The inhibitory effect of UVB irradiation on the expression of p53 and Ki-67 proteins in arsenic-induced Bowen's disease
Chee-Yin Chai
Department of Pathology, Veterans General Hospital-Taipei, National Yang-Ming University, School of Medicine, Taipei, Taiwan, Republic of China
Search for more papers by this authorCorresponding Author
Hsin-Su Yu
Department of Dermatology, Veterans General Hospital-Taipei, National Yang-Ming University, School of Medicine, Taipei, Taiwan, Republic of China
Hsin-Su Yu, Department of Dermatology, Kaohsiung Medical College, No. 100, Shih-Chuan 1st Road, Kaohsiung City 807, Taiwan, Republic of China.Search for more papers by this authorHuai-Tzu Yen
Graduates Institute of Medical Sciences, Kaohsiung Medical College, Kaohsiung, Taiwan
Search for more papers by this authorKun-Bow Tsai
Department of Pathology, Veterans General Hospital-Taipei, National Yang-Ming University, School of Medicine, Taipei, Taiwan, Republic of China
Search for more papers by this authorGwu-Shing Chen
Department of Dermatology, Veterans General Hospital-Taipei, National Yang-Ming University, School of Medicine, Taipei, Taiwan, Republic of China
Search for more papers by this authorChia-Li Yu
Department of Medicine, Veterans General Hospital-Taipei, National Yang-Ming University, School of Medicine, Taipei, Taiwan, Republic of China
Search for more papers by this authorChee-Yin Chai
Department of Pathology, Veterans General Hospital-Taipei, National Yang-Ming University, School of Medicine, Taipei, Taiwan, Republic of China
Search for more papers by this authorCorresponding Author
Hsin-Su Yu
Department of Dermatology, Veterans General Hospital-Taipei, National Yang-Ming University, School of Medicine, Taipei, Taiwan, Republic of China
Hsin-Su Yu, Department of Dermatology, Kaohsiung Medical College, No. 100, Shih-Chuan 1st Road, Kaohsiung City 807, Taiwan, Republic of China.Search for more papers by this authorHuai-Tzu Yen
Graduates Institute of Medical Sciences, Kaohsiung Medical College, Kaohsiung, Taiwan
Search for more papers by this authorKun-Bow Tsai
Department of Pathology, Veterans General Hospital-Taipei, National Yang-Ming University, School of Medicine, Taipei, Taiwan, Republic of China
Search for more papers by this authorGwu-Shing Chen
Department of Dermatology, Veterans General Hospital-Taipei, National Yang-Ming University, School of Medicine, Taipei, Taiwan, Republic of China
Search for more papers by this authorChia-Li Yu
Department of Medicine, Veterans General Hospital-Taipei, National Yang-Ming University, School of Medicine, Taipei, Taiwan, Republic of China
Search for more papers by this authorAbstract
The aim of this study is to evaluate the effect of ultraviolet B (UVB) on arsenic-induced Bowen's disease. Four patients were irradiated with 750 mJ/cm2 of UVB and biopsies were performed before treatment and 2 weeks later. Immunohistochemical stains of p53 and Ki-67 were compared by the labelled-streptavidin method before and after the UVB treatment. We found that the number of p53 and Ki-67 positive cells after the UVB treatment were significantly fewer than those of non-UVB-treated specimens. These results suggest that the UVB inhibitory effect in Bowen's disease needs further studies to clarify its value in potentially retarding the progression of the hyperproliferative status in overt skin cancer on a molecular basis.
References
- 1 Tseng WP, Chu HM, How SW, Fong JM, Lin CS, Yeh S. Prevalence of skin cancer in an endemic area of chronic arsenicism in Taiwan. J Natl Cancer Inst 1968; 40: 453.
- 2
Yeh S,
How SW,
Lin CS.
Arsenical cancer of skin – histologic study with special reference to Bowen's disease.
Cancer
1968; 21: 312.
10.1002/1097-0142(196802)21:2<312::AID-CNCR2820210222>3.0.CO;2-K CAS PubMed Web of Science® Google Scholar
- 3 Lowe NJ, Prystowsky JH, Bourget T, Edelstein J, Nychay S, Armstrong R. Acitretin plus UVB therapy for psoriasis: comparisons with placebo plus UVB and acitretin alone. J Am Acad Dermatol 1991; 24: 591.
