The broad host range plasmid pLF1311 from Lactobacillus fermentum VKM1311
Vladimir V. Aleshin
State Research Institute of Genetics and Selection of Industrial Microorganisms (GNIIGenetika), 1 Dorozhny proezd, 1, Moscow 113545, Russia
Research Institute of Physiology, Biochemistry and Nutrition of Livestock, Russian Academy of Agricultural Sciences, Kaluga region, Borovsk 249010, Russia
Search for more papers by this authorElena V. Semenova
State Research Institute of Genetics and Selection of Industrial Microorganisms (GNIIGenetika), 1 Dorozhny proezd, 1, Moscow 113545, Russia
Research Institute of Physiology, Biochemistry and Nutrition of Livestock, Russian Academy of Agricultural Sciences, Kaluga region, Borovsk 249010, Russia
Search for more papers by this authorVera G. Doroshenko
State Research Institute of Genetics and Selection of Industrial Microorganisms (GNIIGenetika), 1 Dorozhny proezd, 1, Moscow 113545, Russia
Search for more papers by this authorYurgis V. Jomantas
State Research Institute of Genetics and Selection of Industrial Microorganisms (GNIIGenetika), 1 Dorozhny proezd, 1, Moscow 113545, Russia
Search for more papers by this authorBoris V. Tarakanov
Research Institute of Physiology, Biochemistry and Nutrition of Livestock, Russian Academy of Agricultural Sciences, Kaluga region, Borovsk 249010, Russia
Search for more papers by this authorCorresponding Author
Vitaliy A. Livshits
State Research Institute of Genetics and Selection of Industrial Microorganisms (GNIIGenetika), 1 Dorozhny proezd, 1, Moscow 113545, Russia
*Corresponding author. Tel.: +7 (095) 314-26-95; Fax: +7 (095) 315-05-01, E-mail address: [email protected]Search for more papers by this authorVladimir V. Aleshin
State Research Institute of Genetics and Selection of Industrial Microorganisms (GNIIGenetika), 1 Dorozhny proezd, 1, Moscow 113545, Russia
Research Institute of Physiology, Biochemistry and Nutrition of Livestock, Russian Academy of Agricultural Sciences, Kaluga region, Borovsk 249010, Russia
Search for more papers by this authorElena V. Semenova
State Research Institute of Genetics and Selection of Industrial Microorganisms (GNIIGenetika), 1 Dorozhny proezd, 1, Moscow 113545, Russia
Research Institute of Physiology, Biochemistry and Nutrition of Livestock, Russian Academy of Agricultural Sciences, Kaluga region, Borovsk 249010, Russia
Search for more papers by this authorVera G. Doroshenko
State Research Institute of Genetics and Selection of Industrial Microorganisms (GNIIGenetika), 1 Dorozhny proezd, 1, Moscow 113545, Russia
Search for more papers by this authorYurgis V. Jomantas
State Research Institute of Genetics and Selection of Industrial Microorganisms (GNIIGenetika), 1 Dorozhny proezd, 1, Moscow 113545, Russia
Search for more papers by this authorBoris V. Tarakanov
Research Institute of Physiology, Biochemistry and Nutrition of Livestock, Russian Academy of Agricultural Sciences, Kaluga region, Borovsk 249010, Russia
Search for more papers by this authorCorresponding Author
Vitaliy A. Livshits
State Research Institute of Genetics and Selection of Industrial Microorganisms (GNIIGenetika), 1 Dorozhny proezd, 1, Moscow 113545, Russia
*Corresponding author. Tel.: +7 (095) 314-26-95; Fax: +7 (095) 315-05-01, E-mail address: [email protected]Search for more papers by this authorAbstract
The complete nucleotide sequence (2389 bp) of the cryptic plasmid pLF1311 from Lactobacillus fermentum VKM1311 was determined. DNA sequence analysis revealed the putative coding regions for a replicative protein (RepB), its repressor (RepA) and double-stranded (dso) and single-stranded (sso) origins. pLF1311 belongs to the pE194 family of rolling circle-replicating plasmids. A derivative of pLF1311 that contains the cat gene of plasmid pC194 of Staphylococcus aureus and the oriT of RP4 was constructed and transferred by conjugative mobilization from Escherichia coli to various Gram-positive bacteria. The stable maintenance of this derivative was shown in some strains of Lactobacillus, Lactococcus, Enterococcus and Bacillus under non-selective conditions.
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