STUDIES ON POLYPEPTIDES:
XXIV. Synthesis of the 5–17 and 1–17 Peptide Sequences of Rat Pancreatic Ribonuclease.*
All amino acids, except glycine, were of the L-conifiguration. The following abbreviations are used: RNase S, the subtilisin modified bovine pancreatic ribonuclease; S-protein, the protein component obtained from RNase S; S-peptide, the polypeptide obtained from RNase S; RNase S‘, equimolar combination of S-peptide and S-protein; RNA, yeast ribonucleic acid. The abbreviations for the amino acids and protecting groups are those recommended by the IUPAC-IUB Commission on Biochemical Nomenclature, Biochemistry 11, (1972) 1726–1732.
Abstract
A description is given of the synthesis by fragment condensation of the peptides Glu-Ser-Ser-Ala-Asp-Lys-Phe-Lys-Arg-Gln-His-Met-Asp and Gly-Glu-Ser-Arg-Glu-Ser-Ser-Ala-Asp-Lys-Phe-Lys-Arg-Gln-His-Met-Asp respectively corresponding to the 5–17 and 1–17 amino acid sequences of rat pancreatic ribonuclease.