Promotion of incisional wound repair by human mesenchymal stem cell transplantation
Alexander Stoff
Division of Human Gene Therapy, Departments of Medicine, Obstetrics and Gynecology, Pathology, Surgery, and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL, USA
Department of Plastic and Reconstructive Surgery, Dreifaltigkeits-Hospital, Wesseling, Germany
Department of Plastic and Reconstructive Surgery, University of Alabama at Birmingham, Birmingham, AL, USA
Search for more papers by this authorAngel A. Rivera
Division of Human Gene Therapy, Departments of Medicine, Obstetrics and Gynecology, Pathology, Surgery, and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL, USA
Search for more papers by this authorN. Sanjib Banerjee
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL, USA
Search for more papers by this authorSteven T. Moore
Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL, USA
Search for more papers by this authorT. Michael Numnum
Division of Human Gene Therapy, Departments of Medicine, Obstetrics and Gynecology, Pathology, Surgery, and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL, USA
Search for more papers by this authorAntonio Espinosa-de-los-Monteros
Department of Plastic and Reconstructive Surgery, Dreifaltigkeits-Hospital, Wesseling, Germany
Search for more papers by this authorDirk F. Richter
Department of Plastic and Reconstructive Surgery, Dreifaltigkeits-Hospital, Wesseling, Germany
Search for more papers by this authorGene P. Siegal
Division of Human Gene Therapy, Departments of Medicine, Obstetrics and Gynecology, Pathology, Surgery, and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL, USA
Search for more papers by this authorLouise T. Chow
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL, USA
Search for more papers by this authorDale Feldman
Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL, USA
Search for more papers by this authorLuis O. Vasconez
Department of Plastic and Reconstructive Surgery, University of Alabama at Birmingham, Birmingham, AL, USA
Search for more papers by this authorJ. Michael Mathis
Gene Therapy Program, Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, Shreveport, LA, USA
Search for more papers by this authorMariam A. Stoff-Khalili
Division of Human Gene Therapy, Departments of Medicine, Obstetrics and Gynecology, Pathology, Surgery, and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL, USA
Department of Gynecology and Obstetrics, University of Duesseldorf, Medical Center, Duesseldorf, Germany
Search for more papers by this authorDavid T. Curiel
Division of Human Gene Therapy, Departments of Medicine, Obstetrics and Gynecology, Pathology, Surgery, and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL, USA
Search for more papers by this authorAlexander Stoff
Division of Human Gene Therapy, Departments of Medicine, Obstetrics and Gynecology, Pathology, Surgery, and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL, USA
Department of Plastic and Reconstructive Surgery, Dreifaltigkeits-Hospital, Wesseling, Germany
Department of Plastic and Reconstructive Surgery, University of Alabama at Birmingham, Birmingham, AL, USA
Search for more papers by this authorAngel A. Rivera
Division of Human Gene Therapy, Departments of Medicine, Obstetrics and Gynecology, Pathology, Surgery, and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL, USA
Search for more papers by this authorN. Sanjib Banerjee
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL, USA
Search for more papers by this authorSteven T. Moore
Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL, USA
Search for more papers by this authorT. Michael Numnum
Division of Human Gene Therapy, Departments of Medicine, Obstetrics and Gynecology, Pathology, Surgery, and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL, USA
Search for more papers by this authorAntonio Espinosa-de-los-Monteros
Department of Plastic and Reconstructive Surgery, Dreifaltigkeits-Hospital, Wesseling, Germany
Search for more papers by this authorDirk F. Richter
Department of Plastic and Reconstructive Surgery, Dreifaltigkeits-Hospital, Wesseling, Germany
Search for more papers by this authorGene P. Siegal
Division of Human Gene Therapy, Departments of Medicine, Obstetrics and Gynecology, Pathology, Surgery, and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL, USA
Search for more papers by this authorLouise T. Chow
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL, USA
Search for more papers by this authorDale Feldman
Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL, USA
Search for more papers by this authorLuis O. Vasconez
Department of Plastic and Reconstructive Surgery, University of Alabama at Birmingham, Birmingham, AL, USA
Search for more papers by this authorJ. Michael Mathis
Gene Therapy Program, Department of Cellular Biology and Anatomy, Louisiana State University Health Sciences Center, Shreveport, LA, USA
Search for more papers by this authorMariam A. Stoff-Khalili
Division of Human Gene Therapy, Departments of Medicine, Obstetrics and Gynecology, Pathology, Surgery, and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL, USA
Department of Gynecology and Obstetrics, University of Duesseldorf, Medical Center, Duesseldorf, Germany
Search for more papers by this authorDavid T. Curiel
Division of Human Gene Therapy, Departments of Medicine, Obstetrics and Gynecology, Pathology, Surgery, and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL, USA
Search for more papers by this authorAbstract
Abstract: The purpose of this study was to determine the effect of transplanted human mesenchymal stem cells (hMSCs) on wound healing. In this model, full-thickness cutaneous wounds were created by incision in the skin of adult New Zealand white rabbits and treated by transplanted hMSCs into the wounds. Wound healing was evaluated by histological analysis and tensiometry over time. A total of 15 New Zealand white rabbits with 10 wounds per animal were examined in this study. Animals were treated with hMSCs and euthanised at 3, 7, 14, 21 and 80 days after manipulation. The hMSCs were labelled with a fluorescent dye (CM-DiI), suspended in phosphate-buffered saline and used to treat full-thickness incisional wounds in rabbit skin. Tensiometry and histology were used to characterise the wound-healing rate of the incisional wounds. These results showed that transplanted hMSCs significantly inhibited scar formation and increased the tensile strength of the wounds. Importantly, MSCs from genetically unrelated donors did not appear to induce an immunologic response. In conclusion, human mesenchymal stem cell therapy is a viable approach to significantly affect the course of normal cutaneous wound healing and significantly increase the tensile strength.
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