Volume 47, Issue 7 pp. 1241-1249

Short-term liquid storage of CD14+ monocytes, CD11c+, and CD123+ precursor dendritic cells produced by leukocytapheresis

Erwin F. Strasser

Erwin F. Strasser

From the Transfusion Medicine and Hemostasis Department, University Hospital Erlangen, Erlangen, Germany.

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Benjamin Keller

Benjamin Keller

From the Transfusion Medicine and Hemostasis Department, University Hospital Erlangen, Erlangen, Germany.

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Martin Hendelmeier

Martin Hendelmeier

From the Transfusion Medicine and Hemostasis Department, University Hospital Erlangen, Erlangen, Germany.

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Jürgen Ringwald

Jürgen Ringwald

From the Transfusion Medicine and Hemostasis Department, University Hospital Erlangen, Erlangen, Germany.

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Jürgen Zingsem

Jürgen Zingsem

From the Transfusion Medicine and Hemostasis Department, University Hospital Erlangen, Erlangen, Germany.

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Reinhold Eckstein

Reinhold Eckstein

From the Transfusion Medicine and Hemostasis Department, University Hospital Erlangen, Erlangen, Germany.

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First published: 14 May 2007
Citations: 7
Erwin F. Strasser, MD, PhD, Transfusion Medicine and Hemostaseology Department, University Hospital of Erlangen, Krankenhausstrasse 12, D-91054 Erlangen, Germany; e-mail: [email protected].

This work was supported in part by a grant from the ELAN-Program (“Fond fuer Forschung und Lehre”) of the University Hospital Erlangen.

Abstract

BACKGROUND: This prospective study compared white blood cell (WBC) storage in polyvinylchloride (PVC) bags and in polyolefin (POF) bags. After leukapheresis, CD14+ monocytes, CD11c+, and CD123+ precusor dendritic cells (DCs) were analyzed under platelet (PLT) storage conditions.

STUDY DESIGN AND METHODS: Twenty-five leukapheresis procedures were performed on blood cell separators (AS.TEC204 [PVC; Fresenius HemoCare GmbH] and the COBE Spectra [POF, Gambro BCT]). Blood cell counts, glucose, lactic acid, pO2, pCO2, and pH were measured in mononuclear cell (MNC) harvests on Days 0, 1, 3, and 5. WBCs were analyzed by flow cytometry.

RESULTS: The WBC yields of the AS.TEC204 harvests were 25 percent higher (13.4 × 109 ± 2.7 × 109 WBCs) compared to the COBE Spectra (9.9 × 109 ± 2.5 × 109 WBCs). During 5-day storage, WBC counts (PVC bags) decreased significantly (24%). Storage in POF bags showed more consistent results (WBC loss, 6%). Loss of CD14+ monocytes and CD11c+ precursor DCs did not differ significantly in leukapheresis products. CD123+ precursor DCs stored in PVC bags dropped by more than 90 percent (POF bags, 24%). Lactic acid concentrations exceeded 20 mmol per L after 24 hours in PVC bags and after 72 hours in POF bags. The mean pH value on Day 5 was 6.2 (PVC) and 6.3 (POF). On Day 1, the product glucose concentration decreased by 76 percent after storage in PVC bags and by 16 percent in POF bags.

CONCLUSIONS: Storage of MNCs within 72 hours in the original harvest container assures stable WBC content and is easy to perform. POF bags should be preferred in the case of extended WBC storage. Patient studies should clarify changes in efficiency of hematopoietic reconstitution that might occur over time during MNC storage.

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