Volume 18, Issue 4 pp. 362-369

Promotion of incisional wound repair by human mesenchymal stem cell transplantation

Alexander Stoff

Alexander Stoff

Division of Human Gene Therapy, Departments of Medicine, Obstetrics and Gynecology, Pathology, Surgery, and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL, USA

Department of Plastic and Reconstructive Surgery, Dreifaltigkeits-Hospital, Wesseling, Germany

Department of Plastic and Reconstructive Surgery, University of Alabama at Birmingham, Birmingham, AL, USA

Search for more papers by this author
Angel A. Rivera

Angel A. Rivera

Division of Human Gene Therapy, Departments of Medicine, Obstetrics and Gynecology, Pathology, Surgery, and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL, USA

Search for more papers by this author
N. Sanjib Banerjee

N. Sanjib Banerjee

Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL, USA

Search for more papers by this author
Steven T. Moore

Steven T. Moore

Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL, USA

Search for more papers by this author
T. Michael Numnum

T. Michael Numnum

Division of Human Gene Therapy, Departments of Medicine, Obstetrics and Gynecology, Pathology, Surgery, and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL, USA

Search for more papers by this author
Antonio Espinosa-de-los-Monteros

Antonio Espinosa-de-los-Monteros

Department of Plastic and Reconstructive Surgery, Dreifaltigkeits-Hospital, Wesseling, Germany

Search for more papers by this author
Dirk F. Richter

Dirk F. Richter

Department of Plastic and Reconstructive Surgery, Dreifaltigkeits-Hospital, Wesseling, Germany

Search for more papers by this author
Gene P. Siegal

Gene P. Siegal

Division of Human Gene Therapy, Departments of Medicine, Obstetrics and Gynecology, Pathology, Surgery, and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL, USA

Search for more papers by this author
Louise T. Chow

Louise T. Chow

Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL, USA

Search for more papers by this author
Dale Feldman

Dale Feldman

Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL, USA

Search for more papers by this author
Luis O. Vasconez

Luis O. Vasconez

Department of Plastic and Reconstructive Surgery, University of Alabama at Birmingham, Birmingham, AL, USA

Search for more papers by this author
J. Michael Mathis

J. Michael Mathis

Gene Therapy Program, Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, Shreveport, LA, USA

Search for more papers by this author
Mariam A. Stoff-Khalili

Mariam A. Stoff-Khalili

Division of Human Gene Therapy, Departments of Medicine, Obstetrics and Gynecology, Pathology, Surgery, and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL, USA

Department of Gynecology and Obstetrics, University of Duesseldorf, Medical Center, Duesseldorf, Germany

Search for more papers by this author
David T. Curiel

David T. Curiel

Division of Human Gene Therapy, Departments of Medicine, Obstetrics and Gynecology, Pathology, Surgery, and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL, USA

Search for more papers by this author
First published: 18 March 2009
Citations: 101
David T. Curiel, M.D., Ph.D., Director, Division of Human Gene Therapy, Gene Therapy Center, University of Alabama at Birmingham, 901 19th Street South, BMR2 502, Birmingham, AL 35294, USA, Tel.: 205 934 8627, Fax: 205 975 7476, e-mail: [email protected]

Abstract

Abstract: The purpose of this study was to determine the effect of transplanted human mesenchymal stem cells (hMSCs) on wound healing. In this model, full-thickness cutaneous wounds were created by incision in the skin of adult New Zealand white rabbits and treated by transplanted hMSCs into the wounds. Wound healing was evaluated by histological analysis and tensiometry over time. A total of 15 New Zealand white rabbits with 10 wounds per animal were examined in this study. Animals were treated with hMSCs and euthanised at 3, 7, 14, 21 and 80 days after manipulation. The hMSCs were labelled with a fluorescent dye (CM-DiI), suspended in phosphate-buffered saline and used to treat full-thickness incisional wounds in rabbit skin. Tensiometry and histology were used to characterise the wound-healing rate of the incisional wounds. These results showed that transplanted hMSCs significantly inhibited scar formation and increased the tensile strength of the wounds. Importantly, MSCs from genetically unrelated donors did not appear to induce an immunologic response. In conclusion, human mesenchymal stem cell therapy is a viable approach to significantly affect the course of normal cutaneous wound healing and significantly increase the tensile strength.

The full text of this article hosted at iucr.org is unavailable due to technical difficulties.