Correspondence to:
Priv. Doz. Dr Frank Schwarz
Department of Oral Surgery
Westdeutsche Kieferklinik
Heinrich Heine University
D-40225 Duesseldorf
Germany
Tel.: +49 211 8118149
Fax: +49 211 1713542
e-mail: [email protected]

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Abstract

Aim: The aim of the present study was to investigate the pattern of biodegradation of different polyethylene glycol (PEG) hydrogel/RGD-peptide modifications in rats.

Material and methods: Two different hydrogels were employed: (i) a combination of four-arm PEG-thiol, M_n=2.3 kDa, and eight-arm PEG-acrylate, M_n=2.3 kDa (PEG1); and (ii) a combination of four-arm PEG-thiol, M_n=2.3 kDa, and four-arm PEG-acrylate, M_n=15 kDa (PEG2). Both PEG1 and PEG2 were either used alone or combined with a nine amino acid cys-RGD peptide (RGD). A non-cross-linked porcine type I and III collagen membrane [BioGide^® (BG)] served as control. Specimens were randomly allocated in unconnected subcutaneous pouches separated surgically on the back of 60 wistar rats, which were divided into six groups (1, 2, 4, 8, 16, and 24 weeks). Specimens were prepared for histological (tissue integration, foreign body reactions, biodegradation) and immunohistochemical (angiogenesis) analysis.

Results: All materials investigated revealed unimpeded and comparable tissue integration without any signs of foreign body reactions. While BG exhibited transmembraneous blood vessel formation at 1 week, all PEG specimens were just surrounded by a well-vascularized connective tissue. The hydrolytic disruption of PEG1 and PEG1/RGD specimens was associated with an ingrowth of blood vessels at 4 weeks. Biodegradation times were highest for PEG1 (24 weeks)>PEG1/RGD (16 weeks)>BG (4 weeks)>PEG2=PEG2/RGD (2 weeks).

Conclusion: Within the limits of the present study, it was concluded that (i) all materials investigated revealed a high biocompatibility and tissue integration, and (ii) hydrogel biodegradation was dependent on PEG composition.

