Molecular Cloning and Characterization of a Superoxide Dismutase (sod) Gene in Pneumocystis carinii
Corresponding Author
Cecile-Marie Denis
INSERM U42, 369, rue Jules Guesde, B. P. 39. 59651 Villeneuve d'Ascq Ceclex, France
Faculty of Science. 13. rue de Tout. 59000 Lille, France
Molecular Infectious Diseases Group. Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington. Oxford OX3 9DU England.
To whom correspondence should be addressed. Telephone: 1-865-22-23-44; Fax: 1-865-22-26-26, Email: [email protected]Search for more papers by this authorKarine Guyot
INSERM U42, 369, rue Jules Guesde, B. P. 39. 59651 Villeneuve d'Ascq Ceclex, France
Department of Ecosystem Microbiology, Pasteur Institute of Lille. 59019 Lille. France
Search for more papers by this authorAnn E. Wakefield
Molecular Infectious Diseases Group. Institute of Molecular Medicine, Oxford, UK
Search for more papers by this authorDaniel Dive
INSERM U42, 369, rue Jules Guesde, B. P. 39. 59651 Villeneuve d'Ascq Ceclex, France
Search for more papers by this authorEduardo Dei-Cas
Faculty of Medicine, Regional Hospital Center, I. place de Verdun. 59X50 Lille, France
Search for more papers by this authorDaniel Camus
INSERM U42, 369, rue Jules Guesde, B. P. 39. 59651 Villeneuve d'Ascq Ceclex, France
Search for more papers by this authorCarmen Odberg-Ferragut
INSERM U42, 369, rue Jules Guesde, B. P. 39. 59651 Villeneuve d'Ascq Ceclex, France
Search for more papers by this authorCorresponding Author
Cecile-Marie Denis
INSERM U42, 369, rue Jules Guesde, B. P. 39. 59651 Villeneuve d'Ascq Ceclex, France
Faculty of Science. 13. rue de Tout. 59000 Lille, France
Molecular Infectious Diseases Group. Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington. Oxford OX3 9DU England.
To whom correspondence should be addressed. Telephone: 1-865-22-23-44; Fax: 1-865-22-26-26, Email: [email protected]Search for more papers by this authorKarine Guyot
INSERM U42, 369, rue Jules Guesde, B. P. 39. 59651 Villeneuve d'Ascq Ceclex, France
Department of Ecosystem Microbiology, Pasteur Institute of Lille. 59019 Lille. France
Search for more papers by this authorAnn E. Wakefield
Molecular Infectious Diseases Group. Institute of Molecular Medicine, Oxford, UK
Search for more papers by this authorDaniel Dive
INSERM U42, 369, rue Jules Guesde, B. P. 39. 59651 Villeneuve d'Ascq Ceclex, France
Search for more papers by this authorEduardo Dei-Cas
Faculty of Medicine, Regional Hospital Center, I. place de Verdun. 59X50 Lille, France
Search for more papers by this authorDaniel Camus
INSERM U42, 369, rue Jules Guesde, B. P. 39. 59651 Villeneuve d'Ascq Ceclex, France
Search for more papers by this authorCarmen Odberg-Ferragut
INSERM U42, 369, rue Jules Guesde, B. P. 39. 59651 Villeneuve d'Ascq Ceclex, France
Search for more papers by this authorAbstract
This work reports the isolation and characterization of a gene encoding a superoxide dismutase (SOD. EC.1.15.1.1.) from Pneumocystis carinii derived from rat. Sense and antisense oligonucleotides, deduced from SOD amino acid sequences from a wide variety of organisms, allowed amplification of a 669 bp genomic DNA fragment specific to this P. carinii. RACE-PCR was used to obtain the major pan of the complementary DNA; the 5- and 3′-genomic regions were obtained respectively from a Mbo I subgenomic library and from an amplified fragment using oligonucleotides designed from the cDNA sequence. Comparison of genomic and cDNA sequences showed an open reading frame of 660 bp interrupted by seven small introns. The deduced amino acid sequence contained 220 residues. Protein sequence alignment demonstrated the highest homology (50.5% identity. 70.3% similarity) with Saccharomyces cerevisiae manganese-SOD (MnSOD) suggesting that P. carinii SOD belongs to the mitochondrial MnSOD group. A putative targeting peptide found at the 5′-end of the P. carinii SOD sequence also suggested its mitochondrial localization.
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