Altered distribution of tight junction proteins after intestinal ischaemia/reperfusion injury in rats
Qiurong Li
Institute of General Surgery, Jinling Hospital, Nanjing, China
School of Medicine, Nanjing University, Nanjing, China
Search for more papers by this authorQiang Zhang
Institute of General Surgery, Jinling Hospital, Nanjing, China
School of Medicine, Nanjing University, Nanjing, China
Search for more papers by this authorChenyang Wang
Institute of General Surgery, Jinling Hospital, Nanjing, China
Search for more papers by this authorXiaoxiang Liu
National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China
Search for more papers by this authorLinlin Qu
Institute of General Surgery, Jinling Hospital, Nanjing, China
School of Medicine, Nanjing University, Nanjing, China
Search for more papers by this authorLili Gu
Institute of General Surgery, Jinling Hospital, Nanjing, China
Search for more papers by this authorNing Li
Institute of General Surgery, Jinling Hospital, Nanjing, China
Search for more papers by this authorCorresponding Author
Jieshou Li
Institute of General Surgery, Jinling Hospital, Nanjing, China
Correspondence to: Jieshou LI, Institute of General Surgery, Jinling Hospital, No. 305 East Zhongshan Road, Nanjing 210002, China.Tel.: 86-25-80860064Fax: 86-25-84803956E-mail: [email protected]Search for more papers by this authorQiurong Li
Institute of General Surgery, Jinling Hospital, Nanjing, China
School of Medicine, Nanjing University, Nanjing, China
Search for more papers by this authorQiang Zhang
Institute of General Surgery, Jinling Hospital, Nanjing, China
School of Medicine, Nanjing University, Nanjing, China
Search for more papers by this authorChenyang Wang
Institute of General Surgery, Jinling Hospital, Nanjing, China
Search for more papers by this authorXiaoxiang Liu
National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China
Search for more papers by this authorLinlin Qu
Institute of General Surgery, Jinling Hospital, Nanjing, China
School of Medicine, Nanjing University, Nanjing, China
Search for more papers by this authorLili Gu
Institute of General Surgery, Jinling Hospital, Nanjing, China
Search for more papers by this authorNing Li
Institute of General Surgery, Jinling Hospital, Nanjing, China
Search for more papers by this authorCorresponding Author
Jieshou Li
Institute of General Surgery, Jinling Hospital, Nanjing, China
Correspondence to: Jieshou LI, Institute of General Surgery, Jinling Hospital, No. 305 East Zhongshan Road, Nanjing 210002, China.Tel.: 86-25-80860064Fax: 86-25-84803956E-mail: [email protected]Search for more papers by this authorThe authors declare no conflict of interest.
Abstract
Tight junction (TJ) disruptions have been demonstrated both in vitro and more recently in vivo in infection. However, the molecular basis for changes of TJ during ischaemia-reperfusion (I/R) injury is poorly understood. In the present study, intestinal damage was induced by I/R in an animal model. As assessed by TUNEL and propidium iodide uptake, we showed that I/R injury induced apoptosis as well as necrosis in rat colon, and the frequency of apoptotic and necrotic cells reached the maximum at 5 hrs of reperfusion. Immunofluorescence microscopy revealed that claudins 1, 3 and 5 are strongly expressed in the surface epithelial cells of the colon; however, labelling of all three proteins was present diffusely within cells and no longer focused at the lateral cell boundaries after I/R. Using Western blot analysis, we found that distribution of TJ proteins in membrane microdomains of TJ was markedly affected in I/R injury rats. Occludin, ZO-1, claudin-1 and claudin-3 were completely displaced from TX-100 insoluble fractions to TX-100 soluble fractions, and claudin-5 was partly displaced. The distribution of lipid raft marker protein caveolin-1 was also changed after I/R. I/R injury results in the disruption of TJs, which characterized by relocalization of the claudins 1, 3 and 5 and an increase in intestinal permeability using molecular tracer measurement. I/R injury altered distribution of TJ proteins in vivo that was associated with functional TJ deficiencies.
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