Effects of octreotide on jejunal hypersensitivity triggered by Cryptosporidium parvum intestinal infection in an immunocompetent suckling rat model
J. Bai
Parasitology Department, Rouen University Hospital and Institute for Biomedical Research, University of Rouen, Rouen, France
Department of Pharmacology, Xinjiang Medical University, Urumqi, Xinjiang, China
Search for more papers by this authorS. Khaldi
Parasitology Department, Rouen University Hospital and Institute for Biomedical Research, University of Rouen, Rouen, France
Search for more papers by this authorG. Gargala
Parasitology Department, Rouen University Hospital and Institute for Biomedical Research, University of Rouen, Rouen, France
Search for more papers by this authorA. Baishanbo
Department of Pharmacology, Xinjiang Medical University, Urumqi, Xinjiang, China
Search for more papers by this authorA. François
Department of Histopathology, Rouen University Hospital and University of Rouen, Rouen, France
Search for more papers by this authorJ. J. Ballet
Department of Immunology, Caen University Hospital and University of Caen, Caen, France
Search for more papers by this authorP. Ducrotté
Gastroenterology Unit, Rouen University Hospital and Institute for Biomedical Research, University of Rouen, Rouen, France
Search for more papers by this authorJ. Fioramonti
Neurogastroenterology and Nutrition Unit, INRA, Toulouse, France
Search for more papers by this authorL. Favennec
Parasitology Department, Rouen University Hospital and Institute for Biomedical Research, University of Rouen, Rouen, France
Search for more papers by this authorL. Le Goff
Parasitology Department, Rouen University Hospital and Institute for Biomedical Research, University of Rouen, Rouen, France
Search for more papers by this authorJ. Bai
Parasitology Department, Rouen University Hospital and Institute for Biomedical Research, University of Rouen, Rouen, France
Department of Pharmacology, Xinjiang Medical University, Urumqi, Xinjiang, China
Search for more papers by this authorS. Khaldi
Parasitology Department, Rouen University Hospital and Institute for Biomedical Research, University of Rouen, Rouen, France
Search for more papers by this authorG. Gargala
Parasitology Department, Rouen University Hospital and Institute for Biomedical Research, University of Rouen, Rouen, France
Search for more papers by this authorA. Baishanbo
Department of Pharmacology, Xinjiang Medical University, Urumqi, Xinjiang, China
Search for more papers by this authorA. François
Department of Histopathology, Rouen University Hospital and University of Rouen, Rouen, France
Search for more papers by this authorJ. J. Ballet
Department of Immunology, Caen University Hospital and University of Caen, Caen, France
Search for more papers by this authorP. Ducrotté
Gastroenterology Unit, Rouen University Hospital and Institute for Biomedical Research, University of Rouen, Rouen, France
Search for more papers by this authorJ. Fioramonti
Neurogastroenterology and Nutrition Unit, INRA, Toulouse, France
Search for more papers by this authorL. Favennec
Parasitology Department, Rouen University Hospital and Institute for Biomedical Research, University of Rouen, Rouen, France
Search for more papers by this authorL. Le Goff
Parasitology Department, Rouen University Hospital and Institute for Biomedical Research, University of Rouen, Rouen, France
Search for more papers by this authorAbstract
Background Similar to other bacterial or protozoan infections, human cryptosporidiosis may trigger postinfectious irritable bowel syndrome (IBS)-like symptoms, a condition in which enhanced visceral perception of pain during intestinal distension plays a pivotal role. In an immunocompetent suckling rat model which mimicks features of postinfectious IBS, Cryptosporidium parvum infection induces long-lasting jejunal hypersensitivity to distension in association with intestinal activated mast cell accumulation. The aim of the present study was to explore in this model whether octreotide, a somatostatin agonist analog, could prevent the development of jejunal hypersensitivity and intestinal mast cell/nerve fiber accumulation.
Methods Five-day-old Sprague–Dawley rats were infected with C. parvum and treated 10 days later with octreotide (50 g kg−1day−1, i.p.) for 7 days.
Key Results Compared with untreated infected rats, octreotide treatment of infected rats resulted in increased weight gain [day 23 postinfection (PI)], decreased food intake (day 16 PI), and a reduction in jejunal villus alterations (day 14 PI), CD3+IEL (day 37 PI) and mast cell (days 37 and 50 PI) accumulations, nerve fiber densities (day 50 PI), and hypersensitivity to distension (day 120 PI). In uninfected rats, the effects of octreotide treatment were limited to higher weight gain (days 16 and 23 PI) and decreased food intake (day 23 PI) compared with uninfected-untreated rats.
Conclusions & Inferences Data confirms the relevance of the present rat model to postinfectious IBS studies and prompt further investigation of somatostatin-dependent regulatory interactions in cryptosporidiosis.
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