QUANTITATIVE GENETIC ANALYSIS OF MORPHOLOGICAL VARIATION IN AN ANTARCTIC DIATOM GROWN AT TWO LIGHT INTENSITIES1
A. Michelle Wood
Department of Biology, The University of Chicago, Chicago, Illinois 60637 and Bigelow Laboratory for Ocean Sciences, West Boothbay Harbor, Maine 04575
Address for reprint requests. The University of Chicago, Department of Biology, 915 East 57 th Street, Chicago, Illinois 60637.
Search for more papers by this authorRussell Lande
Department of Biology, The University of Chicago, Chicago, Illinois 60637 and Bigelow Laboratory for Ocean Sciences, West Boothbay Harbor, Maine 04575
Search for more papers by this authorGreta A. Fryxell
Department of Occeanography, Texas A&M University, College Station, Texas 77843-3146
Search for more papers by this authorA. Michelle Wood
Department of Biology, The University of Chicago, Chicago, Illinois 60637 and Bigelow Laboratory for Ocean Sciences, West Boothbay Harbor, Maine 04575
Address for reprint requests. The University of Chicago, Department of Biology, 915 East 57 th Street, Chicago, Illinois 60637.
Search for more papers by this authorRussell Lande
Department of Biology, The University of Chicago, Chicago, Illinois 60637 and Bigelow Laboratory for Ocean Sciences, West Boothbay Harbor, Maine 04575
Search for more papers by this authorGreta A. Fryxell
Department of Occeanography, Texas A&M University, College Station, Texas 77843-3146
Search for more papers by this authorAccepted: 3 September 1986
ABSTRACT
Ten clonal isolates of Thalassiosira tumida (Janisch) Hasle were grown in duplicate semi-continuous batch cultures at 116 and 11.6 μE.m−2.s−1; acclimated cells were harvested during exponential growth and cleaned for examination by light microscopy (LM) and scanning electron microscopy (SEM). Number of strutted processes surrounding the central annulus (SP) and average number of satellite pores per process (AVSAT) were counted using SEM on 20 valves from each culture grown in high light, for a total of 400 valves examined; number of marginal labiate processes (LP) and overall diameter (DIAM) were measured using LM on 20 valves from each culture grown in both high and low light for a total of 800 valves examined. Univariate analysis of variance showed that bottle effects resulting from microenvironmental differences between replicates were a small but significant source of variation in DIAM, LP, and SP but not AVSAT. Significant differences among clones were observed for all characters. Decreased irradiance resulted in a significant decrease in valve diameter but no significant effect on LP; no light x clone interaction was obsered. Significant covariance between characters among clones was also observed; since valve diameter is known to decrease during asexual growth, the correlation coefficients for SP, AVSAT, and LP with DIAM were used to correct the data for this source of nongenetic differences between clones. Analysis of the size-corrected data showed that the proportion of total phenotypic variance in SP, LP, and AVSAT caused by genetic differences among clones was 0.14, 0.14, and 0.30, respectively. This indicates that the majority of total phenotypic variance was due to environmental or developmental causes, but that sufficient genetic variability exists to support rapid phenotypic evolution in SP, LP, and AVSAT under continued directional selection. Finally, the results of the genetic analysis revealed a high (0.82) genetic correlation between SP and LP.
References
- A. Allen, [Ed.] 1982. SAS User's Guide: Basics. SAS Institute, Cary , 921 pp.
- Arnold, S. J. 1981. Behavioral variation in natural populations. I. Phenotypic, genetic and environmental correlations between chemoreceptive responses to prey in the garter snake, Thamnophis elegans. Evolution 35: 489–509.
- Barton, N. H. & Charlesworth, B. 1984. Genetic revolutions, founder effects, and speciation. Annu. Rev. Ecol. Syst. 15: 133–64.
- Beam, C. A. & Himes, M. 1977. Sexual isolation and genetic diversification among some strains of Crypthecodinium cohnii-like dinoflagellates. Evidence of speciation. J. Protozool. 24: 532–9.
