‘HPV vulvitis’ revisited: frequent and persistent detection of novel epidermodysplasia verruciformis-associated HPV genotypes
Ming-Tseh Lin,
Angela Rohwedder,
Judith Mysliborski,
Kathryn Leopold, Vincent L. Wilson,
J. Andrew Carlson,
Ming-Tseh Lin
Division of Dermatology and Dermatopathology, Department of Pathology, Albany Medical College, Albany, NY, USA
Search for more papers by this authorAngela Rohwedder
Department of Microbiology and Virology, Ruhr University Bochum, Lalkar, Germany
Search for more papers by this authorJudith Mysliborski
Capital District Dermatology, Albany, NY, USA
Search for more papers by this authorVincent L. Wilson
Department of Environmental Studies, Louisiana State University, Baton Rouge, LA, USA
Search for more papers by this authorCorresponding Author
J. Andrew Carlson
Division of Dermatology and Dermatopathology, Department of Pathology, Albany Medical College, Albany, NY, USA
J. Andrew Carlson, MD, FRCP (C), Albany Medical College MC-81, 47 New Scotland Ave., Albany, NY 12208, USATel: +1 518 262 6252Fax: +1 518 262 6251e-mail: [email protected]Search for more papers by this authorMing-Tseh Lin,
Angela Rohwedder,
Judith Mysliborski,
Kathryn Leopold, Vincent L. Wilson,
J. Andrew Carlson,
Ming-Tseh Lin
Division of Dermatology and Dermatopathology, Department of Pathology, Albany Medical College, Albany, NY, USA
Search for more papers by this authorAngela Rohwedder
Department of Microbiology and Virology, Ruhr University Bochum, Lalkar, Germany
Search for more papers by this authorJudith Mysliborski
Capital District Dermatology, Albany, NY, USA
Search for more papers by this authorVincent L. Wilson
Department of Environmental Studies, Louisiana State University, Baton Rouge, LA, USA
Search for more papers by this authorCorresponding Author
J. Andrew Carlson
Division of Dermatology and Dermatopathology, Department of Pathology, Albany Medical College, Albany, NY, USA
J. Andrew Carlson, MD, FRCP (C), Albany Medical College MC-81, 47 New Scotland Ave., Albany, NY 12208, USATel: +1 518 262 6252Fax: +1 518 262 6251e-mail: [email protected]Search for more papers by this authorThis work was presented in part at the 21st Colloquim of the International Society of Dermatpathology, Graz, Austria, September 2000 and the United States and Canadian Academy of Pathology annual meeting, Atlanta, Georgia, Febuary 2006.
Abstract
Background: ‘Human papillomavirus (HPV) vulvitis’ is a disputed entity where most studies examining for genital-mucosal (GM) HPV have been negative.
Methods: Using degenerate and type specific primers for cutaneous (CU), GM and epidermodysplasia verruciformis (EV) HPV types, the prevalence of specific HPV types was investigated in biopsy specimens from 19 women with ‘HPV vulvitis’, seven with asymptomatic vulvar squamous papillomatosis (ASxVSP), and controls of vulvar fibroepithelial polyps (FEP) (15), vulvar condyloma (10) and normal vulva (NV) (10).
Results: HPV DNA/EV HPV/GM HPV/CU HPV were detected in 84/74/47/5% of vulvitis patients, 78/71/0/28% of ASxVSP, 47/20/20/7% of FEP, 10/10%/0/0 of NV and 100/0/100/10% of condyloma. Fourteen putatively novel HPV genotypes were detected in vulvitis and ASxVSP patients, but not in controls. The two most frequent novel EV HPV, Alb-4 and DL285, were detected in 9/19 (47%) and 5/19 (26%) of vulvitis cases and were persistently identified in serial biopsies. HPV co-infection and Alb-4 infection occurred significantly more frequently in vulvitis patients, particularly those complaining of ‘burning’ (62/62% vs. 17/7%, p ≤ 0.004). Koilocytosis was identified significantly more frequently in vulvitis compared with non-condyloma controls (81% vs. 40%, p = 0.0001), and its presence correlated with detection of HPV DNA (r = 0.3, p = 0.002).
Conclusion: The high frequency of novel EV HPV in HPV vulvitis and correlation of clinicopathologic findings with HPV DNA suggests that HPV vulvitis may indeed exist.
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