Use of a non-adherent cell culture system for testing the effect of 2′,3′-dideoxyinosine against Cryptosporidium parvum
Corresponding Author
Philippe Lawton
Département Pharmaceutique de Parasitologie et Mycologie Médicale, Faculté de Pharmacie, Université Claude-Bernard, F-69373 Lyon Cedex 08, France
*Corresponding author. Tel.: +33 4 78 77 72 77; Fax: +33 4 78 77 71 58, E-mail address: [email protected]Search for more papers by this authorCarine Hejl
Département Pharmaceutique de Parasitologie et Mycologie Médicale, Faculté de Pharmacie, Université Claude-Bernard, F-69373 Lyon Cedex 08, France
Search for more papers by this authorMarie-Elisabeth Sarciron
Département Pharmaceutique de Parasitologie et Mycologie Médicale, Faculté de Pharmacie, Université Claude-Bernard, F-69373 Lyon Cedex 08, France
Search for more papers by this authorRoselyne Mancassola
INRA, Laboratoire de Pathologie Aviaire et de Parasitologie, Nouzilly, France
Search for more papers by this authorMuriel Naciri
INRA, Laboratoire de Pathologie Aviaire et de Parasitologie, Nouzilly, France
Search for more papers by this authorAnne-Françoise Petavy
Département Pharmaceutique de Parasitologie et Mycologie Médicale, Faculté de Pharmacie, Université Claude-Bernard, F-69373 Lyon Cedex 08, France
Search for more papers by this authorCorresponding Author
Philippe Lawton
Département Pharmaceutique de Parasitologie et Mycologie Médicale, Faculté de Pharmacie, Université Claude-Bernard, F-69373 Lyon Cedex 08, France
*Corresponding author. Tel.: +33 4 78 77 72 77; Fax: +33 4 78 77 71 58, E-mail address: [email protected]Search for more papers by this authorCarine Hejl
Département Pharmaceutique de Parasitologie et Mycologie Médicale, Faculté de Pharmacie, Université Claude-Bernard, F-69373 Lyon Cedex 08, France
Search for more papers by this authorMarie-Elisabeth Sarciron
Département Pharmaceutique de Parasitologie et Mycologie Médicale, Faculté de Pharmacie, Université Claude-Bernard, F-69373 Lyon Cedex 08, France
Search for more papers by this authorRoselyne Mancassola
INRA, Laboratoire de Pathologie Aviaire et de Parasitologie, Nouzilly, France
Search for more papers by this authorMuriel Naciri
INRA, Laboratoire de Pathologie Aviaire et de Parasitologie, Nouzilly, France
Search for more papers by this authorAnne-Françoise Petavy
Département Pharmaceutique de Parasitologie et Mycologie Médicale, Faculté de Pharmacie, Université Claude-Bernard, F-69373 Lyon Cedex 08, France
Search for more papers by this authorAbstract
The in vitro cultivation of Cryptosporidium parvum in the non-adherent cell line THP-1 was evaluated for its capability as a useful additional model to investigate the effect of drugs on this parasite. The purine analog antiviral 2′,3′-dideoxyinosine (ddI) was evaluated and compared to the reference molecule paromomycin in sequential 24 hour experiments beginning at 24 and 72 hour post-infection. The ability of this technique to evaluate the various parasite stages showed that ddI displayed a dose-dependent efficacy especially on the trophozoite and sexual stages. Paromomycin displayed a lower efficacy than previously reported. Both drugs induced a decrease in the number of multiparasitized cells. These results indicate that the purine salvage pathway should be a key chemotherapeutic target against C. parvum.
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