- Beam, C. A. & Himes, M. 1982. Distribution of members of the Crypthecodinium cohnii (Dinophyceae) species complex. J. Protozool. 29: 8–15.
- Brand, L. E. 1981. Genetic variability in reproduction rates in marine phytoplankton populations. Evolution 35: 1117–27.
- Brand, L. E. 1982. Genetic variability and spatial patterns of genetic differentiation in the reproductive rates of the marine coccolithopores Emiliana huxleyi and Gephyrocapsa oceanica. Limnol. Oceanogr. 27: 236–45.
- Brand, L. E. 1985. Low genetic variability in reproduction rates in populations of Prorocentrum micans Ehrenb. (Dinophyceae) over Georges Bank. J. Exp. Mar. Biol. Ecol. 88: 55–65.
-
Brand, L. E.,
Guillard, R. R. L. &
Murphy, L. S.
1981. A method for the rapid and precise determination of acclimated phytoplankton reproduction rates.
J. Plank. Res.
3: 193–201.
10.1093/plankt/3.2.193 Google Scholar
- Dobzhansky, Th. 1970. Genetics of the Evolutionary Process. Columbia Univ. Press, New York , 505 pp.
-
Drebes, G.
1966. On the life history of the marine plankton diatom Stephanopyxis palmeriana.
Helgol. Wiss. Meeresunters
13: 101–4.
10.1007/BF01612659 Google Scholar
- Drebes, G. 1977. Sexuality. In D. Werner [Ed.] The Biology of Diatoms. Univ. California Press, Berkeley , pp. 250–83.
- Falconer, D. S. 1981. Introduction to Quantitative Genetics, 2nd ed. Longman, New York , 340 pp.
- Fisher, N. S., Graham, L. B., Carpenter, E. J. & Wurster, C. F. 1973. Geographic differences in phytoplankton sensitivity to PCBs. Nature (Lond.) 241: 548–9.
-
Fryxell, G. A.
1976. The position of the labiate process in the diatom genus Skeletonema.
Br. Phycol. J.
11: 93–9.
10.1080/00071617600650131 Google Scholar
- Fryxell, G. A. 1983. New evolutionary patterns in diatoms. BioScience 33: 92–8.
- Fryxell, G. A., Doucette, G. J. & Hubbard, G. F. 1981. The genus Thalassiosira: the bipolar diatom T. antarctica Comber. Bot. Mar. 24: 321–35.
- Fryxell, G. A. & Hasle, G. R. 1983. The antarctic diatoms Thalassiosira dichotomica (Kozlova) comb. nov. and T. ambigua Kozlova. Polar Biol. 2: 53–62.
- Fryxell, G. A., Hasle, G. R. & Carty, S. V. 1986. Thalassiosira tumida (Janisch) Hasle: observations from field and clonal cultures. Eighth International Symp. on Recent and Fossil Diatoms, Paris. In press.
- Gallagher, J. C. 1980. Population genetics of Skeletonema costatum (Bacillariophyceae) in Narragansett Bay. J. Phycol. 16: 464–74.
- Gallagher, J. C. 1982. Physiological variation and electrophoretic banding patterns of genetically different seasonal populations of Skeletonema costatum (Bacillariophyceae). J. Phycol. 18: 148–62.
- Gallagher, J. C., Wood, A. M. & Alberte, R. S. 1984. Ecotypic differentiation in the marine diatom Skeletonema costatum: influence of light intensity on the photosynthetic apparatus. Mar. Bid. (Berl.) 82: 121–34.
- Guillard, R. R. L. & Ryther, J. H. 1962. Studies on marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervaces (Cleve) Gran. Can. J. Microbiol. 8: 229–39.
- Hasle, G. R. 1972. Two types of valve processes in centric diatoms. Beih. Nova Hedwigia 39: 55–78.
- Hasle, G. R. 1973a. Morphology and taxonomy of Skeletonema costatum (Bacillariophyceae). Norw. J. Bot. 20: 109–37.
- Hasle, G. R. 1973b. Thalassiosiraceae, a new diatom family. Norw. J. Bot. 20: 67–9.
