Profiling of alterations in platelet proteins during storage of platelet concentrates
Thomas Thiele
From the Institute for Immunology and Transfusion Medicine; Interfaculty Institute for Genetics and Functional Genomics; Department of Otorhinolaryngology, Head and Neck Surgery, Ernst-Moritz-Arndt University, Greifswald, Germany; and the Theodor Kocher Institute, Universität Bern, Bern, Switzerland.
Search for more papers by this authorLeif Steil
From the Institute for Immunology and Transfusion Medicine; Interfaculty Institute for Genetics and Functional Genomics; Department of Otorhinolaryngology, Head and Neck Surgery, Ernst-Moritz-Arndt University, Greifswald, Germany; and the Theodor Kocher Institute, Universität Bern, Bern, Switzerland.
Search for more papers by this authorSimon Gebhard
From the Institute for Immunology and Transfusion Medicine; Interfaculty Institute for Genetics and Functional Genomics; Department of Otorhinolaryngology, Head and Neck Surgery, Ernst-Moritz-Arndt University, Greifswald, Germany; and the Theodor Kocher Institute, Universität Bern, Bern, Switzerland.
Search for more papers by this authorChristian Scharf
From the Institute for Immunology and Transfusion Medicine; Interfaculty Institute for Genetics and Functional Genomics; Department of Otorhinolaryngology, Head and Neck Surgery, Ernst-Moritz-Arndt University, Greifswald, Germany; and the Theodor Kocher Institute, Universität Bern, Bern, Switzerland.
Search for more papers by this authorElke Hammer
From the Institute for Immunology and Transfusion Medicine; Interfaculty Institute for Genetics and Functional Genomics; Department of Otorhinolaryngology, Head and Neck Surgery, Ernst-Moritz-Arndt University, Greifswald, Germany; and the Theodor Kocher Institute, Universität Bern, Bern, Switzerland.
Search for more papers by this authorMatthias Brigulla
From the Institute for Immunology and Transfusion Medicine; Interfaculty Institute for Genetics and Functional Genomics; Department of Otorhinolaryngology, Head and Neck Surgery, Ernst-Moritz-Arndt University, Greifswald, Germany; and the Theodor Kocher Institute, Universität Bern, Bern, Switzerland.
Search for more papers by this authorNorbert Lubenow
From the Institute for Immunology and Transfusion Medicine; Interfaculty Institute for Genetics and Functional Genomics; Department of Otorhinolaryngology, Head and Neck Surgery, Ernst-Moritz-Arndt University, Greifswald, Germany; and the Theodor Kocher Institute, Universität Bern, Bern, Switzerland.
Search for more papers by this authorKenneth J. Clemetson
From the Institute for Immunology and Transfusion Medicine; Interfaculty Institute for Genetics and Functional Genomics; Department of Otorhinolaryngology, Head and Neck Surgery, Ernst-Moritz-Arndt University, Greifswald, Germany; and the Theodor Kocher Institute, Universität Bern, Bern, Switzerland.
Search for more papers by this authorUwe Völker
From the Institute for Immunology and Transfusion Medicine; Interfaculty Institute for Genetics and Functional Genomics; Department of Otorhinolaryngology, Head and Neck Surgery, Ernst-Moritz-Arndt University, Greifswald, Germany; and the Theodor Kocher Institute, Universität Bern, Bern, Switzerland.
Search for more papers by this authorAndreas Greinacher
From the Institute for Immunology and Transfusion Medicine; Interfaculty Institute for Genetics and Functional Genomics; Department of Otorhinolaryngology, Head and Neck Surgery, Ernst-Moritz-Arndt University, Greifswald, Germany; and the Theodor Kocher Institute, Universität Bern, Bern, Switzerland.
Search for more papers by this authorThomas Thiele
From the Institute for Immunology and Transfusion Medicine; Interfaculty Institute for Genetics and Functional Genomics; Department of Otorhinolaryngology, Head and Neck Surgery, Ernst-Moritz-Arndt University, Greifswald, Germany; and the Theodor Kocher Institute, Universität Bern, Bern, Switzerland.
Search for more papers by this authorLeif Steil
From the Institute for Immunology and Transfusion Medicine; Interfaculty Institute for Genetics and Functional Genomics; Department of Otorhinolaryngology, Head and Neck Surgery, Ernst-Moritz-Arndt University, Greifswald, Germany; and the Theodor Kocher Institute, Universität Bern, Bern, Switzerland.
