Prof. Sun Chul Kang, Department of Biotechnology, College of Engineering, Daegu University, Kyoungsan, Kyoungbook 712 714, Korea.
Tel.: +82 53 850 6553;
Fax: +82 53 850 6559;
E-mail: [email protected]

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Summary

The present study aimed at investigating the effects of essential oil of Trachyspermum ammi fruits, an oil-bearing plant of Apiaceae family, on human sperm viability and membrane integrity. Chemical compositions of the oil were analysed by GC-MS. Thirty compounds representing 91.39% of the total oil were identified. The viability and membrane integrity of human spermatozoa were assessed using minimum effective dose (MED) concentration (125 μg ml⁻¹) of the oil. Sperm treated with essential oil showed a significant decrease (P < 0.05) in viability assessed by eosin–nigrosin and fluorescence dual staining. Moreover, the treated sperm also showed a significant loss (P < 0.05) of functional mitochondria and antioxidant enzyme, catalase (EC 1.11.1.6, CAT), when compared to control. The cholesterol:phospholipid ratio was also increased (P < 0.05) in treated sperm when compared to control, which is an indicator of loss of binding ability of human spermatozoa to the zona pellucida. The scanning electron microscopic studies demonstrated the loss of membrane integrity in essential oil–treated human spermatozoa, which showed vacuolation, swelling of acrosomal cap, detachment of head portion and tail coiling. Present observations indicate the spermicidal property of essential oil of T. ammi fruits, which could be helpful to develop medicinal preparations as a male contraceptive.

