Frequency of the phenylalanine deletion (ΔF508) in the CF gene of Belgian cystic fibrosis patients
Jan G. Wauters
Department of Medical Genetics, University of Antwerp-U.I.A.
Search for more papers by this authorJan Hendrickx
Department of Medical Genetics, University of Antwerp-U.I.A.
Search for more papers by this authorPaul Coucke
Department of Medical Genetics, University of Antwerp-U.I.A.
Search for more papers by this authorLieve Vits
Department of Medical Genetics, University of Antwerp-U.I.A.
Search for more papers by this authorKarel Stuer
Department of Medical Genetics, University of Antwerp-U.I.A.
Search for more papers by this authorBart J. van der Auwera
Department of Medical Genetics, University of Antwerp-U.I.A.
Search for more papers by this authorAugust Van Elsen
Department of Medical Genetics, University of Antwerp-U.I.A.
Search for more papers by this authorJan Dumon
Department of Medical Genetics, University of Antwerp-U.I.A.
Search for more papers by this authorCorresponding Author
Patrick J. Willems
Department of Medical Genetics, University of Antwerp-U.I.A.
Universitaire Instelling Antwerpen Universiteitsplein 1 2610 Wilrijk BelgiumSearch for more papers by this authorJan G. Wauters
Department of Medical Genetics, University of Antwerp-U.I.A.
Search for more papers by this authorJan Hendrickx
Department of Medical Genetics, University of Antwerp-U.I.A.
Search for more papers by this authorPaul Coucke
Department of Medical Genetics, University of Antwerp-U.I.A.
Search for more papers by this authorLieve Vits
Department of Medical Genetics, University of Antwerp-U.I.A.
Search for more papers by this authorKarel Stuer
Department of Medical Genetics, University of Antwerp-U.I.A.
Search for more papers by this authorBart J. van der Auwera
Department of Medical Genetics, University of Antwerp-U.I.A.
Search for more papers by this authorAugust Van Elsen
Department of Medical Genetics, University of Antwerp-U.I.A.
Search for more papers by this authorJan Dumon
Department of Medical Genetics, University of Antwerp-U.I.A.
Search for more papers by this authorCorresponding Author
Patrick J. Willems
Department of Medical Genetics, University of Antwerp-U.I.A.
Universitaire Instelling Antwerpen Universiteitsplein 1 2610 Wilrijk BelgiumSearch for more papers by this authorAbstract
Cloning and sequencing of the CF gene has identified a three-base-pair deletion (ΔF508) responsible for CF in the majority of CF patients (Kerem et al. 1989). We have used the polymerase chain reaction with oligonucleotide primers bridging the ΔF308 deletion to analyze the presence or absence of this mutation in the Belgian CF population. The ΔF306 mutation was present in 80% (57 on 71) of CF chromosomes from 36 unrelated Belgian CF families from the region of Antwerp. This mutation was associated with haplotype B for the KM.19–XV-2c RFLPs as 93% (53 on 57) of the CF chromosomes with the Δ306 mutation carried haplotype B.
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