Cloning and characterization of a new exo-cellulase gene, cel3, in Irpex lacteus
Naoko Hamada
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda 386-8567, Japan
Search for more papers by this authorNaoki Fuse
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda 386-8567, Japan
Search for more papers by this authorMakoto Shimosaka
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda 386-8567, Japan
Search for more papers by this authorRitsuko Kodaira
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda 386-8567, Japan
Search for more papers by this authorYoshihiko Amano
Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University, 500 Wakasato, Nagano 380-8553, Japan
Search for more papers by this authorTakahisa Kanda
Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University, 500 Wakasato, Nagano 380-8553, Japan
Search for more papers by this authorCorresponding Author
Mitsuo Okazaki
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda 386-8567, Japan
Gene Research Center, Shinshu University, 3-15-1 Tokida, Ueda 386-8567, Japan
*Corresponding author. Tel.: +81 (268) 215340; Fax: +81 (268) 215331; E-mail: [email protected]Search for more papers by this authorNaoko Hamada
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda 386-8567, Japan
Search for more papers by this authorNaoki Fuse
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda 386-8567, Japan
Search for more papers by this authorMakoto Shimosaka
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda 386-8567, Japan
Search for more papers by this authorRitsuko Kodaira
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda 386-8567, Japan
Search for more papers by this authorYoshihiko Amano
Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University, 500 Wakasato, Nagano 380-8553, Japan
Search for more papers by this authorTakahisa Kanda
Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University, 500 Wakasato, Nagano 380-8553, Japan
Search for more papers by this authorCorresponding Author
Mitsuo Okazaki
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda 386-8567, Japan
Gene Research Center, Shinshu University, 3-15-1 Tokida, Ueda 386-8567, Japan
*Corresponding author. Tel.: +81 (268) 215340; Fax: +81 (268) 215331; E-mail: [email protected]Search for more papers by this authorAbstract
A new cellulose-inducible gene (named cel3) was isolated from a strain of the white rot basidiomycete, Irpex lacteus MC-2. The cel3 open reading frame, containing two introns, encodes a polypeptide of 526 amino acids residues with a molecular mass of 55 794 Da. Expression of the cel3 gene was induced by various insoluble celluloses and CM-cellulose. Transcription of cel3 was abolished when cells were cultivated in media containing the above cellulosic substrates, but added with glucose, fructose or lactose, while addition of glycerol or mannitol did not affect the cel3 mRNA level. The amino acid sequence of the catalytic domain of the Cel3 protein was homologous to that of fungal exo-type cellulases belonging to family 7 of the glycosyl hydrolases. A phylogenetic study showed that these exo-type cellulases can be clearly separated from family 7 endo-type cellulases.
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