References

Adams RP (2001) Identification of Essential Oil Components by Gas Chromatography/Quadrupole Mass Spectroscopy. Allured Publishing Corporation, Carol stream, IL, USA.
Google Scholar
Arora DS, Kaur GJ (2007) Antibacterial activity of some Indian medicinal plants. J Nat Med 61: 313–317.
10.1007/s11418-007-0137-8
Web of Science® Google Scholar
Bartlett GR (1959) Phosphorus assay in column chromatography. J Biol Chem 243: 466–468.
Google Scholar
Bhargava PP, Chowdary BL, Haskar CM (1963) Essential oil from Ajwin. Perfume Essent Oil Res 54: 740.
Google Scholar
Brinsko SP, Love CC, Bauer JE, Macpherson ML, Varner DD (2007) Cholesterol-to-phospholipid ratio in whole sperm and seminal plasma from fertile stallions and stallions with unexplained subfertility. Ani Reprod Sci 99: 65–71.
10.1016/j.anireprosci.2006.03.018
CAS PubMed Web of Science® Google Scholar
Chaki SP, Misro MM (2002) Assessment of human sperm function after hydrogen peroxide exposure: development of a vaginal contraceptive. Contraception 66: 187–192.
10.1016/S0010-7824(02)00349-9
CAS PubMed Web of Science® Google Scholar
Chakraborti K, Pal S, Bhattacharya AK (2003) Sperm immobilization activity of Allium sativum L and other plant extracts. Asian J Androl 5: 131–135.
PubMed Web of Science® Google Scholar
Chang MC (1957) A detrimental effect of seminal plasma on the fertilizing capacity of sperm. Nature 179: 258–259.
10.1038/179258a0
CAS PubMed Web of Science® Google Scholar
Chaudhury K, Bhattacharyya AK, Guha SK (2004) Studies on the membrane integrity of human sperm treated with a new injectable male contraceptive. Hum Reprod 19: 1826–1830.
10.1093/humrep/deh332
CAS PubMed Web of Science® Google Scholar
Choudhury S, Ahmed R, Kanjilal PB, Leclercq PA (1998) Composition of the seed oil of Trachyspermum ammi (L.) Sprague from Northeast India. J Essent Oil Res 10: 588–590.
10.1080/10412905.1998.9700979
CAS Google Scholar
Cross NL (1996) Human seminal plasma prevents sperm from becoming acrosomally responsive to the agonist, progesterone: cholesterol is the major inhibitor. Biol Reprod 54: 138–145.
10.1095/biolreprod54.1.138
CAS PubMed Web of Science® Google Scholar
Evenson DP, Darzynkiewicz Z, Melamed MR (1982) Simultaneous measurement by flow cytometry of sperm cell viability and mitochondrial membrane potential related to cell motility. J Histochem Cytochem 30: 279–280.
10.1177/30.3.6174566
CAS PubMed Web of Science® Google Scholar
Evenson DP, Larson KL, Jost LK (2002) Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques. J Androl 23: 25–43.
10.1002/j.1939-4640.2002.tb02599.x
PubMed Web of Science® Google Scholar
Farnsworth NR, Waller DP (1982) Current status of plant products reported to inhibit sperm. Res Front Fertil Regul 2: 1–16.
CAS PubMed Google Scholar
Fichorova RN, Tucker LD, Anderson DJ (2001) The molecular basis of nonoxynol-9 induced vaginal inflammation and its possible relevance to human immunodeficiency virus type 1 transmission. J Infect Dis 184: 418–428.
10.1086/322047
CAS PubMed Web of Science® Google Scholar
Flaherty CO, De Lamirande E, Gagnon C (2006) Positive role of reactive oxygen species in mammalian sperm capacitation: triggering and modulation of phosphorylation events. Free Radic Biol Med 41: 528–540.
10.1016/j.freeradbiomed.2006.04.027
CAS PubMed Web of Science® Google Scholar
Folch J, Lees M, Sloane-Stanely GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226: 497–509.
10.1016/S0021-9258(18)64849-5
CAS PubMed Web of Science® Google Scholar
Freeman A, Crapo D (1982) Biology of disease-free radicals and tissue injury. Lab Invest 47: 412–426.
CAS PubMed Web of Science® Google Scholar
Fujita T, Miyoshi M, Tokunaga J (1970) Scanning and transmission electron microscopy of human ejaculate spermatozoa with special reference to their abnormal forms. Z Zellforsch 105: 483–497.
10.1007/BF00335423
CAS PubMed Web of Science® Google Scholar
Gamzu R, Yogev L, Paz G, Yavetz H, Lichtenberg D (1997) Reduction of sperm cholesterol:phospholipid ratio is a possible mechanism for enhancement of human sperm binding to the zona pellucida following incubation with phosphatidylcholine liposomes. Biol Reprod 57: 539–546.
10.1095/biolreprod57.3.539
CAS PubMed Web of Science® Google Scholar
Garg S, Taluja V, Upadhyay SN, Talwar GP (1993) Studies on the contraceptive efficacy of Praneem polyherbal cream. Contraception 48: 591–596.
10.1016/0010-7824(93)90120-V
CAS PubMed Web of Science® Google Scholar
Go′th L (1991) A simple method for determination of serum catalase activity and revision of reference range. Clin Chim Acta 196: 143–151.
10.1016/0009-8981(91)90067-M
CAS PubMed Web of Science® Google Scholar
Gopalkrishnan K (1995) Standardized procedures in human semen analysis. Curr Sci 68: 353–362.
Web of Science® Google Scholar
Haeri S, Minaie B, Amin G, Nikfar S, Khorasani R, Esmaily H, Salehnia A, Abdollahi M (2006) Effect of Satureja khuzestanica essential oil on male rat fertility. Contraception 77: 495–499.
CAS PubMed Web of Science® Google Scholar
Hoshi K, Aita T, Yanagida K, Yoshimatsu N, Sato A (1990) Variation in the cholesterol/phospholipids ratio in human spermatozoa and its relationship with capacitation. Hum Reprod 5: 71–74.
10.1093/oxfordjournals.humrep.a137045
CAS PubMed Web of Science® Google Scholar
