The Cell Cycle in Crithidia fasciculata. Temporal Relationships between Synthesis of Deoxyribonucleic Acid in the Nucleus and in the Kinetoplast1, 2
WILLIAM B. COSGROVE
Dept. of Zoology, Univ. of Georgia, Athens, Ga. 30601
Search for more papers by this authorMARIANNE J. SKEEN
Dept. of Zoology, Univ. of Georgia, Athens, Ga. 30601
Yerkes Regional Primate Research Center, Emory Univ., Atlanta, Georgia 30322. A portion of this material was submitted in partial fulfillment of the requirements for the M. S. degree.
Search for more papers by this authorWILLIAM B. COSGROVE
Dept. of Zoology, Univ. of Georgia, Athens, Ga. 30601
Search for more papers by this authorMARIANNE J. SKEEN
Dept. of Zoology, Univ. of Georgia, Athens, Ga. 30601
Yerkes Regional Primate Research Center, Emory Univ., Atlanta, Georgia 30322. A portion of this material was submitted in partial fulfillment of the requirements for the M. S. degree.
Search for more papers by this authorSupported in part by NIH grant AI 08509 to WBC.
The following abbreviations are used: 3H-TdR = thymidinemethyl-3H = tritiated thymidine; t = time in minutes; T = generation time in minutes. The subscripts k and n refer to kinetoplast and nucleus.
Abstract
SYNOPSIS. Autoradiographic technics with tritium-labeled thymidine have been used to determine G1, S, G2 and D for the kinetoplast and the nucleus of Crithidia fasciculata at 15, 25 and 32 C. The kinetoplast completes division before the nucleus at all 3 temperatures. The S phases of both organelles occur in approximate synchrony and are approximately equal in length but the nucleus begins and completes S before the kinetoplast at the 2 lower temperatures. This relationship is reversed at 32 C. Most of the effect of temperature on generation time is due to its effect on the length of S.
The results are compared with similar studies on C. luciliae, Trypanosoma mega, other protozoa and tissue cells in culture. The role of the approximate synchrony of nuclear and kinetoplastic cycles in maintenance of the kinetoplastic condition is discussed and the hypothesis is proposed that this synchrony results from the sharing by nucleus and kinetoplast of the same mechanism for the production of the deoxyribonucleotides used in replication of their respective DNAs.
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