Search for more papers by this authorSimon Gebhard
From the Institute for Immunology and Transfusion Medicine; Interfaculty Institute for Genetics and Functional Genomics; Department of Otorhinolaryngology, Head and Neck Surgery, Ernst-Moritz-Arndt University, Greifswald, Germany; and the Theodor Kocher Institute, Universität Bern, Bern, Switzerland.
Search for more papers by this authorChristian Scharf
From the Institute for Immunology and Transfusion Medicine; Interfaculty Institute for Genetics and Functional Genomics; Department of Otorhinolaryngology, Head and Neck Surgery, Ernst-Moritz-Arndt University, Greifswald, Germany; and the Theodor Kocher Institute, Universität Bern, Bern, Switzerland.
Search for more papers by this authorElke Hammer
From the Institute for Immunology and Transfusion Medicine; Interfaculty Institute for Genetics and Functional Genomics; Department of Otorhinolaryngology, Head and Neck Surgery, Ernst-Moritz-Arndt University, Greifswald, Germany; and the Theodor Kocher Institute, Universität Bern, Bern, Switzerland.
Search for more papers by this authorMatthias Brigulla
From the Institute for Immunology and Transfusion Medicine; Interfaculty Institute for Genetics and Functional Genomics; Department of Otorhinolaryngology, Head and Neck Surgery, Ernst-Moritz-Arndt University, Greifswald, Germany; and the Theodor Kocher Institute, Universität Bern, Bern, Switzerland.
Search for more papers by this authorNorbert Lubenow
From the Institute for Immunology and Transfusion Medicine; Interfaculty Institute for Genetics and Functional Genomics; Department of Otorhinolaryngology, Head and Neck Surgery, Ernst-Moritz-Arndt University, Greifswald, Germany; and the Theodor Kocher Institute, Universität Bern, Bern, Switzerland.
Search for more papers by this authorKenneth J. Clemetson
From the Institute for Immunology and Transfusion Medicine; Interfaculty Institute for Genetics and Functional Genomics; Department of Otorhinolaryngology, Head and Neck Surgery, Ernst-Moritz-Arndt University, Greifswald, Germany; and the Theodor Kocher Institute, Universität Bern, Bern, Switzerland.
Search for more papers by this authorUwe Völker
From the Institute for Immunology and Transfusion Medicine; Interfaculty Institute for Genetics and Functional Genomics; Department of Otorhinolaryngology, Head and Neck Surgery, Ernst-Moritz-Arndt University, Greifswald, Germany; and the Theodor Kocher Institute, Universität Bern, Bern, Switzerland.
Search for more papers by this authorAndreas Greinacher
From the Institute for Immunology and Transfusion Medicine; Interfaculty Institute for Genetics and Functional Genomics; Department of Otorhinolaryngology, Head and Neck Surgery, Ernst-Moritz-Arndt University, Greifswald, Germany; and the Theodor Kocher Institute, Universität Bern, Bern, Switzerland.
Search for more papers by this authorSupport by the Swiss National Science Foundation, Grant 310000-107754 (KJC); Alfried Krupp Wissenschaftskollegs Greifswald (KJC); BMBF-ZIK-FunGene and BMBF/NBL3 [01-ZZ0403] (UV and AG); Landesförderungsprogramm Mecklenburg Vorpommern (EFRE to AG).
Abstract
BACKGROUND: The quality of platelet concentrates (PCs) is primarily determined in vitro by selective methods (e.g., pH, aggregometry), which provide only limited information on certain platelet (PLT) characteristics. In contrast, proteomic technologies provide a comprehensive overview of the PLT proteome. High interassay variability, however, limits meaningful assessment of samples taken from the same product over time or before and after processing.
STUDY DESIGN AND METHODS: Differential in-gel electrophoresis (DIGE) and mass spectrometry were applied to analyze changes in the PLT proteome during storage of PCs.
RESULTS: DIGE provides a comprehensive and reproducible overview of the cytoplasmic PLT proteome (median standard deviation of protein spot intensities, 5%-9%). Although 97 percent of cytosolic PLT proteins remained unchanged over a 9-day storage period, septin 2 showed characteristic alterations that preceded by several days more widespread alterations affecting numerous other proteins. Also β-actin and gelsolin are potential marker proteins for changes in the PLT proteome. Interestingly septin 2 and gelsolin are affected during apoptosis, indicating that apoptosis in PCs may have an impact on PLT storage.
CONCLUSION: DIGE is a tool for comprehensively assessing the impact of storage on the global proteome profile of therapeutic PCs. Most of the changes detected are in high-abundance PLT proteins.
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