Full Access
Identification of Antigens Specific for Pneumocystis carinii
JOSEPH A. KOVACS,
BETTINA LUNDGREN,
HENRY MASUR,
JOSEPH A. KOVACS
Critical Core Medicine Deportment, Notional Institutes of Health, Bethesda, Moryland 20892.
Search for more papers by this authorBETTINA LUNDGREN
Critical Core Medicine Deportment, Notional Institutes of Health, Bethesda, Moryland 20892.
Search for more papers by this authorHENRY MASUR
Critical Core Medicine Deportment, Notional Institutes of Health, Bethesda, Moryland 20892.
Search for more papers by this authorJOSEPH A. KOVACS,
BETTINA LUNDGREN,
HENRY MASUR,
JOSEPH A. KOVACS
Critical Core Medicine Deportment, Notional Institutes of Health, Bethesda, Moryland 20892.
Search for more papers by this authorBETTINA LUNDGREN
Critical Core Medicine Deportment, Notional Institutes of Health, Bethesda, Moryland 20892.
Search for more papers by this authorHENRY MASUR
Critical Core Medicine Deportment, Notional Institutes of Health, Bethesda, Moryland 20892.
Search for more papers by this author
LITERATURE CITED
- 1 Galfre, G. & Milslein, C. 1981. Preparation of monoclonal antibodies: strategies and procedures. Method. Enzymol., 73: 3–46.
- 2 Gigliotti, F., Stokes, D. C., Cheatham, A. B. et al. 1986. Development of murive moncclonal antibodies to Pneumocystis carinii. J. Infect. Dis., 154: 315–321.
- 3 Gill, V. J., Evans, G., Stock, F, Parrillo, J. E., Masur, H. & Kovacs, J. A. 1987. Detection of Pneumocystis carinii by fluorescent antibody stain using a combination of three monoclonal antibodies. J. Clin. Microbiol., 25: 1837–1840.
- 4 Kim, H. K., Hughes, W. T., & Fcldman, S. 1972. Studies of morphology and immunofluorescence of Pneumocystis carinii. Proc. Soc. Exp. Biol. Med., 141: 304–309.
- 5 Kovacs, J. A., Halpcm, J., Swan, J. C., Moss, J., Parrillo, J. E. & Masur, H. 1988. Identification of antigens and antibodies specific for Pneumocystis carinii. J. Immunol., 140: 2023–2031.
- 6 Kovacs, J. A., Hicmenz, J. W., Macher, A. M., et al. 1984. Pneumocystis carinii pneumonia: a comparison between patients with the acquired immunodeficiency syndrome and patients with other immunodeficiencies. Ann. Intern. Med., 100: 663–671.
- 7 Kovacs, J. A., Ng. V., Masur, H., Leoung, G., Hadley, W. K., Evans, G, Lane, H. C., Parrillo, J. E. & Gill, V. J 1988. Diagnosis of Pneumocystis carinii pneumonia: improved detection in sputum using monoclonal antibodies. N. Engl. J. Med., 318: 589–593.
- 8 Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of the bacteriophage T-4. Nature (London), 227: 680–685.
- 9 Towbin, H., Stachlin, T. & Gordon, J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA, 76: 4350–4354.
- 10 Walzcr, P. D. & Linke, M. J. 1987. A comparison of the antigenic characteristics of rat and human Pneumocystis carinii by immunoblotting. J. Immunol., 138: 2257–2265.
- 11 Walzcr, P. D. & Rutledge, M. E. 1980. Comparison of rat, mouse, and human Pneumocystis carinii by immunofluorescence. J. Infect. Dis., 142: 449.