Khillare B, Shrivastav TG (2003) Spermicidal activity of Azadirachta indica (neem) leaf extract. Contraception 68: 225–229.
10.1016/S0010-7824(03)00165-3
CAS PubMed Web of Science® Google Scholar
Kono Y, Fridovich I (1982) Superoxide radical inhibits catalase. J Biol Chem 257: 5751–5754.
10.1016/S0021-9258(19)83842-5
CAS PubMed Web of Science® Google Scholar
Lange Y, Ye J, Stech TL (2005) Activation of membrane cholesterol by displacement from phospholipids. J Biol Chem 280: 126–131.
10.1074/jbc.M507149200
CAS Web of Science® Google Scholar
Lohiya NK, Ansari AS (1999) Male contraceptive agents. In: Comparative Endocrinology and Reproduction. KP Joy, A Krishna, C Haldar (eds). Narosa Publishing House, New Delhi, pp. 260–277.
Google Scholar
Lohiya NK, Kothari LK, Manivannan B, Mishra PK, Pathak N (2000) Human sperm immobilization effect of Carica papaya seed extracts: an in vitro study. Asian J Androl 2: 103–109.
CAS PubMed Web of Science® Google Scholar
Mahadevan MM, Miller MM, Moutos DM (1997) Absence of glucose decreases human fertilization and sperm movement characteristic in vitro. Hum Reprod 12: 119–123.
10.1093/humrep/12.1.119
CAS PubMed Web of Science® Google Scholar
Manicardi G, Bianch B, Pantono S (1995) Underprotamination and nicking of DNA in ejaculated human spermatozoa are highly related phenomena. Biol Reprod 55: 864–867.
10.1095/biolreprod52.4.864
Web of Science® Google Scholar
McGee RS, Herr JC (1987) Human seminal vesicle-specific antigen during semen liquefaction. Biol Reprod 37: 431–439.
10.1095/biolreprod37.2.431
CAS PubMed Web of Science® Google Scholar
Pandey SK, Upadhyay S, Tripathi AK (2003) Insecticidal and repellent activities of thymol from the essential oil of Trachyspermum ammi (Linn) Sprague seeds against Anopheles stephensi. Parasitol Res 105: 507–512.
10.1007/s00436-009-1429-6
Web of Science® Google Scholar
Parks JE, Lynch DV (1992) Lipid composition and thermotrophic phase behavior of boar, bull, stallion, and rooster sperm membranes. Cryobiology 29: 255–266.
10.1016/0011-2240(92)90024-V
CAS PubMed Web of Science® Google Scholar
Paul D, Bera S, Jana D, Maiti R, Ghosh D (2006) In vitro determination of the contraceptive spermicidal activity of a composite extract of Achyranthes aspera and Stephania hernandifolia on human semen. Contraception 73: 284–288.
10.1016/j.contraception.2005.07.014
PubMed Web of Science® Google Scholar
Primorac M, Sekulovic D, Antonic S (1985) In vitro determination of the spermicidal activity of plant saponins. Pharmazie 40: 585.
CAS PubMed Web of Science® Google Scholar
Shariff ZU (2001) Modern herbal therapy for common aliments. Nature Pharmacy Series, Volume 1. Spectrum Books Limited, in association with United Kingdom: Safari Books (Export) Limited, Ibadan, Nigeria. pp 9–84.
Google Scholar
Sharma SK, SaiRam M, Ilvazhagan G, Devendra K, Shivaji SS, Selvamurthy W (1996) Mechanism of action of NIM-76: a novel vaginal contraceptive from neem oil. Contraception 54: 373–378.
10.1016/S0010-7824(96)00204-1
CAS PubMed Web of Science® Google Scholar
Sun XM, MacFarlane M, Zhuang J, Wolf BB, Green DR, Cohe GM (1999) Distinct caspase cascades are initiated in receptor-mediated and chemical-induced apoptosis. J Biol Chem 274: 5053–5060.
10.1074/jbc.274.8.5053
CAS PubMed Web of Science® Google Scholar
Tejada RI, Mitchell JC, Norman A, Marik JJ, Friedman S (1984) A test for the practical evaluation of male fertility by acridine orange (AO) fluorescence. Fertil Steril 42: 87–91.
10.1016/S0015-0282(16)47963-X
CAS PubMed Web of Science® Google Scholar
Twigg JP, Irvine DS, Aitken RJ (1998) Oxidative damage to DNA in human spermatozoa does not preclude pronucleus formation at intracytoplasmic sperm injection. Hum Reprod 13: 1864–1871.
10.1093/humrep/13.7.1864
CAS PubMed Web of Science® Google Scholar
Vis EH, Geerse GJ, Klaassens ES, van Boekel MA, Alink GM (2005) Possible mechanisms behind the differential effects of soy protein and casein feedings on colon cancer biomarkers in the rat. Nutr Cancer 51: 37–44.
10.1207/s15327914nc5101_6
CAS PubMed Web of Science® Google Scholar
Weir SS, Roddy RE, Zekeng L, Feldblum PJ (1995) Nonoxynol-9 use, genital ulcers, and HIV infection in a cohort of sex workers. Genet Med 71: 78–81.
CAS PubMed Web of Science® Google Scholar
WHO (1999) Laboratory Manual for the Examination of Human Semen and Sperm–Cervical Mucus Interaction, 4th edn. Cambridge University Press, New York.
Google Scholar
WHO (2000) Reproductive health research at WHO: a new beginning, Biennial Report 1998–99. Special Programme of Research, Development and Research Training in Human Reproduction, World Health Organization, Geneva.
Google Scholar
Wilborn WH, Mahn DW, Mcguire JJ (1983) Scanning electron microscopy of human spermatozoa after incubation with the spermicidal nonoxynol-9. Fertil Steril 39: 717–719.
10.1016/S0015-0282(16)47074-3
CAS PubMed Web of Science® Google Scholar

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Studies on the viability and membrane integrity of human spermatozoa treated with essential oil of Trachyspermum ammi (L.) Sprague ex Turrill fruit

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Studies on the viability and membrane integrity of human spermatozoa treated with essential oil of Trachyspermum ammi (L.) Sprague ex Turrill fruit

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