- 4 Ramsay DL, Lish KM, Yalowitz CB, Soter NA. Ultraviolet-B phototherapy for early stage cutaneous T-cell lymphoma. Arch Dermatol 1992; 128: 931.
- 5 Hollstein M, Sidransky D, Vogelstein B, Harris CC. p53 mutations in human cancers. Science 1991; 253: 49.
- 6 Porter PL, Gown AM, Kramp SG, Coltrera MD. Widespread p53 overexpression in human malignant tumors: an immunohistochemical study using methacarn-fixed, embedded tissue. Am J Pathol 1992; 140: 145.
- 7 Levine AR, Momand J, Finlay CA. The p53 tumour suppressor gene. Nature 1991; 351: 453.
- 8 Szekeres G, Giacomoni PD. Ki-67 and p53 expression in cutaneous Bowen's disease: an immunohistochemical study of fixed-embedded tissue section. Acta Derm Venereol 1994; 74: 95.
- 9 Campbell C, Quinn AG, Angus B, Rees JL. The relation between p53 mutation and p53 immunostaining in non-melanoma skin cancer. Br J Dermatol 1993; 129: 235.
- 10 Ro YS, Cooper PN, Lee JA, et al. p53 protein expression in benign and malignant skin tumours. Br J Dermatol 1993; 128: 237.
- 11 Sim CS, Slater SD, Mckee PH. Mutant p53 protein is expressed in Bowen's disease. Am J Dermatol 1992; 14: 195.
- 12 Deb S, Jackson CT, Subler MA, Martin DW. Modulation of cellular and viral promoters by mutant human p53 protein found in tumor cells. J Virol 1992; 66: 6164.
- 13 Linden MD, Nathanson SD, Zarbo R. Evaluation of anti-p53 antibody staining: quality control and technical considerations. Appl Immunohistochem 1994; 2: 218.
- 14 Humphrey PA, Franquemont DW, Geary WA, Kerns BM, Iglehart JD, Marks JR. Immunodetection of p53 protein in noninvasive epithelial proliferative breast disease. Appl Immunohistochem 1994; 2: 22.
- 15 Takata M, Matsui Y. Proliferating cell nuclear antigen (PC-NA) and p53 protein expression in Bowen's disease. J Dermatol 1994; 21: 947.
- 16 Brash DE, Rudolph JA, Simon JA, et al. A role of sunlight in skin cancer. UV-induced p53 mutations in squamous cell carcinoma. Proc Natl Acad Sci USA 1991; 88: 10124.
- 17 Rady P, Scinicariello F, Wagner RF Jr, Tyring SK. p53 mutations in basal cell carcinomas. Cancer Res 1992; 52: 3804.
- 18 Hall PA, McKee PH, Menage HP, Dover R, Lane DP. High levels of p53 protein in UV-irradiated normal skin. Oncogene 1993; 8: 203.
- 19 Liu M, Dhanwada KR, Bin DF, Hecht S, Pelling JC. Increase in p53 protein half-life in mouse keratinocytes following UV-B irradiation. Carcinogenesis 1994; 15: 1089.
- 20 Maltznian W, Czyzyk L. UV irradiation stimulates levels of p53 cellular tumor antigen in nontransformed cells. Mol Cell Biol 1984; 4: 1689.
- 21 Brown DC, Gatter KG. Monoclonal antibody Ki-67: its use in histopathology. Histopathology 1990; 17: 489.
- 22 Li JH, Rossman TG. Inhibition of DNA ligase activity by arsenite: a possible mechanism of its comutagenesis. Molec Toxicol 1989; 2: 1.
- 23 Jha AN, Noditi M, Nilson R, Natarajan RT. Genotoxic effects of sodium arsenite on human cells. Mutation Res 1992; 284: 215.
- 24 Studniberg HM, Weller P. PUVA, UVB, psoriasis, and non-melanoma skin cancer. J Am Acad Dermatol 1993; 29: 1013.
- 25 Epstein JH. Photocarcinogenesis, skin cancer, and aging. J Am Acad Dermatol 1983; 9: 487.
Citing Literature
