Listeria monocytogenes infection of Caco-2 human epithelial cells induces activation of transcription factor NF-κB/Rel-like DNA binding activities
Nadja Hauf
Lehrstuhl für Mikrobiologie, Theodor-Boveri-Institut für Biowissenschaften der Universität Würzburg, Am Hubland, 97074 Würzburg, Germany
1Institut für Medizinische Mikrobiologie der Universität Giessen, Frankfurter Straße 107, 35392 Giessen, Germany.
Search for more papers by this authorWerner Goebel
Lehrstuhl für Mikrobiologie, Theodor-Boveri-Institut für Biowissenschaften der Universität Würzburg, Am Hubland, 97074 Würzburg, Germany
Search for more papers by this authorFranz Fiedler
Lehrstuhl für Mikrobiologie, Universität München, 80638 München, Germany
Search for more papers by this authorCorresponding Author
Michael Kuhn
Lehrstuhl für Mikrobiologie, Theodor-Boveri-Institut für Biowissenschaften der Universität Würzburg, Am Hubland, 97074 Würzburg, Germany
*Corresponding author. Tel.: +49 (931) 8884421; Fax: +49 (931) 8884402, E-mail address: [email protected]Search for more papers by this authorNadja Hauf
Lehrstuhl für Mikrobiologie, Theodor-Boveri-Institut für Biowissenschaften der Universität Würzburg, Am Hubland, 97074 Würzburg, Germany
1Institut für Medizinische Mikrobiologie der Universität Giessen, Frankfurter Straße 107, 35392 Giessen, Germany.
Search for more papers by this authorWerner Goebel
Lehrstuhl für Mikrobiologie, Theodor-Boveri-Institut für Biowissenschaften der Universität Würzburg, Am Hubland, 97074 Würzburg, Germany
Search for more papers by this authorFranz Fiedler
Lehrstuhl für Mikrobiologie, Universität München, 80638 München, Germany
Search for more papers by this authorCorresponding Author
Michael Kuhn
Lehrstuhl für Mikrobiologie, Theodor-Boveri-Institut für Biowissenschaften der Universität Würzburg, Am Hubland, 97074 Würzburg, Germany
*Corresponding author. Tel.: +49 (931) 8884421; Fax: +49 (931) 8884402, E-mail address: [email protected]Search for more papers by this authorAbstract
The effect of Listeria monocytogenes infection on the cellular level of transcription factor NF-κB in the human epithelia-like cell line Caco-2 was investigated. Infection with L. monocytogenes or treatment with lipoteichoic acid induced the formation of three NF-κB-like DNA-protein complexes C1, C2, and C3, which were identified as containing either, RelA and p50, RelB and p50, or p50, respectively. NF-κB activation in L. monocytogenes-infected Caco-2 cells was distinct from NF-κB activation in infected P388D1 macrophages concerning the NF-κB complexes formed and the kinetics of the induction.
References
- [1] Schuchat, A., Swaminathan, B., Broome, C.V. (1991) Epidemiology of human listeriosis. Clin. Microbiol. Rev. 4, 169–183.
- [2] Kuhn, M., Goebel, W. (1995) Molecular studies on the virulence of Listeria monocytogenes. Genet. Eng. 17, 31–51.
- [3] Sheehan, B., Kocks, C., Dramsi, S., Gouin, E., Klarsfeld, A.D., Mengaud, J., Cossart, P. (1994) Molecular and genetic determinants of the Listeria monocytogenes infectious process. Curr. Top. Microbiol. Immunol. 192, 187–216.
- [4] Schwan, W.R., Goebel, W. (1994) Host cell responses to Listeria monocytogenes infection include differential transcription of host stress genes involved in signal transduction. Proc. Natl. Acad. Sci. USA 91, 6428–6432.
- [5] Demuth, A., Goebel, W., Beuscher, H.U., Kuhn, M. (1996) Differential regulation of cytokine- and cytokine receptor mRNA expression upon infection of bone marrow-derived macrophages with Listeria monocytogenes. Infect. Immun. 64, 3475–3483.
- [6] Arnold, R., Scheffer, J., König, B., König, W. (1993) Effects of Listeria monocytogenes and Yersinia enterocolitica on cytokine gene expression and release from human polymorphonuclear granulocytes and epithelial (HEp-2) cells. Infect. Immun. 61, 2545–2555.
