Volume 22, Issue 10 pp. 1193-1199

Enhancing bone formation by transplantation of a scaffold-free tissue-engineered periosteum in a rabbit model

Dongyang Ma,

Dongyang Ma

Department of Oral and Maxillofacial Surgery, Lanzhou General Hospital, Lanzhou Command of PLA, Gansu, China

Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Shaanxi, China

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Hong Yao,

Hong Yao

Department of Oral and Maxillofacial Surgery, Lanzhou General Hospital, Lanzhou Command of PLA, Gansu, China

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Wenyan Tian,

Wenyan Tian

Department of Oral and Maxillofacial Surgery, Lanzhou General Hospital, Lanzhou Command of PLA, Gansu, China

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Fulin Chen,

Fulin Chen

Rege Lab of Tissue Engineering, Department of Bioscience, Faculty of Life Science, Northwest University, Shaanxi, China

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Yanpu Liu,

Yanpu Liu

Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Shaanxi, China

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Tianqiu Mao,

Tianqiu Mao

Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Shaanxi, China

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Liling Ren,

Liling Ren

Department of Orthodontics, School of Stomatology, Lanzhou University, Gansu, China

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Dongyang Ma,

Dongyang Ma

Department of Oral and Maxillofacial Surgery, Lanzhou General Hospital, Lanzhou Command of PLA, Gansu, China

Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Shaanxi, China

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Hong Yao,

Hong Yao

Department of Oral and Maxillofacial Surgery, Lanzhou General Hospital, Lanzhou Command of PLA, Gansu, China

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Wenyan Tian,

Wenyan Tian

Department of Oral and Maxillofacial Surgery, Lanzhou General Hospital, Lanzhou Command of PLA, Gansu, China

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Fulin Chen,

Fulin Chen

Rege Lab of Tissue Engineering, Department of Bioscience, Faculty of Life Science, Northwest University, Shaanxi, China

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Yanpu Liu,

Yanpu Liu

Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Shaanxi, China

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Tianqiu Mao,

Tianqiu Mao

Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Shaanxi, China

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Liling Ren,

Liling Ren

Department of Orthodontics, School of Stomatology, Lanzhou University, Gansu, China

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First published: 08 February 2011

https://doi.org/10.1111/j.1600-0501.2010.02091.x

Citations: 36

Corresponding author:
Liling Ren
Department of Orthodontics
School of Stomatology
Lanzhou University
No. 199 Donggang West Road
Lanzhou
Gansu 730003
China
Tel.: +86 931 899 5013
Fax.: +86 931 899 5013
e-mail: [email protected]

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Abstract

Objectives: The periosteum plays an important role in bone regeneration. However, the harvesting of autogenous periosteum is associated with disadvantages such as donor site morbidity and limited donor sources. This study uses an osteogenic predifferentiated cell sheet to fabricate a scaffold-free tissue-engineered periosteum (TEP).

Material and methods: We generated an osteogenic predifferentiated cell sheet from rabbit bone marrow stromal cells (BMSCs) using a continuous culture system and harvested it using a scraping technique. Then, the in vitro characterization of the sheet was investigated using microscopy investigation, quantitative analysis of alkaline phosphatase (ALP) activity, and RT-PCR. Next, we demonstrated the in vivo osteogenic potential of the engineered sheet in ectopic sites together with a porous β-tricalcium phosphate ceramic. Finally, we evaluated its efficiency in treating delayed fracture healing after wrapping the cell sheet around the mandible in a rabbit model.

Results: The engineered periosteum showed sporadic mineralized nodules, elevated ALP activity, and up-regulated gene expression of osteogenic markers. After implantation in the subcutaneous pockets of the donor rabbits, the in vivo bone-forming capability of the engineered periosteum was confirmed by histological examinations. Additionally, when wrapping the engineered periosteum around a mandibular fracture gap, we observed improved bone healing and reduced amounts of fibrous tissue at the fracture site.

Conclusion: The osteogenic predifferentiated BMSC sheet can act as a scaffold-free TEP to facilitate bone regeneration. Hence, our study provides a promising strategy for enhancing bone regeneration in clinical settings.

To cite this article:
Ma D, Yao H, Tian W, Chen F, Liu Y, Mao T, Ren L. Enhancing bone formation by transplantation of a scaffold-free tissue-engineered periosteum in a rabbit model.
Clin. Oral Impl. Res. 22, 2011; 1193–1199.
doi: 10.1111/j.1600-0501.2010.02091.x

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Volume22, Issue10

October 2011

Pages 1193-1199

Enhancing bone formation by transplantation of a scaffold-free tissue-engineered periosteum in a rabbit model

Abstract

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Enhancing bone formation by transplantation of a scaffold-free tissue-engineered periosteum in a rabbit model

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