Correspondence to:
Anchun Mo
West China College of Stomatology
Sichuan University
Chengdu
China
Tel.: +86 28 85503563
Fax: +86 28 85503630
e-mail: [email protected]

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Abstract

Objective: The biosafety and efficacy of silver–hydroxyapatite–titania/polyamide nanocomposite (nAg–HA–TiO₂/PA) membrane as a guided bone regeneration (GBR) barrier were investigated based on a rat subcutaneous and critical-size calvarial defect model.

Material and methods: Thirty-six Sprague–Dawley albino rats were divided into nAg–HA–TiO₂/PA membrane test, expanded polytetrafluoroethylene (e-PTFE) membrane control and blank control. Inflammatory response and bone regeneration in each group were evaluated using morphological, serological, radiographic and histological techniques at 1, 4 and 8 weeks, respectively, after implantation.

Results: For subcutaneous implantation, slight degradation of nAg–HA–TiO₂/PA membranes was observed by scanning electron microscope at 4 and 8 weeks. Histopathologic examination demonstrated a thinner layer of granulation tissue in the vicinity of nAg–HA–TiO₂/PA membranes than that of e-PTFE membranes. For cranial defect implantation, the serum alkaline phosphatase level was remarkably higher in nAg–HA–TiO₂/PA group than that in e-PTFE group. Radiographic and histomorphometric analysis showed a fully closed cranial defect for both nAg–HA–TiO₂/PA and e-PTFE groups at 8 weeks. No remarkable difference was found between the two groups regarding the integral optical density of neo-bone at each time interval.

Conclusion: nAg–HA–TiO₂/PA membranes demonstrated better biocompatibility and similar osteoinductive activity compared with e-PTFE membranes. nAg–HA–TiO₂/PA composite membranes provided a good prospect for further research and development in anti-bacterial GBR membrane.

To cite this article:
Zhang JC, Xu Q, Huang C, Mo AC, Li JD, Zuo Y. Biological properties of an anti-bacterial membrane for guided bone regeneration: an experimental study in rats.
Clin. Oral Impl. Res. 21, 2010; 321–327.
doi: 10.1111/j.1600-0501.2009.01838.x

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Volume21, Issue3

March 2010

Pages 321-327

Biological properties of an anti-bacterial membrane for guided bone regeneration: an experimental study in rats

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Biological properties of an anti-bacterial membrane for guided bone regeneration: an experimental study in rats

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