- [7] Eckmann, L., Kagnoff, M.F., Fierer, J. (1993) Epithelial cells secrete the chemokine interleukin-8 in response to bacterial entry. Infect. Immun. 61, 4569–4574.
- [8] Siebenlist, U., Franzoso, G., Brown, J. (1994) Structure, regulation and function of NF-κB. Annu. Rev. Cell Biol. 10, 405–455.
- [9] Nolan, G.P., Ghosh, S., Liou, H.L., Tempst, P., Baltimore, D. (1991) DNA binding and IκB inhibition of the cloned p65 subunit of NF-κB, a rel-related polypeptide. Cell 64, 961–969.
- [10] Lernbecher, T., Kistler, B., Wirth, T. (1994) Two distinct mechanisms contribute to the constitutive activation of RelB in lymphoid cells. EMBO J. 13, 4060–4069.
- [11] Busam, K., Gieringer, C., Freudenberg, M., Hohmann, H.P. (1992) Staphylococcus aureus and derived exotoxins induce nuclear factor κB-like activity in murine bone marrow macrophages. Infect. Immun. 60, 2008–2015.
- [12] Dyer, R.B., Collaco, C.R., Niesel, D.W., Herzog, N.K. (1993) Shigella flexneri invasion of HeLa cells induce NF-κB DNA-binding activity. Infect. Immun. 61, 4427–4433.
- [13] Sporn, A.L., Sahni, S.K., Lerner, N.B., Marder, V.J., Silverman, D.J., Turpin, L.C., Schwab, A.L. (1997) Rickettsia rickettsii infection of cultured human endothelial cells induces NF-κB activation. Infect. Immun. 65, 2786–2791.
- [14] Eaves-Pyles, T., Szabo, C., Salzman, A.L. (1999) Bacterial invasion is not required for activation of NF-κB in enterocytes. Infect. Immun. 67, 800–804.
- [15] Spellerberg, B., Rosenow, C., Sha, W., Toumanen, E.I. (1996) Pneumococcal cell wall activates NF-κB in human monocytes: aspects distinct from endotoxin. Microb. Pathog. 20, 309–317.
- [16] Hauf, N., Goebel, W., Serfling, E., Kuhn, M. (1994) Listeria monocytogenes infection enhances transcription factor NF-κB in P388D1 macrophage-like cells. Infect. Immun. 62, 2740–2747.
- [17] Hauf, N., Goebel, W., Fiedler, F., Sokolovic, Z., Kuhn, M. (1997) Listeria monocytogenes infection of P388D1 macrophages results in a biphasic NF-κB (RelA/p50) activation induced by lipoteichoic acid and bacterial phospholipases and mediated by IκBα and IκBβ degradation. Proc. Natl. Acad. Sci. USA 94, 9394–9399.
- [18] Leimeister-Wächter, M., Chakraborty, T. (1989) Detection of listeriolysin, the thiol-dependent hemolysin in Listeria monocytogenes, Listeria ivanovii, and Listeria seeligeri. Infect. Immun. 57, 2350–2357.
- [19] Schreck, R., Rieber, P., Baeuerle, P.A. (1991) Reactive oxygen intermediates as apparently widely used messengers in the activation of the NF-κB transcription factor and HIV-1. EMBO J. 10, 2247–2258.
- [20]
Fischer, W.,
Koch, H.U.,
Haas, R. (1983) Improved preparation of lipoteichoic acid.
Eur. J. Biochem.
133, 523–530.
10.1111/j.1432-1033.1983.tb07495.x Google Scholar
- [21] Ruhland, G.J., Fiedler, F. (1987) Occurrence and biochemistry of lipoteichoic acids in the genus Listeria. Syst. Appl. Microbiol. 9, 40–46.
- [22] Pron, B., Boumaila, C., Jaubert, F., Sarnacki, S., Monnet, J.P., Berche, P., Gaillard, J.L. (1998) Comprehensive study of the intestinal stage of listeriosis in a rat ligated ileal loop system. Infect. Immun. 66, 747–755.
- [23] Krieger, M., Acton, S., Ashkenas, J., Pearson, A., Penman, M., Resnick, D. (1993) Molecular flypaper, host defense, and atherosclerosis. Structure, binding properties, and functions of macrophage scavenger receptors. J. Biol. Chem. 268, 4569–4572.
- [24] Wright, S.D. (1995) CD14 and innate recognition of bacteria. J. Immunol. 155, 6–8.
