STUDIES ON CYTOCHROME C:
XII. Synthesis of the Protected Undecapeptide (Sequence 66–76) and Pentadecapeptide (Sequence 66–80) of Horse Heart Cytochrome c*
The amino acid residues, except glycine, are of the L-configuration. For a simpler description the customary L-designation for individual amino acid residues is omitted. Standard abbreviations for amino acid derivatives and peptides are used according to the suggestion of the IUPAC-IUB Commission on Biochemical Nomenclature (1972) Biochemistry 11, 1726–1732.
Abstract
This paper is part of a series on synthesis of suitably protected peptides covering the 66–104 sequence of horse heart cytochrome c. It describes the preparation, by conventional procedures, of a partially protected Nα-benzyloxycarbonyl-undecapeptide hydrazide corresponding to the sequence from 66 to 76 (Fragment F), which represents a building block for the synthesis of the entire 66 – 104 sequence.
Moreover, the preparation is described of a partially protected pentadecapeptide corresponding to the sequence region 66 to 80, which represents the key peptide for the semisynthesis of the same COOH-terminal sequence utilizing the natural 81 – 104 N-trifluoroacetylated CNBr fragment.