References

Buemi, M., Galeano, M., Sturiale, A., Ientile, R., Crisafulli, C., Parisi, A., Catania, M., Calapai, G., Impala, P., Aloisi, C., Squadrito, F., Altavilla, D., Bitto, A., Tuccari, G. & Frisina, N. (2004) Recombinant human erythropoietin stimulates angiogenesis and healing of ischemic skin wounds. Shock 22: 169–173.
10.1097/01.shk.0000133591.47776.bd
CAS PubMed Web of Science® Google Scholar
Bunyaratavej, P. & Wang, H.L. (2001) Collagen membranes: a review. Journal of Periodontology 72: 215–229.
10.1902/jop.2001.72.2.215
CAS PubMed Web of Science® Google Scholar
Donath, K. (1985) The diagnostic value of the new method for the study of undecalcified bones and teeth with attached soft tissue (Säge–Schliff (sawing and grinding) technique). Pathology Research and Practice 179: 631–633.
10.1016/S0344-0338(85)80209-0
PubMed Web of Science® Google Scholar
Elbert, D.L., Pratt, A.B., Lutolf, M.P., Halstenberg, S. & Hubbell, J.A. (2001) Protein delivery from materials formed by self-selective conjugate addition reactions. Journal of Controlled Release 76: 11–25.
10.1016/S0168-3659(01)00398-4
CAS PubMed Web of Science® Google Scholar
Greenstein, G. & Caton, J.G. (1993) Biodegradable barriers and guided tissue regeneration. Periodontology 2000 1: 36–45.
10.1111/j.1600-0757.1993.tb00205.x
PubMed Google Scholar
Hämmerle, C.H. & Jung, R.E. (2003) Bone augmentation by means of barrier membranes. Periodontology 2000 33: 36–53.
10.1046/j.0906-6713.2003.03304.x
PubMed Web of Science® Google Scholar
Hardwick, R., Scantlebury, T.V., Sanchez, R., Whitley, N. & Ambruster, J. (1994) Membrane design criteria for guided bone regeneration of the alveolar ridge. In: D. Buser, C. Dahlin & R.K. Schenk, eds. Guided Bone Regeneration in Implant Dentistry. 1st edition, 101–137. Chicago: Quintessence Publishing Co.
Google Scholar
Haroon, Z.A., Hettasch, J.M., Lai, T.S., Dewhirst, M.W. & Greenberg, C.S. (1999) Tissue transglutaminase is expressed, active, and directly involved in rat dermal wound healing and angiogenesis. FASEB Journal 13: 1787–1795.
10.1096/fasebj.13.13.1787
CAS PubMed Web of Science® Google Scholar
Jung, R.E., Cochran, D.L., Domken, O., Seibl, R., Jones, A.A., Buser, D. & Hämmerle, C.H. (2007b) The effect of matrix bound parathyroid hormone on bone regeneration. Clinical Oral Implants Research 18: 319–325.
10.1111/j.1600-0501.2007.01342.x
PubMed Web of Science® Google Scholar
Jung, R.E., Hämmerle, C.H., Kokovic, V. & Weber, F.E. (2007a) Bone regeneration using a synthetic matrix containing a parathyroid hormone peptide combined with a grafting material. International Journal of Oral & Maxillofacial Implants 22: 258–266.
PubMed Web of Science® Google Scholar
Jung, R.E., Zwahlen, R., Weber, F.E., Molenberg, A., Van Lenthe, G.H. & Hammerle, C.H. (2006) Evaluation of an in situ formed synthetic hydrogel as a biodegradable membrane for guided bone regeneration. Clinical Oral Implants Research 17: 426–433.
10.1111/j.1600-0501.2005.01228.x
PubMed Web of Science® Google Scholar
Long, M.W., Robinson, J.A., Ashcraft, E.A. & Mann, K.G. (1995) Regulation of human bone marrow-derived osteoprogenitor cells by osteogenic growth factors. Journal of Clinical Investigation 95: 881–887.
10.1172/JCI117738
CAS PubMed Web of Science® Google Scholar
Muller, U., Bossy, B., Venstrom, K. & Reichardt, L.F. (1995) Integrin alpha 8 beta 1 promotes attachment, cell spreading, and neurite outgrowth on fibronectin. Molecular Biology of the Cell 6: 433–448.
10.1091/mbc.6.4.433
CAS PubMed Web of Science® Google Scholar
Postlethwaite, A.E., Seyer, J.M. & Kang, A.H. (1978) Chemotactic attraction of human fibroblasts to type I, II, and III collagens and collagen-derived peptides. Proceedings of the National Academy of Sciences of the United States of America 75: 871–875.
10.1073/pnas.75.2.871
CAS PubMed Web of Science® Google Scholar
Reilly, T.M., Seldes, R., Luchetti, W. & Brighton, C.T. (1998) Similarities in the phenotypic expression of pericytes and bone cells. Clinical Orthopaedics and Related Research Jan (346): 95–103.
Google Scholar
Rickard, D.J., Kassem, M., Hefferan, T.E., Sarkar, G., Spelsberg, T.C. & Riggs, B.L. (1996) Isolation and characterization of osteoblast precursor cells from human bone marrow. Journal of Bone and Mineral Research 11: 312–324.
10.1002/jbmr.5650110305
CAS PubMed Web of Science® Google Scholar
Rothamel, D., Schwarz, F., Sager, M., Herten, M., Sculean, A. & Becker, J. (2005) Biodegradation of differently cross-linked collagen membranes: an experimental study in the rat. Clinical Oral Implants Research 16: 369–378.
10.1111/j.1600-0501.2005.01108.x
