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Dopamine D₁ Agonist SKF 38393 Increases the State of Phosphorylation of ARPP-21 in Substantia Nigra

Corresponding Author

Kang Tsou

Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, New York, U.S.A.

Address correspondence and reprint requests to Dr. K. Tsou at Box 296, The Rockefeller University, 1230 York Avenue, New York, NY 10021, U.S.A.Search for more papers by this author

Jean-Antoine Girault,

Jean-Antoine Girault

Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, New York, U.S.A.

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Paul Greengard,

Paul Greengard

Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, New York, U.S.A.

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Kang Tsou,

Corresponding Author

Kang Tsou

Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, New York, U.S.A.

Address correspondence and reprint requests to Dr. K. Tsou at Box 296, The Rockefeller University, 1230 York Avenue, New York, NY 10021, U.S.A.Search for more papers by this author

Jean-Antoine Girault,

Jean-Antoine Girault

Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, New York, U.S.A.

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Paul Greengard,

Paul Greengard

Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, New York, U.S.A.

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First published: March 1993

https://doi.org/10.1111/j.1471-4159.1993.tb03252.x

Citations: 14

Dr. J.-A. Girault is INSERM U114, Collège de France, 11 Place Marcelin-Berthelot, 75231 Paris Cedex 05, France.

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Abstract

Abstract: ARPP-21 is a cyclic AMP-regulated phosphoprotein (M_r= 21,000) that has a distribution in brain similar to that of DARPP-32 (dopamine- and cyclic AMP-regulated phosphoprotein, M_r= 32,000). It is enriched in the medium-sized spiny neurons in the striatum and in the striatonigral nerve terminals in the pars reticulata of the substantia nigra. The present study shows that dopamine D₁ agonist SKF 38393 increases the state of phosphorylation of ARPP-21 by 26% in nigral slices and that pretreatment of the slices with D₁ antagonist SCH 23390 blocks this effect. These results demonstrate that ARPP-21 is a dopamine-regulated phosphoprotein. Because D₁ receptors are localized on nerve terminals of striatonigral pathway, the phosphorylation of ARPP-21 is likely to mediate some of the intracellular effects of dopamine on these terminals.

Abbreviation used:

ARPP-21: adenosine 3′,5′-monophosphateregulated phosphoprotein, M_r=21,000 as determined by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (SDS-PAGE)
8-Br-cAMP: 8-bromo-cyclic AMP
cAMP: cyclic AMP
DARPP-32: dopamine- and adenosine 3′,5′-monophosphate-regulated phosphoprotein, M_r=, 32,000 as determined by SDS-PAGE
GABA: γ-aminobutyric acid
IBMX: 3-isobutyl-1-methylxanthine
PMSF: phenylmethylsulfonyl fluoride

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Volume60, Issue3

March 1993

Pages 1043-1046

Dopamine D₁ Agonist SKF 38393 Increases the State of Phosphorylation of ARPP-21 in Substantia Nigra

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Dopamine D1 Agonist SKF 38393 Increases the State of Phosphorylation of ARPP-21 in Substantia Nigra

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Dopamine D₁ Agonist SKF 38393 Increases the State of Phosphorylation of ARPP-21 in Substantia Nigra