Volume 53, Issue 2 pp. 364-368

Dual Fingerprint Reagents with Enhanced Sensitivity: 5-Methoxy- and 5-Methylthioninhydrin

Joseph Almog Ph.D.

Joseph Almog Ph.D.

Casali Institute of Applied Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.

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Asne Klein M.Sc.

Asne Klein M.Sc.

Casali Institute of Applied Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.

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Inbal Davidi M.Sc.

Inbal Davidi M.Sc.

Casali Institute of Applied Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.

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Yaron Cohen M.Sc.

Yaron Cohen M.Sc.

Latent Fingerprint Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police, National H.Q., Jerusalem 91906, Israel.

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Myriam Azoury M.Sc.

Myriam Azoury M.Sc.

Latent Fingerprint Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police, National H.Q., Jerusalem 91906, Israel.

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Michal Levin-Elad Ph.D.

Michal Levin-Elad Ph.D.

Latent Fingerprint Laboratory, Division of Identification and Forensic Science (DIFS), Israel Police, National H.Q., Jerusalem 91906, Israel.

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First published: 24 March 2008
Citations: 17
Additional information and reprint requests:
Joseph Almog, Ph.D.
Professor of Forensic Chemistry
Casali Institute of Applied Chemistry
The Hebrew University of Jerusalem
Jerusalem 91904
Israel
E-mail: [email protected]

Abstract

Abstract: “Dual fingerprint reagents” are chemical formulations which produce with latent fingerprints in a single step, impressions that are both colored and fluorescent. Pre-mixed solutions of the two commercially available ninhydrin analogues, 5-methoxyninhydrin (MN) and 5-methylthioninhydrin (MTN) with zinc or cadmium salts, are true dual reagents. They are much more sensitive than the parent dual reagent, ninhydrin/ZnCl2. The main advantage of the new formulations is that they can be used at room temperature, with no need to cool the sample to liquid nitrogen temperature. At 0.05% concentration, which is 10-fold lower than the common ninhydrin working solution, MTN/ZnCl2 is as sensitive as DFO in the fluorescence mode and considerably more sensitive in the color mode. MTN is also slightly cheaper than DFO.

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