Volume 104, Issue 4 pp. 265-271

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Fusicoccin Binding to its Plasma Membrane Receptor and the Activation of the Plasma Membrane H⁺-ATPase. II. Stimulation of the H⁺-ATPase in a Plasma Membrane Fraction Purified by Phase-partitioning

Maria Ida De Michelis,

Maria Ida De Michelis

Istituto di Botanica, Università di Messina, Messina-S. Agata, Italy.

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Franca Rasi-Caldogno,

Corresponding Author

Franca Rasi-Caldogno

Centro di Studio del C.N.R. sulla Biologia Cellulare e Molecolare delle Piante, Dipartimento di Biologia, Università degli Studi di Milano, Italy.

Dipartimento di Biologia Università di Milano Via G. Celoria, 26 I-20133 Milano ItalySearch for more papers by this author

Maria Chiara Pugliarello,

Maria Chiara Pugliarello

Centro di Studio del C.N.R. sulla Biologia Cellulare e Molecolare delle Piante, Dipartimento di Biologia, Università degli Studi di Milano, Italy.

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C. Olivari,

C. Olivari

Centro di Studio del C.N.R. sulla Biologia Cellulare e Molecolare delle Piante, Dipartimento di Biologia, Università degli Studi di Milano, Italy.

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Maria Ida De Michelis,

Maria Ida De Michelis

Istituto di Botanica, Università di Messina, Messina-S. Agata, Italy.

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Franca Rasi-Caldogno,

Corresponding Author

Franca Rasi-Caldogno

Centro di Studio del C.N.R. sulla Biologia Cellulare e Molecolare delle Piante, Dipartimento di Biologia, Università degli Studi di Milano, Italy.

Dipartimento di Biologia Università di Milano Via G. Celoria, 26 I-20133 Milano ItalySearch for more papers by this author

Maria Chiara Pugliarello,

Maria Chiara Pugliarello

Centro di Studio del C.N.R. sulla Biologia Cellulare e Molecolare delle Piante, Dipartimento di Biologia, Università degli Studi di Milano, Italy.

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C. Olivari,

C. Olivari

Centro di Studio del C.N.R. sulla Biologia Cellulare e Molecolare delle Piante, Dipartimento di Biologia, Università degli Studi di Milano, Italy.

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First published: August 1991

https://doi.org/10.1111/j.1438-8677.1991.tb00228.x

Citations: 22

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Abstract

The effect of fusicoccin (FC) on the activity of the PM H⁺-ATPase was investigated in a plasma membrane (PM) fraction from radish seedlings purified by the phase-partitioning procedure.

FC stimulated the PM H⁺-ATPase activity by up to 100 %; the effect was essentially on V_max with only a slight decrease of the apparent K_M of the enzyme for ATP.

FC-induced stimulation of the PM H⁺-ATPase was evident within the first minute and maximal within five minutes of membrane treatment with the toxin indicating that transmission of the signal from the activated receptor to the PM H⁺-ATPase is very rapid.

Both FC-induced stimulation of the PM H⁺-ATPase and FC binding to its receptor decreased dramatically upon incubation of the membranes in ATPase assay medium at 33 °C in the absence of FC, due to the lability of the free FC receptor.

FC-induced stimulation of the PM H⁺-ATPase was strongly pH dependent: absolute increase of activity was maximal at pH 7, while percent stimulation increased with the increase of pH up to pH 7.5; FC binding was scarcely influenced by pH in the pH range investigated. Taken as a whole, these results indicate that FC binding is a condition necessary, but not sufficient, for FC-induced stimulation of the PM H⁺-ATPase.

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Volume104, Issue4

August 1991

Pages 265-271

Fusicoccin Binding to its Plasma Membrane Receptor and the Activation of the Plasma Membrane H⁺-ATPase. II. Stimulation of the H⁺-ATPase in a Plasma Membrane Fraction Purified by Phase-partitioning

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Fusicoccin Binding to its Plasma Membrane Receptor and the Activation of the Plasma Membrane H+-ATPase. II. Stimulation of the H+-ATPase in a Plasma Membrane Fraction Purified by Phase-partitioning

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Fusicoccin Binding to its Plasma Membrane Receptor and the Activation of the Plasma Membrane H⁺-ATPase. II. Stimulation of the H⁺-ATPase in a Plasma Membrane Fraction Purified by Phase-partitioning