CAS PubMed Web of Science® Google Scholar
Rothamel, D., Schwarz, F., Sculean, A., Herten, M., Scherbaum, W. & Becker, J. (2004) Biocompatibility of various collagen membranes in cultures of human PDL fibroblasts and human osteoblast-like cells. Clinical Oral Implants Research 15: 443–449.
10.1111/j.1600-0501.2004.01039.x
CAS PubMed Web of Science® Google Scholar
Ruoslahti, E. (1996) RGD and other recognition sequences for integrins. Annual Review of Cell and Developmental Biology 12: 697–715.
10.1146/annurev.cellbio.12.1.697
CAS PubMed Web of Science® Google Scholar
Schwarz, F., Herten, M., Sager, M., Wieland, M., Dard, M. & Becker, J. (2007) Histological and immunohistochemical analysis of initial and early osseous integration at chemically modified and conventional SLA titanium implants: preliminary results of a pilot study in dogs. Clinical Oral Implants Research 18: 481–488.
10.1111/j.1600-0501.2007.01341.x
PubMed Web of Science® Google Scholar
Schwarz, F., Rothamel, D., Herten, M., Sager, D., Ferrari, D. & Becker, J. (2008b) Immunohistochemical characterization of guided bone regeneration at dehiscence-type defect using different barrier membranes. An experimental study in dogs. Clinical Oral Implants Research 19: 402–415.
10.1111/j.1600-0501.2007.01486.x
CAS PubMed Web of Science® Google Scholar
Schwarz, F., Rothamel, D., Herten, M., Sager, M. & Becker, J. (2006) Angiogenesis pattern of native and cross-linked collagen membranes: an immunohistochemical study in the rat. Clinical Oral Implants Research 17: 403–409.
10.1111/j.1600-0501.2005.01225.x
CAS PubMed Web of Science® Google Scholar
Schwarz, F., Sager, M., Ferrari, D., Herten, M., Wieland, M. & Becker, J. (2008a) Bone regeneration in dehiscence-type defects at non-submerged and submerged chemically modified (SLActive) and conventional SLA titanium implants: an immunohistochemical study in dogs. Journal of Clinical Periodontology 35: 64–75.
10.1111/j.1600-051X.2007.01159.x
PubMed Web of Science® Google Scholar
Sela, M.N., Kohavi, D., Krausz, E., Steinberg, D. & Rosen, G. (2003) Enzymatic degradation of collagen-guided tissue regeneration membranes by periodontal bacteria. Clinical Oral Implants Research 14: 263–268.
10.1034/j.1600-0501.2003.140302.x
PubMed Web of Science® Google Scholar
Selvig, K.A., Kersten, B.G., Chamberlain, A.D., Wikesjo, U.M. & Nilveus, R.E. (1992) Regenerative surgery of intrabony periodontal defects using ePTFE barrier membranes: scanning electron microscopic evaluation of retrieved membranes versus clinical healing. Journal of Periodontology 63: 974–978.
10.1902/jop.1992.63.12.974
PubMed Web of Science® Google Scholar
Speer, D.P., Chvapil, M., Eskelson, C.D. & Ulreich, J. (1980) Biological effects of residual glutaraldehyde in glutaraldehyde-tanned collagen biomaterials. Journal of Biomedical Materials Research 14: 753–764.
10.1002/jbm.820140607
CAS PubMed Web of Science® Google Scholar
Tempro, P.J. & Nalbandian, J. (1993) Colonization of retrieved polytetrafluoroethylene membranes: morphological and microbiological observations. Journal of Periodontology 64: 162–168.
10.1902/jop.1993.64.3.162
PubMed Web of Science® Google Scholar
Von Arx, T., Broggini, N., Jensen, S.S., Bornstein, M.M., Schenk, R.K. & Buser, D. (2005) Membrane durability and tissue response of different bioresorbable barrier membranes: a histologic study in the rabbit calvarium. International Journal of Oral & Maxillofacial Implants 20: 843–853.
PubMed Web of Science® Google Scholar
Wechsler, S., Fehr, D., Molenberg, A., Raeber, G., Schense, J.C. & Weber, F.E. (2008) A novel, tissue occlusive poly(ethylene glycol) hydrogel material. Journal of Biomedical Materials Research A 85: 285–292.
10.1002/jbm.a.31477
CAS PubMed Web of Science® Google Scholar
Working, P.K., Newman, M.S., Jonhson, J. & Cornacoff, J.B. (1997) Safety of poly(ethylene glycol) and poly(ethylene glycol) derivates. In: S.J. Zalipsky & J.M. Harris, eds. Poly(Ethylene Glycol), Chemistry and Biological Applications, 45–57. Washington, DC: American Chemical Society.
10.1021/bk-1997-0680.ch004
Web of Science® Google Scholar

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Volume20, Issue2

February 2009

Pages 116-125

Biodegradation of different synthetic hydrogels made of polyethylene glycol hydrogel/RGD-peptide modifications: an immunohistochemical study in rats

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Biodegradation of different synthetic hydrogels made of polyethylene glycol hydrogel/RGD-peptide modifications: an immunohistochemical study in rats

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