Inhibition of equine neutrophil chemotaxis and chemokinesis by a Taenia taeniaeformis proteinase inhibitor, taeniaestatin
Corresponding Author
R. W. LEID
Laboratory of Molecular and Biochemical Parasitology, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
Dr R. Wes Leid, Laboratory of Molecular and Biochemical Parasitology, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164–7040, USASearch for more papers by this authorR. F. GRANT
Laboratory of Molecular and Biochemical Parasitology, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
Search for more papers by this authorCHRISTINE M. SUQUET
Laboratory of Molecular and Biochemical Parasitology, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
Search for more papers by this authorCorresponding Author
R. W. LEID
Laboratory of Molecular and Biochemical Parasitology, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
Dr R. Wes Leid, Laboratory of Molecular and Biochemical Parasitology, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164–7040, USASearch for more papers by this authorR. F. GRANT
Laboratory of Molecular and Biochemical Parasitology, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
Search for more papers by this authorCHRISTINE M. SUQUET
Laboratory of Molecular and Biochemical Parasitology, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
Search for more papers by this authorAbstract
Summary Taeniaestatin, a recently isolated Taenia taeniaeformis proteinase inhibitor, was used to inhibit equine neutrophil migration. Taeniaestatin itself was not chemotactic when used as a chemotactic factor but taeniaestatin did inhibit neutrophil chemokinesis when tested in a Zigmond-Hirsch checkerboard assay. A dose-dependent inhibition of both chemokinesis and chemotaxis was observed when zymosan activated bovine sera (ZABS) was used as the chemotactic factor. This inhibition was > 95% when 5 u of taeniaestatin was present on both the cell and chemotactic factor side of the chambers. Equine neutrophils gave dose- and time-dependent migration responses to purified bovine C5a with an ED50 of 1±04 ± 10–7 M. Taeniaestatin inhibited the C5a-mediated chemotactic and chemokinetic neutrophil responses (51% using 1 u and > 95% with 5 u of taeniaestatin). The inhibition of leucocyte motility by taeniaestatin was reversible and without cytotoxicity at the highest doses of taeniaestatin tested.
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