Infant gut microbiota is protective against cow's milk allergy in mice despite immature ileal T-cell response
Bertrand Rodriguez
Faculté des Sciences Pharmaceutiques et Biologiques, EA 4065, Département Périnatalité, Microbiologie, Médicament, Université Paris Descartes, Sorbonne Paris Cité, Paris, France
Search for more papers by this authorFeriel Hacini-Rachinel
Nestlé Research Center, Lausanne, Switzerland
Search for more papers by this authorCatherine Ngom-Bru
Nestlé Research Center, Lausanne, Switzerland
Search for more papers by this authorChantal Labellie
Faculté des Sciences Pharmaceutiques et Biologiques, EA 4065, Département Périnatalité, Microbiologie, Médicament, Université Paris Descartes, Sorbonne Paris Cité, Paris, France
Search for more papers by this authorIoannis Nicolis
EA 4466 et Département de Santé publique et Biostatistiques, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris Cité, Paris, France
Search for more papers by this authorMarie-José Butel
Faculté des Sciences Pharmaceutiques et Biologiques, EA 4065, Département Périnatalité, Microbiologie, Médicament, Université Paris Descartes, Sorbonne Paris Cité, Paris, France
Search for more papers by this authorCorresponding Author
Anne-Judith Waligora-Dupriet
Faculté des Sciences Pharmaceutiques et Biologiques, EA 4065, Département Périnatalité, Microbiologie, Médicament, Université Paris Descartes, Sorbonne Paris Cité, Paris, France
Correspondence: Anne-Judith Waligora-Dupriet, EA4065, Ecosystème Intestinal, Probiotiques, Antibiotiques, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, 4 avenue de l'Observatoire, 75006 Paris, France. Tel.: 00 33 (1) 53 73 99 20; fax: 00 33 (1) 53 73 99 23; e-mail: [email protected]Search for more papers by this authorBertrand Rodriguez
Faculté des Sciences Pharmaceutiques et Biologiques, EA 4065, Département Périnatalité, Microbiologie, Médicament, Université Paris Descartes, Sorbonne Paris Cité, Paris, France
Search for more papers by this authorFeriel Hacini-Rachinel
Nestlé Research Center, Lausanne, Switzerland
Search for more papers by this authorCatherine Ngom-Bru
Nestlé Research Center, Lausanne, Switzerland
Search for more papers by this authorChantal Labellie
Faculté des Sciences Pharmaceutiques et Biologiques, EA 4065, Département Périnatalité, Microbiologie, Médicament, Université Paris Descartes, Sorbonne Paris Cité, Paris, France
Search for more papers by this authorIoannis Nicolis
EA 4466 et Département de Santé publique et Biostatistiques, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Sorbonne Paris Cité, Paris, France
Search for more papers by this authorMarie-José Butel
Faculté des Sciences Pharmaceutiques et Biologiques, EA 4065, Département Périnatalité, Microbiologie, Médicament, Université Paris Descartes, Sorbonne Paris Cité, Paris, France
Search for more papers by this authorCorresponding Author
Anne-Judith Waligora-Dupriet
Faculté des Sciences Pharmaceutiques et Biologiques, EA 4065, Département Périnatalité, Microbiologie, Médicament, Université Paris Descartes, Sorbonne Paris Cité, Paris, France
Correspondence: Anne-Judith Waligora-Dupriet, EA4065, Ecosystème Intestinal, Probiotiques, Antibiotiques, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, 4 avenue de l'Observatoire, 75006 Paris, France. Tel.: 00 33 (1) 53 73 99 20; fax: 00 33 (1) 53 73 99 23; e-mail: [email protected]Search for more papers by this authorAbstract
Faecal microbiota of healthy infant displays a large abundance of Bifidobacterium spp. and Bacteroides spp. Although some studies have reported an association between these two genera and allergy, these findings remain a subject of debate. Using a gnotobiotic mouse model of cow's milk allergy, we investigated the impact of an infant gut microbiota – mainly composed of Bifidobacterium and Bacteroides spp. – on immune activation and allergic manifestations. The transplanted microbiota failed to restore an ileal T-cell response similar to the one observed in conventional mice. This may be due to the low bacterial translocation into Peyer's patches in gnotobiotic mice. The allergic response was then monitored in germ-free, gnotobiotic, and conventional mice after repeated oral sensitization with whey proteins and cholera toxin. Colonized mice displayed a lower drop of rectal temperature upon oral challenge with β-lactoglobulin, lower plasma mMCP-1, and lower anti-BLG IgG1 than germ-free mice. The foxp3 gene was highly expressed in the ileum of both colonized mice that were protected against allergy. This study is the first demonstration that a transplanted healthy infant microbiota mainly composed of Bifidobacterium and Bacteroides had a protective impact on sensitization and food allergy in mice despite altered T-cell response in the ileum.
Supporting Information
| Filename | Description |
|---|---|
| fem1207-sup-0001-TableS1.docWord document, 69.5 KB | Table S1. Relative abundance (%) of identified sequences at the level of phylum, genus, and operational taxonomic unit (OTU) in infant stool (I) and mouse faeces (MF) for gnotobiotic groups at day 16. |
| fem1207-sup-0002-TableS2.docWord document, 32.5 KB | Table S2. Immunological analysis at day 15 of Peyer's patches (PP) and mesenteric lymph nodes (MLN) T cells from germ-free, gnotobiotic, and conventional mice. |
| fem1207-sup-0003-TableS3.docWord document, 82 KB | Table S3. Relative abundance (%) of identified sequences at phylum, genus, and operational taxonomic unit (OTU) levels in caecal content of low-responding mice (LR; score 0) and high-responding mice (HR1: score 6; HR2: score 8) to β-lactoglobulin challenge at day 51. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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