A method for the identification of proteins secreted by lactic acid bacteria grown in complex media

Borja Sánchez

Laboratoire de Microbiologie et Biochimie Appliquée, Université de Bordeaux, UMR 5248 CBMN, UBX1-ENITAB, ENITAB, Gradignan, France

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Sthéphane Chaignepain,

Sthéphane Chaignepain

Université de Bordeaux, UMR 5248 CBMN, UBX1-ENITAB, Institut Européen de Chimie et Biologie 2, Pessac, France

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Jean-Marie Schmitter,

Jean-Marie Schmitter

Université de Bordeaux, UMR 5248 CBMN, UBX1-ENITAB, Institut Européen de Chimie et Biologie 2, Pessac, France

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María C. Urdaci,

María C. Urdaci

Laboratoire de Microbiologie et Biochimie Appliquée, Université de Bordeaux, UMR 5248 CBMN, UBX1-ENITAB, ENITAB, Gradignan, France

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Borja Sánchez,

Borja Sánchez

Laboratoire de Microbiologie et Biochimie Appliquée, Université de Bordeaux, UMR 5248 CBMN, UBX1-ENITAB, ENITAB, Gradignan, France

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Sthéphane Chaignepain,

Sthéphane Chaignepain

Université de Bordeaux, UMR 5248 CBMN, UBX1-ENITAB, Institut Européen de Chimie et Biologie 2, Pessac, France

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Jean-Marie Schmitter,

Jean-Marie Schmitter

Université de Bordeaux, UMR 5248 CBMN, UBX1-ENITAB, Institut Européen de Chimie et Biologie 2, Pessac, France

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María C. Urdaci,

María C. Urdaci

Laboratoire de Microbiologie et Biochimie Appliquée, Université de Bordeaux, UMR 5248 CBMN, UBX1-ENITAB, ENITAB, Gradignan, France

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First published: 15 May 2009

https://doi.org/10.1111/j.1574-6968.2009.01599.x

Citations: 5

Correspondence: Borja Sánchez, Laboratoire de Microbiologie et Biochimie Appliquée, Université de Bordeaux, UMR 5248 CBMN, UBX1-ENITAB, ENITAB, 1 cours du Général de Gaulle, 33175 Gradignan Cedex, France. Tel.: +33 5 57 35 59 92; fax: +33 5 57 35 07 39; e-mail: [email protected]

Editor: Wolfgang Kneifel

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Abstract

Lactic acid bacteria (LAB) are known for their special nutritional requirements, being usually cultured in complex media to achieve optimal growth. In this paper, a protocol based on trichloroacetic acid precipitation of peptides and proteins is presented. The method has been tested on four probiotic LAB strains grown in De Man Rogosa Sharpe (MRS) broth, a complex medium that is often used for the culture of such bacteria. This protocol allowed the detection of 19 proteins after sodium dodecyl sulfate-polyacrylamide gel electrophoresis, 10 of them being successfully identified by tandem MS. Thereafter, the 10 were found to be secreted or surface associated by bioinformatic means. In conclusion, this work supplies a method for the identification of proteins secreted by LAB, allowing discrimination between the proteins present in the MRS and those produced by probiotic LAB.

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Volume295, Issue2

June 2009

Pages 226-229

A method for the identification of proteins secreted by lactic acid bacteria grown in complex media

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A method for the identification of proteins secreted by lactic acid bacteria grown in complex media

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