- Hasle, G. R. & Booth, B. C. 1984. Nitzschia cylindroformis sp. nov., a common and abundant plankton diatom of the eastern subarctic Pacific. J. Plank. Res. 6: 493–503.
- Hasle, G. R. & Fryxell, G. A. 1970. Diatoms: cleaning and mounting for light and electron microscopy. Trans. Am. Microscop. Soc. 89: 469–74.
-
Hasle, G. R.,
Heimdal, B. R. &
Fryxell, G. A.
1971. Morphologic variability in fasiculated diatoms as exemplified by Thalassiosira tumida (Janisch) Hasle, Comb. nov.
Antarct. Res. Ser.
17: 313–33.
10.1029/AR017p0313 Google Scholar
- Hasle, G. R. & Syvertsen, E. E. 1985. Thalassiosiropsis, a new diatom from the fossil records. Micropaleontology 31: 82–91.
- Hendey, N. I. 1959. The structure of the diatom cell wall as revealed by the electron microscope. J. Quekett Micr. 5: 147–75.
- Himes, M. & Beam, C. A. 1975. Genetic analysis in the dinoflagellate Crypthecodinium (Gyrodinium) cohnii. Evidence for unusual meiosis. Proc. Natl. Acad. Sci. U.S.A. 72: 4546–9.
- Johansen, J. R. & Fryxell, G. A. 1985. The genus Thalassiosira (Bacillariophyceae): studies on species occurring south of the Antarctic Convergence Zone. Phycologia 24: 155–79.
- Kendall, M. G. & Stuart, A. 1973. The Advanced Theory of Statistics, Vol. 2. Inference and Relationship, 3rd ed. Hafner, New York , 723 pp.
- Lande, R. 1979. Quantitative genetic analysis of multivariate evolution, applied to brain:body size allometry. Evolution 33: 402–16.
- Lande, R. & Arnold, S. J. 1983. The measurement of selection on correlated characters. Evolution 37: 1210–26.
- Leamy, L. 1974. Heritability of osteometric traits in a randombred population of house mice. J. Heredity 65: 109–20.
- Lewin, J. C. & Guillard, R. R. L. 1963. Diatoms. Annu. Rev. Microbiol. 17: 373–414.
- Lynch, M. 1984. The limits to life history evolution in Daphnia. Evolution 38: 465–82.
- Lynch, M. & Gabriel, W. 1983. Phenotypic evolution and parthenogenesis. Am. Nat. 122: 745–64.
- Mann, D. G. 1984. Observations on copulation in Navicula pupula and Amphora ovalis in relation to the nature of diatom species. Ann. of Bot. 54: 429–38.
- Mayo, O. 1980. The Theory of Plant Breeding. Oxford Univ. Press, Oxford , 293 pp.
- Murphy, L. S. & Belastock, R. A. 1980. The effect of environmental origin on the response of marine diatoms to chemical stress. Limnol Oceanogr. 25: 160–5.
- Murphy, L. S. & Guillard, R. R. L. 1976. Biochemical taxonomy of marine phytoplankton by electrophoresis of enzymes. I. The centric diatoms Thalassiosira pseudonana and T. fluviatilis. J. Phycol. 12: 9–13.
- Nei, M. 1972. Genetic distance between populations. Am. Nat. 106: 283–92.
- Paasche, E. 1975. The influence of salinity on the growth of some plankton diatoms from brackish water. Norw. J. Bot. 22: 209–15.
-
Price, T. D.,
Grant, P. R. &
Boag, P. T.
1984. Genetic changes in the morphological evolution of Darwin's ground finches. In K. Wöhrmann &
V. Loeschcke [Eds.] Population Biology and Evolution. Springer-Verlag,
Berlin
, pp. 49–66.
10.1007/978-3-642-69646-6_5 Google Scholar
- Primack, R. B. & Antonovics, J. 1981. Experimental ecological genetics in Plantago. V. Components of seed yield in the ribwort plantain Plantago lanceolata L. Evolution 35: 1069–79.
- A. A. Ray, [Ed.] 1982. SAS Users Guide: Statistics. SAS Institute, Cary , 584 pp.
