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Direct Transfer into Nitrocellulose and Quantitative Radioautographic Anatomical Determination of Brain Tyrosine Hydroxylase Protein Concentration

Corresponding Author

Dinah Weissmann

Laboratoire de Physiologic, Unité de Neuropharmacologie Moléculaire, Faculté Alexis Carrel CNRS URM 12, Lyon, France

Address correspondence and reprint requests to Dr. D. Weissmann at Laboratoire de Physiologie, Unite de Neuropharmacologie Moleculaire, Faculte Alexis Carrel CNRS URM 12, Rue Guillaume Par-adin, 69008. Lyon, France.Search for more papers by this author

René Labatut,

René Labatut

Laboratoire de Physiologic, Unité de Neuropharmacologie Moléculaire, Faculté Alexis Carrel CNRS URM 12, Lyon, France

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Françoise Richard,

Françoise Richard

Laboratoire de Physiologic, Unité de Neuropharmacologie Moléculaire, Faculté Alexis Carrel CNRS URM 12, Lyon, France

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Colette Rousset,

Colette Rousset

Laboratoire de Physiologic, Unité de Neuropharmacologie Moléculaire, Faculté Alexis Carrel CNRS URM 12, Lyon, France

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Jean-François Pujol,

Jean-François Pujol

Laboratoire de Physiologic, Unité de Neuropharmacologie Moléculaire, Faculté Alexis Carrel CNRS URM 12, Lyon, France

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Dinah Weissmann,

Corresponding Author

Dinah Weissmann

Laboratoire de Physiologic, Unité de Neuropharmacologie Moléculaire, Faculté Alexis Carrel CNRS URM 12, Lyon, France

René Labatut,

René Labatut

Laboratoire de Physiologic, Unité de Neuropharmacologie Moléculaire, Faculté Alexis Carrel CNRS URM 12, Lyon, France

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Françoise Richard,

Françoise Richard

Laboratoire de Physiologic, Unité de Neuropharmacologie Moléculaire, Faculté Alexis Carrel CNRS URM 12, Lyon, France

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Colette Rousset,

Colette Rousset

Laboratoire de Physiologic, Unité de Neuropharmacologie Moléculaire, Faculté Alexis Carrel CNRS URM 12, Lyon, France

Search for more papers by this author

Jean-François Pujol,

Jean-François Pujol

Laboratoire de Physiologic, Unité de Neuropharmacologie Moléculaire, Faculté Alexis Carrel CNRS URM 12, Lyon, France

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First published: September 1989

https://doi.org/10.1111/j.1471-4159.1989.tb11775.x

Citations: 30

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Abstract

An improved quantitative immunochemical determination of brain tyrosine hydroxylase (TH) concentra-tions was designed using direct transfer into nitrocellulose from 20-μm thick brain sections, followed by immunodetection and quantitative radioautography in three reference brain structures (locus ceruleus, substantia nigra, and ventral tegmental area). Results obtained by this methodology were similar to those obtained after extraction and Western blotting of the TH protein in control and reserpine-treated animals. Moreover, this methodology allows the combination of high sensitivity and high anatomical resolution in the study of the distribution of pharmacological effects. The locus ceruleus exhibited a significant posteroanterior distribution of TH protein concentration in control and reserpine-treated animals.

Abbreviations used:

BSA: bovine serum albumin
LC: locus ceruleus
OD: optical density
SDS: sodium dodecyl sulfate
SN: substantia nigra
SNC: substantia nigra pars compacta
TBS: Tris-HC1-buffered saline solution
TCA: trichloroacetic acid
TH: tyrosine hydroxylase
U TH/mgt: units of tyrosine hydroxylase per milligram of tissue
VTA: ventral tegmental area

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Volume53, Issue3

September 1989

Pages 793-799

Direct Transfer into Nitrocellulose and Quantitative Radioautographic Anatomical Determination of Brain Tyrosine Hydroxylase Protein Concentration

Abstract

Abbreviations used:

REFERENCES

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Direct Transfer into Nitrocellulose and Quantitative Radioautographic Anatomical Determination of Brain Tyrosine Hydroxylase Protein Concentration

Abstract

Abbreviations used:

REFERENCES

Citing Literature

References

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