Full Access
THE «ANOMALOUS» 304nm EXTREMUM IN THE THERMAL PERTURBATION OF TRYPTOPHAN
Absence of a Fluorescence Artifact
First published: January 1978
Abstract
Thermal perturbation spectra of α-chymotrypsinogen with and without the fluorescence quencher Eu(NO3)3 show that the «anomalous» 305 nm extremum (presumed to arise from tryptophan residues) is not a fluorescence artifact. A similar result was obtained for Nα-acetyltryptophan amide in 13.6 M potassium formate.