- Reeve, E. C. R. & Robertson, F. W. 1954. Studies in quantitative inheritance. IV. Sternite chaeta number in Drosophila: a metameric quantitative character. Z. Indukt. Abstammungs-Vererbungsl. 86: 269–88.
- Reyment, R. A., Blackith, R. E. & Campbell, N. A. 1984. Multivariate Morphometrics, 2nd ed. Academic Press, New York , 233 pp.
- Robertson, A. 1959a. Experimental design in the evaluation of genetic parameters. Biometrics 15: 219–26.
- Robertson, A. 1959b. The sampling variance of the genetic correlation coefficient. Biometrics 15: 469–85.
- Ross, R. & Sims, P. A. 1973. Observations on family and generic limits in the Centrales. Beih. Nova Hedwigia 45: 97–130.
- Round, F. E. 1981. The Ecology of Algae. Cambridge Univ. Press, Cambridge , 653 pp.
- Scheffé, H. 1959. The Analysis of Variance. Wiley, New York , 477 pp.
- Schmid, A.-M. M. 1979. Influence of environmental factors on the development of the valve in diatoms. Protoplasma 99: 99–115.
- Simmonds, N. W. 1979. Principles of Crop Improvement. Longman, New York , 408 pp.
- Simonsen, R. 1979. The diatom system: ideas on phylogeny. Bacillaria 2: 9–71.
- Smith, J. N. M. & Zach, R. 1979. Heritability of some morphological characters in a song sparrow population. Evolution 33: 460–7.
- Sneath, P. H. & Sokal, R. R. 1973. Numerical Taxonomy. Freeman, San Francisco , 573 pp.
- Soudek, D. & Robinson, G. G. C. 1983. Electrophoretic analysis of the species and population structure of the diatom Asterionella formosa. Can. J. Bot. 61: 418–33.
- Syvertsen, E. E. 1977. Thalassiosira rotula and T. gravida: ecology and morphology. Beih. Nova Hedwigia 54: 99–112.
- Syvertsen, E. E. & Hasle, G. R. 1984. Thalassiosira bulbosa Syvertsen, sp. nov., an arctic marine diatom. Polar Biol. 3: 167–72.
- Tappan, H. 1980. The Paleobiology of Plant Protists. Freeman, San Francisco , 1028 pp.
- Theriot, E. & Stoermer, E. 1984. Principal component analysis of Stephanodiscus: observations on two new species from the Stephanodiscus niagarae complex. Bacillaria 7: 37–58.
- Underhill, P. A. 1977. Nitrate uptake kinetics and clonal variability in the neritic diatom Biddulphia aurtia. J. Phycol. 13: 170–6.
- Von Geissler, U. 1970a. Die Schalenmerkmale der Diatomeen-Ursachen ihrer Variabilität und Bedeutung für die Taxonomie. Beih. Nova Hedwigia 31: 511–35.
- Von Geissler, U. 1970b. Die Variabilität der Schalenmerkmale bei den Diatomeen. Nova Hedwigia 19: 623–73.
- Von Stosch, H. A. & Drebes, G. 1964. Entwicklungsgeschichtliche Untersuchungen an zentrischen Diatomeen. 4. Die Planktondiatomee Stephmopyxis turris, ihre Behandlung und Entwicklungsgeschichte. Helgol. Wiss. Meeresunters. 11: 209–57.
- Walsby, A. E. & Xypolyta, A. 1977. The form resistance of chitan fibres attached to the cells of Thalassiosira fluviatilis Hustedt. Br. Phycol. J. 12: 215–23.
- White, M. J. D. 1978. Modes of Speciation. Freeman, San Francisco , 455 pp.
- Wornardt, Jr. W., 1969. Diatoms, past, present, future. In Brönnimann, P. & Renz, H. H. [Eds.] Proc. 1st Int. Conf. on Planktonic Microfossils, Geneva, 1967. E. J. Brill, Leiden, pp. 690–714.
- Wright, S. 1968. Evolution and the Genetics of Populations. Vol. I Genetics and Biometric Foundations. Univ. Chicago Press, Chicago , 469 pp.
