Full Access

Phosphatidylinositol Labelling in Response to Activation of Muscarinic Receptors in Bovine Adrenal Medulla

N. A. Mohd. Adnan

Department of Biochemistry, University of Nottinghum Medical School, Queen's Medical Centre, Nottingham NG7 2UH, United Kingdom

Search for more papers by this author

J. N. Hawthorne,

Corresponding Author

J. N. Hawthorne

Department of Biochemistry, University of Nottinghum Medical School, Queen's Medical Centre, Nottingham NG7 2UH, United Kingdom

Address correspondence and reprint requests to Professor J. N. Hawthorne.Search for more papers by this author

N. A. Mohd. Adnan,

N. A. Mohd. Adnan

Department of Biochemistry, University of Nottinghum Medical School, Queen's Medical Centre, Nottingham NG7 2UH, United Kingdom

Search for more papers by this author

J. N. Hawthorne,

Corresponding Author

J. N. Hawthorne

Department of Biochemistry, University of Nottinghum Medical School, Queen's Medical Centre, Nottingham NG7 2UH, United Kingdom

Address correspondence and reprint requests to Professor J. N. Hawthorne.Search for more papers by this author

First published: May 1981

https://doi.org/10.1111/j.1471-4159.1981.tb00441.x

Citations: 37

About

PDF

Tools

Share a link

Email
Wechat
Bluesky

Abstract

Abstract: The secretion of catecholamines and the labelling of phospholipids with ³²P_i has been studied in slices of bovine adrenal medulla. Both nicotinic and muscarinic drugs provoked catecholamine secretion, but only muscarinic activation was accompanied by the increased labelling of phosphatidylinositol and phosphatidic acid.

REFERENCES

Douglas, W. W. (1975) Secretomotor control of adrenal medullary secretion: Synaptic, membrane and ionic events in stimulus-secretion coupling, in Handbook of Physiology, Secfion 7, Vol. 6 ( H. Blaschko, G. Sayers, and A. D. Smith, eds.), pp. 367–380. American Physiological Society, Washington D.C .
Google Scholar
Hokin, M. R., Benfey, B. G., and Hokin, L. E. (1958) Phospholipides and adrenaline secretion in guinea pig adrenal medulla. J. Biol. Chem. 233, 814–817.
CAS PubMed Web of Science® Google Scholar
Jones, L. M., Cockcroft, S., and Michell, R. H. (1979) Stimulation of phosphatidylinositol turnover in various tissues by cholinergic and adrenergic agonists, by histamine and by caerulein. Biochem. J. 182, 669–676.
10.1042/bj1820669
CAS PubMed Web of Science® Google Scholar
Kayaalp, S. O. and Neff, N. H. (1979) Cholinergic muscarinic receptors of bovine adrenal medulla. Neuropharmacology 18, 909–911.
10.1016/0028-3908(79)90090-X
CAS PubMed Web of Science® Google Scholar
Livett, B. G., Kozousek, V., Mizobe, F., and Dean, D. M. (1979) Substance P inhibits nicotinic activation of chromaffin cells. Nature 278, 256–257.
10.1038/278256a0
CAS PubMed Web of Science® Google Scholar
Michell, R. H. (1975) Inositol phospholipids and cell surface receptor function. Biochim. Biophys. Acta 415, 81–147.
10.1016/0304-4157(75)90017-9
CAS PubMed Web of Science® Google Scholar
Pickard, M. R., Hawthorne, J. N., Hayashi, E., and Yamada, S. (1977) Effects of surugatoxin and other nicotinic and muscarinic antagonists on phosphatidylinositol metabolism in active sympathetic ganglia. Biochem. Pharmacol. 26, 448–450.
10.1016/0006-2952(77)90208-8
CAS PubMed Web of Science® Google Scholar
Renzini, V., Brunori, C. A., and Valori, C. (1970) A sensitive and specific fluorimetric method for the determination of noradrenalin and adrenalin in human plasma. Clin. Chim. Acta 30, 587–594.
10.1016/0009-8981(70)90249-4
CAS PubMed Web of Science® Google Scholar
Rouser, G., Fleischer, S., and Yamamoto, A. (1969) Two-dimensional thin-layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots. Lipids 5, 494–496.
10.1007/BF02531316
CAS PubMed Web of Science® Google Scholar
Trifaró, J. M. (1969) The effect of acetylcholine on tissue uptake and incorporation of orthophosphate-³²P into nucleotides and phospholipids of bovine adrenal medulla. Mol. Pharmacol. 5, 382–393.
CAS PubMed Web of Science® Google Scholar
Wilson, S. P. and Kirshner, N. (1977) The acetylcholine receptor of the adrenal medulla. J. Neurochem. 28, 687–695.
10.1111/j.1471-4159.1977.tb10615.x
CAS PubMed Web of Science® Google Scholar
Yagihara, Y., Bleasdale, J. E., and Hawthorne, J. N. (1973) Effects of acetylcholine on the incorporation of [³²P]-orthophosphate in vitro into the phospholipids of subsynaptosomal membranes from guinea-pig brain. J. Neurochem. 21, 173–190.
10.1111/j.1471-4159.1973.tb04237.x
CAS PubMed Web of Science® Google Scholar

Citing Literature

Volume36, Issue5

May 1981

Pages 1858-1860

Phosphatidylinositol Labelling in Response to Activation of Muscarinic Receptors in Bovine Adrenal Medulla

Abstract

REFERENCES

Citing Literature

References

Information

About Wiley Online Library

Help & Support

Opportunities

Connect with Wiley

Phosphatidylinositol Labelling in Response to Activation of Muscarinic Receptors in Bovine Adrenal Medulla

Abstract

REFERENCES

Citing Literature

References

Related

Information