Functional ablation of afferent nerves aggravates dextran sulphate sodium-induced colonic damage in rats
MATTHEW J DOMEK
Research and Medical Services, Sepulveda Veterans Administration Medical Centre, Midway Hospital, UCLA School of Medicine, Los Angeles, CA, USA
Search for more papers by this authorEDWARD I BLACKMAN
Research and Medical Services, Sepulveda Veterans Administration Medical Centre, Midway Hospital, UCLA School of Medicine, Los Angeles, CA, USA
Search for more papers by this authorJOHN KAO
Research and Medical Services, Sepulveda Veterans Administration Medical Centre, Midway Hospital, UCLA School of Medicine, Los Angeles, CA, USA
Search for more papers by this authorXIANG Y ZHANG
Research and Medical Services, Sepulveda Veterans Administration Medical Centre, Midway Hospital, UCLA School of Medicine, Los Angeles, CA, USA
Search for more papers by this authorFUMIHIRO IWATA
Research and Medical Services, Sepulveda Veterans Administration Medical Centre, Midway Hospital, UCLA School of Medicine, Los Angeles, CA, USA
Search for more papers by this authorKYOJI SENO
Research and Medical Services, Sepulveda Veterans Administration Medical Centre, Midway Hospital, UCLA School of Medicine, Los Angeles, CA, USA
Search for more papers by this authorCorresponding Author
FELIX W LEUNG
Research and Medical Services, Sepulveda Veterans Administration Medical Centre, Midway Hospital, UCLA School of Medicine, Los Angeles, CA, USA
Dr Felix W Leung, Division of Gastroenterology (111G) Sepulveda VAMC, 16111 Plummer Street, Sepulveda, CA 91343, USA.Search for more papers by this authorMATTHEW J DOMEK
Research and Medical Services, Sepulveda Veterans Administration Medical Centre, Midway Hospital, UCLA School of Medicine, Los Angeles, CA, USA
Search for more papers by this authorEDWARD I BLACKMAN
Research and Medical Services, Sepulveda Veterans Administration Medical Centre, Midway Hospital, UCLA School of Medicine, Los Angeles, CA, USA
Search for more papers by this authorJOHN KAO
Research and Medical Services, Sepulveda Veterans Administration Medical Centre, Midway Hospital, UCLA School of Medicine, Los Angeles, CA, USA
Search for more papers by this authorXIANG Y ZHANG
Research and Medical Services, Sepulveda Veterans Administration Medical Centre, Midway Hospital, UCLA School of Medicine, Los Angeles, CA, USA
Search for more papers by this authorFUMIHIRO IWATA
Research and Medical Services, Sepulveda Veterans Administration Medical Centre, Midway Hospital, UCLA School of Medicine, Los Angeles, CA, USA
Search for more papers by this authorKYOJI SENO
Research and Medical Services, Sepulveda Veterans Administration Medical Centre, Midway Hospital, UCLA School of Medicine, Los Angeles, CA, USA
Search for more papers by this authorCorresponding Author
FELIX W LEUNG
Research and Medical Services, Sepulveda Veterans Administration Medical Centre, Midway Hospital, UCLA School of Medicine, Los Angeles, CA, USA
Dr Felix W Leung, Division of Gastroenterology (111G) Sepulveda VAMC, 16111 Plummer Street, Sepulveda, CA 91343, USA.Search for more papers by this authorABSTRACT
Dextran sulphate sodium (DSS) is an oral agent capable of inducing chronic diarrhoea and colonic inflammation and necrosis in rats. The role of the afferent nerves in this model of colonic mucosal damage is not known. The hypothesis that functional ablation of the capsaicin-sensitive afferent nerves will aggravate DSS-induced colonic damage in rats was tested. Capsaicin pretreatment was used to ablate afferent nerve function and DSS was administered in the drinking water. Control rats received vehicle pretreatment and water without DSS. There were significant correlations between diarrhoea score, mucosal neutrophil infiltration, mucosal necrosis, and anaemia. Capsaicin pretreatment increased diarrhoea score and colonic mucosal neutrophil infiltration in the rats with colonic damage after 2 or 14 days of DSS. In addition, it induced anaemia and mortality in rats after 14 days of DSS. The data supports the hypothesis that functional ablation of the capsaicin-sensitive afferent nerves aggravates the colonic damage induced by DSS.
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