Cell wall structural divergence among Thermus spp.

José Carlos Quintela

Centro de Biologı́a Molecular “Severo Ochoa”, Consejo Superior de Investigaciones Cientı́ficas-Universidad Autónoma de Madrid, Facultad de Ciencias, Campus de Cantoblanco, 28049 Madrid, Spain

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Peter Zöllner,

Peter Zöllner

Institute for Analytical Chemistry, University of Vienna, Währinger Strasse 38, A-1090 Vienna, Austria

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Francisco Garcı́a-del Portillo,

Francisco Garcı́a-del Portillo

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Günter Allmaier,

Günter Allmaier

Institute for Analytical Chemistry, University of Vienna, Währinger Strasse 38, A-1090 Vienna, Austria

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Miguel A de Pedro,

Corresponding Author

Miguel A de Pedro

*Corresponding author. Tel.: +34 (1) 3978083; Fax: +34 (1) 3978087; E-mail: [email protected]Search for more papers by this author

José Carlos Quintela,

José Carlos Quintela

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Peter Zöllner,

Peter Zöllner

Institute for Analytical Chemistry, University of Vienna, Währinger Strasse 38, A-1090 Vienna, Austria

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Francisco Garcı́a-del Portillo,

Francisco Garcı́a-del Portillo

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Günter Allmaier,

Günter Allmaier

Institute for Analytical Chemistry, University of Vienna, Währinger Strasse 38, A-1090 Vienna, Austria

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Miguel A de Pedro,

Corresponding Author

Miguel A de Pedro

*Corresponding author. Tel.: +34 (1) 3978083; Fax: +34 (1) 3978087; E-mail: [email protected]Search for more papers by this author

First published: 17 January 2006

https://doi.org/10.1111/j.1574-6968.1999.tb13472.x

Citations: 3

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Abstract

The fine structure of sacculi from Thermus thermophilus HB27, T. aquaticus YT-1 and Thermus ATCC27737 has been worked out by HPLC analysis and mass spectrometry techniques. The three microorganisms have a murein composition of the rare A3β chemotype, but showed substantial differences in muropeptide composition. Phenylacetylated muropeptides, previously described in T. thermophilus HB8, were detected exclusively in T. thermophilus HB27. Murein from T. aquaticus YT-1 was devoid of d-Ala-d-Ala terminated muropeptides, which were, in contrast, abundant in T. thermophilus HB27 and Thermus ATCC27737. The significance of these findings is discussed.

References

1 Höltje, J.-V. and Schwarz, U. (1985) Biosynthesis and growth of the murein sacculus. In: Molecular Cytology of E. coli (Nanninga, N., Ed.), pp. 77–119. Academic Press, London.
Google Scholar
2 Quintela, J.C., Caparrós, M. and de Pedro, M.A. (1995) Variability of peptidoglycan structural parameters in Gram-negative bacteria. FEMS Microbiol. Lett. 125, 95–100.
10.1111/j.1574-6968.1995.tb07341.x
CAS PubMed Web of Science® Google Scholar
3 Schleifer, K.H. and Kandler, O. (1972) Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol. Rev. 36, 407–477.
10.1128/br.36.4.407-477.1972
CAS PubMed Web of Science® Google Scholar
4 Brock, T.D. (1984) Genus Thermus Brock and Freeze 1969, 295^AL. In: Bergey's Manual of Systematic Bacteriology (Krieg, N.R. and Holt, J.G., Eds.), Vol. 1, pp. 333–337. The Williams and Wilkins Co., Baltimore, MD.
Google Scholar
5 Hensel, R., Demhanter, O., Kandler, R., Kroppensted, R.M. and Stakenbrand, E. (1986) Chemotaxonomic and molecular genetic studies of the genus Thermus: evidence for a phylogenetic relationship of Thermus aquaticus and Thermus ruber to the genus Deinococcus. Int. J. Syst. Bacteriol. 160, 143–152.
Google Scholar
6 Quintela, J.C., Pittenauer, E., Allmaier, G., Arán, V. and de Pedro, M.A. (1995) Structure of peptidoglycan from Thermus thermophilus HB8. J. Bacteriol. 177, 4947–4962.
10.1128/jb.177.17.4947-4962.1995
CAS PubMed Web of Science® Google Scholar
7 Quintela, J.C., Garcı́a-del Portillo, F., Pittenauer, E., Allmaier, G. and de Pedro, M.A. (1999) Peptidoglycan fine structure of the radiotolerant bacterium Deinococcus radiodurans Sark. J. Bacteriol., in press.
Google Scholar
8 Koyama, Y., Hoshino, T., Tomizuka, N. and Furukawa, K. (1986) Genetic transformation of the extreme thermophile Thermus thermophilus and of other Thermus spp. J. Bacteriol. 166, 338–340.
10.1128/jb.166.1.338-340.1986
CAS PubMed Web of Science® Google Scholar
9 Lasa, I., Castón, J.R., Fernández-Herrero, L.A., de Pedro, M.A. and Berenguer, J. (1992) Insertional mutagenesis in the extreme thermophilic eubacteria Thermus thermophilus HB8. Mol. Microbiol. 6, 1555–1564.
10.1111/j.1365-2958.1992.tb00877.x
CAS PubMed Web of Science® Google Scholar
10 Glauner, B. (1988) Separation and quantification of muropeptides with high-performance liquid chromatography. Anal. Biochem. 172, 451–464.
10.1016/0003-2697(88)90468-X
CAS PubMed Web of Science® Google Scholar
11 Glauner, B., Höltje, J.-V. and Schwarz, U. (1988) The composition of the murein of Escherichia coli. J. Biol. Chem. 263, 10088–10095.
CAS PubMed Web of Science® Google Scholar
12 Pfanzagl, B., Zenker, A., Pittenauer, E., Allmaier, G., Martı́nez-Torrecuadrada, J., Schmid, E.R., de Pedro, M.A. and Löffelhardt, W. (1996) Primary structure of cyanelle peptidoglycan of Cyanophora paradoxa: a prokaryotic cell wall as part of an organelle envelope. J. Bacteriol. 178, 332–339.
Google Scholar
13 Vorm, O., Roepstorff, P. and Mann, M. (1994) Improved resolution and very high sensitivity in MALDI TOF of matrix surfaces made by fast evaporation. Anal. Chem. 66, 3281–3287.
10.1021/ac00091a044
CAS Web of Science® Google Scholar
14 Markiewicz, Z., Glauner, B. and Schwarz, U. (1983) Murein structure and lack of DD- and LD-carboxypeptidase activities in Caulobacter crescentus. J. Bacteriol. 156, 649–655.
10.1128/jb.156.2.649-655.1983
CAS PubMed Web of Science® Google Scholar
15 Van Heijenoort, J. (1994) Biosynthesis of the bacterial peptidoglycan unit. In: Bacterial Cell Wall (Ghuysen, J.-M. and Hakenbeck, R., Eds.), pp. 39–71. Elsevier Science Publisher B.V., Amsterdam.
Google Scholar

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Volume172, Issue2

March 1999

Pages 223-229

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Cell wall structural divergence among Thermus spp.

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