that the altered keratin profiles could be useful as histological diagnostic markers and provide important insights into the pathogenesis of the disease and its predisposition to malignancy.
Supporting Information
Table S1: Keratin immunostaining of OSF and normal epithelial samples: correlation between visual assessment and pixel analysis
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